Investigation of the Cytotoxic Properties of Two Different Soft Lining Materials

Abstract

Objective: The purpose of this study was to examine the cytotoxicity of 2 soft relining materials following 3 different aging periods. Material and Methods: 162 disc specimens of 5 mm radius and 2 mm thickness and made of 2 different soft lining materials Group A: Hydrocast; n=81, Group B: Trusoft; n=81 were divided into 3 subgroups Group I: 1 day water aging, Group II: 2 days water aging , Group III: 7 days water aging . After the water aging periods, the cytotoxicity of the lining materials was determined by using mouse fibroblast L929 cells incubated with 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide after 24 hours, 48 hours and 72 hours. Statistical differences were analysed with ANOVA One-way p

Keywords

• Water aging, Cytotoxicity, Soft relining, 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay

İki Farklı Yumuşak Astar Materyalinin Sitotoksik Özelliklerinin İncelenmesi

Öz

Amaç: Çalışmada, iki farklı yumuşak astar materyalinin suda 3 farklı yaşlandırma süresi sonrasındaki sitotoksik özelliklerinin karşılaştırmalı olarak değerlendirilmesi amaçlanmıştır. Gereç ve Yöntemler: 162 adet 5 mm çapında ve 2 mm kalınlığında 2 farklı yumuşak astar materyalinden Grup A: Hydrocast; n=81, Grup B: Trusoft; n=81 hazırlanan disk örnekler 3 alt gruba ayrıldı. Grup I: 1 gün suda yaşlandırma, Grup II: 2 gün suda yaşlandırma, Grup III: 7 gün suda yaşlandırma. Belirtilen yaşlandırma sürelerini takiben fare fibroblast hücreleri L929 kullanılarak; 24 saat, 48 saat ve 72 saat hücre inkübasyonu sonrasında her grup için ortalama hücre canlılık yüzdeleri 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide testi ile belirlendi. Gruplar arasındaki istatistiksel farklılıkların tespiti için ANOVA One-Way testi kullanıldı. p

Anahtar Kelimeler

• Suda yaşlandırma, Sitotoksisite, Yumuşak astar, 3-[4,5-dimethylthiazol-2- yl]-2,5 diphenyl tetrazolium bromide testi

Kaynakça

1. Akşit KS, Mandalı G, Gürbüz Ö. Protetik tedavide bir yumuşak astar maddesi; Molloplast-B. Atatürk Üniv Diş Hek Fak 2012; 5: 113-22.
2. Lau M, Amarnath GS, Muddugangadhar BC, Swetha MU, Das KA. Tensile and shear bond strength of hard and soft denture relining materials to the conventional heat cured acrylic denture base resin: An In-vitro study. J Int Oral Health 2014;6: 55-61.
3. Kim BJ, Yang HS, Chun MG, Park YJ. Shore hardness and tensile bond strength of long-term soft denture lining materials. J Prosthet Dent 2014;112: 1289-97.
4. Atay A, Saraçlı MA, Akyıl MŞ, Tukay A, Oruç S. Candida Albicans’ in yumuşak astar maddelerine olan adezyonunun modifiye bir teknikle in-vitro değerlendirilmesi. Hacettepe Diş Hek Fak Derg 2007; 31: 74-8.
5. Atay A, Bozok Çetintaş V, Çal E, Kosova B, Kesercioglu A, Guneri P. Cytotoxicity of hard and soft denture lining materials. Dent Mater J 2012; 31: 1082-6.
6. Mancuso DN, Goiato MC, Zuccolotti BCR, Moreno A, dos Santos DM, Pesqueira AA. Effect of thermocycling on hardness, absorption, solubility and colour change of soft liners. Gerodontology 2012; 29: e215-9.
7. Ergün G, Çekiç Nagaş I. In vitro color stability of soft denture liners after accelerated aging. Hacettepe Diş Hek Fak Derg 2007; 31: 65-73.
8. Yerliyurt K, Nalbant D, Kuştimur S. İki farklı akrilik esaslı yumuşak astar maddesinin zamana bağlı olarak yüzeylerinden izole edilen candida albicans hücrelerinde ALS1 adezyon geninin ekspresyonundaki değişimin incelenmesi. Gaziosmanpaşa Üniv Tıp Fak Derg 2012; 4: 7-20.

9. Doğan OM. Yumuşak astar materyallerin klinik endikasyonları. Atatürk Üniv Diş Hek Fak Derg 2005;15: 70-5.
10. Bail M, Jorge JH, Urban VM, Campanha NH. Surface roughness of acrylic and silicone based soft liners: In vivo study in a rat model. J Prosthodont 2014;23: 146-51.
11. Chaves CA, Vergani CE, Thomas D, Young A, Costa CA, Salih VM, Machado AL. Biological effects of soft denture reline materials on L929 cells in vitro. J Tissue Eng 2014;5: 1-8.
12. Tay LY, Herrera DR, Quishida CCC, Carlos IZ, Joge JH. Effect of water storage and heat treatment on the cytotoxicity of soft liners. Gerodontology 2012; 29: 275- 80.
13. Bail M, Meister LMB, Campagnoli EB, Jorge JH, Ban MDCI, Sanchez-Ayala A, Campanha NH. Histopathological changes by the use of soft reline materials: A rat model study. PloS One 2014; 9: e100293.
14. de Andrade Lima Chaves C, Machado AL, Vergani CE, de Souza RF, Giampaolo ET. Cytotoxicity of denture base and hard chairside reline materials: A systematic review. J Prosthet Dent 2012; 107: 114-27.
15. ISO 10993-5: Biological evaluation of medical devices. Part 5: Tests for in vitro cytotoxicity, 2009. http://www. iso.org/iso/catalogue_detail.htm?csnumber=36406. Accessed 27.05.2016.
16. Okuyama Y, Shiraishi T, Yoshida K, Kurogi T, Watanabe I, Murata H. Influence of composition and powder/ liquid ratio on setting characteristics and mechanical properties of autopolymerized hard direct denture reline resins based on methyl methacrylate and ethylene glycol dimethacrylate. Dent Mater J 2014;33: 522-9.
17. Saravi ME, Vojdani M, Bahrani F. Evaluation of cellular toxicity of three denture base acrylic resins. J Dent (Tehran). 2012; 9: 180-8.
18. Neves CB, Lopes LP, Ferrão HF, Miranda JP, Castro MF, Bettencourt AF. Ethanol postpolymerization treatment for improving the biocompatibility of acrylic reline resins. BioMed Res Int 2013; http://dx.doi. org/10.1155/2013/485246 9 pp.
19. Murray PE, Garcia Godoy C, Garcia Godoy F. How is the biocompatibility of dental biomaterials evaluated? Med Oral Patol Oral Cir Buccal 2007; 12: E258–66.
20. Demirci T, Gürbüz T, Şengül F. Dental rezin kompozitlerin sitotoksisitesi: Bir in vitro. Atatürk Üniv Diş Hek Fak Derg 2014;24: 10-5.
21. Bala O, Arısu HD, Türköz E, Yılmaz Ş. Farklı polimerizasyon şekillerinin ormoser esaslı rezin restoratif materyalin sitotoksisitesi üzerine etkisi, in vitro. GÜ Diş Hek Fak Derg 2008; 25: 31-7.
22. Kılıç K, Kesim B, Sümer Z, Polat Z, Öztürk A. Tam seramik materyallerinin biyouyumluluğunun MTT testi ile incelenmesi. Sağlık Bilimleri Derg 2010; 19: 125-32.
23. Chaves CA, Machado AL, Carlos IZ, Giampaolo ET, Pavarina AC, Vergani CE. Cytotoxicity of monomers, plasticizer and degradation by-products released from dental hard chairside reline resins. Dent Mater 2010;26: 1017-23.
24. International Organization for Standardization, ISO7405: Dentistry-Preclinical evaluation of biocompatibility of medical devices used in dentistry- Test methods for dental materials. Geneva: 1997.
25. Ülker HE, Ülker M, Yalçın M, Dündar A. Fissür örtücülerin in vitro sitotoksisitesi. Acta Odontol Turc 2014;31: 7-12.
26. Thonemann B, Schmalz G, Hiller KA, Schweikl H. Responses of L929 mouse fibroblasts, primary and immortalized bovine dental papilla-derived cell lines to dental resin components. Dent Mater 2002;18: 318-23.
27. Sheridan PJ, Ewoldsen NO, Lefebvre CA, Lavin MT. Cytotoxicity of denture base resins. Int J Prosthodont 1997; 10: 73-7.