Hesperidin Alleviates Cecal Ligation and Puncture-Induced Lung and Kidney Injuries

Abstract

ABSTRACT Objective: The potential useful features of hesperidin (Hes) was examined against lung and kidney injuries triggered by cecal ligation and puncture (CLP) in rats in current study. Materials and Methods: 32 Wistar Albino male rats were randomly allocated as sham, CLP, Hes 100 mg/kg and Hes 200 mg/kg groups. For the CLP process under anesthesia, the abdominal area was shaved and cleaned. The cecum was tied with a 4.0 suture and pierced by 18-gauge needle. Using this method, the experimental sepsis model was created. The lung and kidney tissues were removed at the end of the experiment. Biochemical and immunohistochemical analyzes were performed. Hes was administered by oral gavage at the doses of 100 and 200 mg/kg for 15 days. Results: Total oxidant status (TOS), malondialdehyde (MDA) and oxidative stress index (OSI) levels, myeloperoxidase (MPO) activity raised significantly while superoxide dismutase (SOD) and total antioxidant status (TAS) values declined in CLP group compared to sham group in lung and kidney tissues. On the contrary, SOD and TAS levels increased while MPO activity, TOS, OSI and MDA levels decreased due to Hes treatments. Caspase-3 and LC3B immunopositivity raised significantly in CLP group compared to sham group in lung and kidney tissues while decreased was observed in Hes treatment groups. Conclusion: In this study, in the light of our biochemical and immunohistochemical results that it has been detected that, Hes demonstrated an effective protection against CLP-induced lung and kidney tissue injuries in rats. Key Words: Sepsis, Hesperidin, Lung, Kidney, Oxidative stress, Autophagy

Keywords

• Sepsis
• Hesperidin
• Lung
• Kidney
• Oxidative stress
• Autophagy

Hesperidin, Çekal Ligasyonu ve Delinmeye Bağlı Akciğer ve Böbrek Yaralanmalarını Hafifletir

Öz

ÖZ Amaç: Bu çalışmada, sıçanlarda çekal ligasyon ve delme (CLP) ile tetiklenen akciğer ve böbrek hasarlarına karşı hesperidinin (Hes) potansiyel yararlı özellikleri incelenmiştir. Gereç ve Yöntemler: 32 adet Wistar Albino erkek rat rastgele olarak sham, CLP, Hes 100 mg/kg ve Hes 200 mg/kg gruplarına ayrıldı. Anestezi altında CLP işlemi için karın bölgesi tıraş edilerek temizlendi. Çekum 4,0 sütür ile bağlandı ve 18' lik iğne ile delindi. Bu metod kullanılarak deneysel sepsis modeli oluşturuldu. Deneyin sonunda akciğer ve böbrek dokuları çıkarıldı. Biyokimyasal ve immünohistokimyasal analizler yapıldı. Hes, 15 gün süreyle 100 ve 200 mg/kg dozlarında oral gavaj ile uygulandı. Bulgular: Sham grubu CLP grubu ile kıyaslandığında akciğer ve böbrek dokularında Total oksidan durum (TOS), malondialdehit (MDA) ve oksidatif stres indeksi (OSI) düzeyleri, miyeloperoksidaz (MPO) aktivitesi anlamlı olarak artarken süperoksit dismutaz (SOD) ve total antioksidan durum (TAS) değerleri azaldı. Bunun aksine, Hes tedavilerine bağlı olarak SOD ve TAS seviyeleri artarken MPO aktivitesi, TOS, OSI ve MDA seviyeleri azalmıştır. Sham grubu CLP grubu ile kıyaslandığında akciğer ve böbrek dokularında Kaspaz-3 ve LC3B immünopozitifliği anlamlı olarak artarken, Hes tedavi gruplarında azalma gözlendi. Sonuç: Bu çalışmada, sunduğumuz biyokimyasal ve immünohistokimyasal bulgularımız ışığında, Hes' in sıçanlarda CLP' ye bağlı akciğer ve böbrek dokusu hasarlarına karşı etkili bir koruma sağladığı belirlenmiştir. Anahtar Sözcükler: Sepsis, Hesperidin, Akciğer, Böbrek, Oksidatif stres, Otofaji

Anahtar Kelimeler

• Sepsis
• Hesperidin
• Akciğer
• Böbrek
• Oksidatif stres
• Otofaji

Kaynakça

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