Rat Alt Ekstremite İskemi Reperfüzyon Hasarının Tenoksikam ile Önlenmesi

Öz

Amaç: Alt ekstremitede uzun süren iskemi/reperfüzyon İ/R lokal dokuda belirgin kas hasarına neden olur. Bu çalışmada alt ekstremite İ/R modelinde bir COX-2 inhibitörü olan tenoksikamın antioksidan etkinliğini araştırmak amaçlanmıştır.Gereç ve Yöntemler: Erkek Wistar ratlarda anestezi verilmesini takiben sol alt ekstremiteye 3 saat iskemi ve 24 saat reperfüzyon uygulandı. Çalışmaya, her birinde 7 denek bulunan 4 grup dâhil edildi. Denekler Grup 1 Kontrol , Grup 2 İ/R, Sham , Grup 3 İ/R, 2,5 mg/kg Tenoksikam ve Grup 4 İ/R, 10 mg/kg Tenoksikam gruplarına ayrıldı. Tenoksikam 3 saat’lik iskeminin ardından hemen intraperitoneal olarak uygulandı. 24 saatlik reperfüzyon dönemi sonunda gastroknemius kasından örnekler alınarak, elektron mikroskopunda incelendi.Bulgular: Elektron mikroskopi ile yapılan çalışmada Grup 2’de, Grup 1’e göre belirgin doku hasarı tespit edildi p=0,005 . Grup 2ʼde yoğun lökosit infiltrasyonu, nekroz, ödem, sarkoplazmik retikulum dilatasyonu, mitokondri kristalarında silinme, miyofibril dezorganizasyonu gözlendi. Grup 3 ve Grup 4ʼde, Grup 2’ye kıyasla özellikle sarkoplazmik retikulum dilatasyonu, mitokondri kristalarında silinme ve miyofibril dezorganizasyonu anlamlı olarak düşük bulundu Sırasıyla p=0,007 ve p=0,006 . Grup 3 ve Grup 4ʼde daha az histopatolojik etkilenme oldu. Tenoksikamın, doza bağlı olarak histolojik değişiklikleri önlediği gözlendi.Sonuç: Alt ekstremite İ/R hasarında belirgin histopatolojik değişiklikler ortaya çıkar. Tenoksikamın kas dokusu iskemi-reperfüzyon hasarını önlemede koruyucu bir etkisi olduğu görülmektedir

Anahtar Kelimeler

• İskemi reperfüzyon, Tenoksikam, Alt ekstremite, Rat, Histopatoloji

Prevention of Rat Lower Extremity Ischemia Reperfusion Injury With Tenoxicam

Öz

Objective: Prolonged ischemia / reperfusion I/R in the lower extremity causes significant muscle damage to the local tissue. In this study, it was aimed to investigate the antioxidant activity of tenoxicam, a COX-2 inhibitor, in rat lower extremity I/R model.Material and Methods: Following anesthesia, 3-hour ischemia and 24-hour reperfusion were applied to the left lower extremity in male Wistar rats. The study included 4 groups, each with 7 subjects. Subjects were divided into Group 1 Control , Group 2 I/R, Sham , Group 3 I/R, 2.5 mg/kg Tenoxicam , and Group 4 I/R, 10 mg/kg Tenoxicam . Tenoxicam was administered intraperitoneally immediately after 3 hours of ischemia. At the end of the 24-hour reperfusion period, samples were taken from the gastrocnemius muscle and examined under an electron microscope.Results: In the study performed with electron microscopy, significant tissue damage was detected in Group 2 compared to Group 1 p=0.005 . In Group 2, intense leukocyte infiltration, necrosis, edema, dilatation of sarcoplasmic reticulum, loss of mitochondrial crystals, and myofibril disorganization were observed. In Group 3 and Group 4, sarcoplasmic reticulum dilatation, disappearance of cristae mitochondria, and myofibril disorganization were significantly lower than in Group 2 p=0.007 and p=0.006, respectively . There was less histopathological involvement in Group 3 and Group 4. Tenoxicam was observed to prevent dose-dependent histological changes.Conclusion: Significant histopathological changes occur in lower extremity I/R injury. Tenoxicam appears to have a protective effect in preventing muscle tissue ischemia-reperfusion injury

Anahtar Kelimeler

• Ischemia-reperfusion, Tenoxicam, Lower extremity, Rat, Histopathology

Kaynakça

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