Yanık Sonrası Destek Tedavisinde N-3 Yağ Asidi Kullanımı Yara İyileşmesini Destekler ve Sistemik Enflamasyonu Engeller. Sıçanlarda Yapılan Deneysel Çalışma

Öz

Amaç: Yanık enfeksiyon, sistemik enflamatuar yanıt sendromu, solunum sıkıntısı sendromu ve çoklu organ yetmezliği yatkınlığını arttırır. Omega-3 (n-3) yağ asitlerinin anti-enflamatuar ve enfeksiyona karşı direnç arttırma etkileri daha önce gösterilmiştir. Ancak yanık sonrası sistemik enflamasyon ve yara iyileşmesi üzerine etkileri bilinmemektedir. Yöntem: Çalışmada 70 Wistar albino sıçan kullanıldı. Yanık olmayan 14 sıçan kontrol grubunu oluşturdu. Geriye kalan 56 sıçanda yanık modeli oluşturuldu ve 50 mg/kg/gün olacak şekilde periton içi ringer laktat solüsyonu eşit iki dozda verildi. Yanığı olan 28 sıçana ayrıca 1 mg/kg/gün dozunda n-3 enjekte edildi. Kontrol grubundaki sıçanlar dördüncü günde, tedavi gruplarındaki sıçanlar dört ve sekizinci günlerde sakrifiye edildi. Histolojik incelemede lökosit ve mast hücre infiltrasyonu, epidermal ve kollajen kalınlık ölçümleri değerlendirildi. Biyokimyasal incelemede beyaz küre, hematokrit, total protein, albumin, fibronektin, TNFα, IL1β ve IL6 seviyeleri ölçüldü. Bulgular: Dördüncü günde n-3 uygulanan grupta lökosit yüksekliği ve mast hücre infiltrasyonu daha düşük, kollajen kalınlığı daha yüksek bulundu. Sekizinci günde n-3 uygulanan grupta lökosit infiltrasyonu açısından fark yok iken mast hücre infiltrasyonu daha düşük ve kollajen kalınlığı daha yüksek bulundu. N-3 uygulanan grupta her iki değerlendirme zamanında da epidermal kalınlık daha yüksekti. Sekizinci günde beyaz küre sayısı dördüncü güne göre daha yüksek bulundu. Sekizinci günde total protein, dördüncü günde IL-6 ve her iki değerlendirme zamanında albumin seviyeleri kontrol grubuna göre daha düşük bulundu. Sonuç: Yanık sonrası n-3 yağ asidi tedavisi yara iyileşmesini destekler ve sistemik enflamasyonu önler.

Anahtar Kelimeler

• beslenme
• enflamasyon
• n-3 yağ asidi
• yanık
• yara iyileşmesi

N-3 Fatty Acid Supplementation After Burn Supports Wound Healing and Prevents Systemic Inflammation. An Experimental Study in Rats

Öz

Objective: Burn may cause hypersensitivity towards infection, systemic inflammatory response syndrome, respiratory distress syndrome and multiple organ failure. Anti-inflammatory and resistance towards infection effects of omega-3 (n-3) fatty acids was shown before. But their effect on systemic inflammation and wound healing after burn was not investigated. Methods: Seventy Wistar albino rats were used in the study. Fourteen rats without burn composed the control group. Cutaneous burn was created on remaining 56 rats which received 50 mg/kg/day intraperitoneal ringer lactat solution in two equal amounts. Twenty-eight rats with burn received injections of n-3 1 mg/kg/day throughout the study. Rats in control group were sacrificed on day 4 and rats in treatment groups were sacrificed on days 4 and 8. Leukocyte and mast cell infiltration, epidermal and collagen thickness measurements were used in histological evaluation. White blood cell, hematocrite, total protein, albumin, fibronectin, TNFα, IL1β and IL6 levels were measured for biochemical evaluations. Results: Elevation of leukocyte and mast cell infiltration was lower and collagen thickness was higher in n-3 receiving group on day 4. There was no difference in leukocyte infiltration on the 8th day whereas mast cell infiltration was lower and collagen thickness was higher in n-3 receiving group. Epidermal thickness was higher in n-3 receiving group on both evaluation days. White blood cell counts were higher in 8th day than 4th day groups. Total protein on 8th, IL-6 on 4th, and albumin levels on both days was lower than control. Conclusion: N-3 fatty acids used after burn enhances wound healing and prevents systemic inflammation.

Anahtar Kelimeler

• nutrition
• inflammation
• n-3 fatty acids
• burn
• wound healing

Kaynakça

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