Gıda Flavonoidlerinin Yüksek Basınç Sıvı Kromatografisi İle Analizi

Yıl 2004, Cilt: 2 Sayı: 2, 24 - 27, 01.04.2004

Mustafa Çam Yaşar Hışıl

Öz

Flavonoids are the most common and widely distributed group of polyphenolic compounds. Over 5000 different flavonoids have been described in literature and they are generally classified into 6 chemical groups anthocyanidins, flavanones, flavones, flavonols, flavanols isoflavones . Flavonoids are found in seeds, fruits, leaves and flowers of plants, especially in the form of glycosides. Although flavonoids are non-nutritive agents, the health effects of flavonoids in human diet have attracted much attention. They show powerful antioxidant activity which can protect the human body from free radicals. Among the chromatographic methods, high pressure liquid chromatography is the most widely used method for the analysis of flavonoids. Many systems have been developed for the analysis of flavonoids together one, two, or three subclasses. The methods that have been developed to analysis of flavonoids by high pressure liquid chromatography and sample preperation procedures are summarized in this review

Anahtar Kelimeler

Flavonoids, HPLC, phenolic compounds

Gıda Flavonoidlerinin Yüksek Basınç Sıvı Kromatografisi İle Analizi

Öz

Polifenolik bileşiklerden flavonoidler bitkilerde en çok ve en yaygın şekilde bulunan bileşiklerdir. Şu ana kadar 5000 den fazla flavonoid bileşiği tanımlanmıştır ve bunlar genelde 6 kimyasal grup olarak sınıflanmıştır antosiyanidinler, flavanonlar, flavonlar, flavonoller, flavanoller, izoflavonlar . Bitkilerin yaprak, tohum, kabuk ve çiçeklerinde ve genellikle glikozitleri şeklinde bulunurlar. Tıbbi açıdan pek çok öneme sahip bitkide flavonoidlerin aktif bileşenler olduğu düşünülmektedir. Besinsel fonksiyonları olmamasına rağmen diyetteki flavonoidlerin sağlık üzerine olumlu etkileri sebebiyle dikkat çekmektedirler.İnsan vücudunu serbest radikallere karşı koruyarak antioksidan aktivite gösterirler. Kromatografik yöntemler arasında yüksek basınç sıvı kromatografisi flavonoidlerin analizinde en sık kullanılan yöntemdir. Flavonoid analizlerinde bir, iki ya da üç alt sınıfın bir arada analizinin yapıldığı pek çok yöntem geliştirilmiştir. Bu çalışmada flavonoidlerin yüksek basınç sıvı kromatografisi ile analizinde kullanılan yöntemler ve örnek hazırlama işlemleri özetlenmiştir

Anahtar Kelimeler

Flavonoidler, HPLC, fenolik bileşikler

Kaynakça

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