Rat Testis Torsiyon-Detorsiyon Hasarı Modelinde Silymarinin Antioksidan ve Anti-İnflamatuar Özelliklerinin Değerlendirilmesi

Öz

Amaç: Testis torsiyonu, zamanında müdahale edilmediğinde infertilite ile sonuçlanabilecek ürolojik bir acil durumdur. Bu çalışma, bitki kaynaklı bir flavonoid olan ve antioksidan ile anti-inflamatuar etkileri bilinen silymarinin (SIL), testiküler torsiyon-detorsiyon (T/D) ilişkili iskemi-reperfüzyon (İ/R) hasar rat modelindeki koruyucu rolünü araştırmıştır. Gereç ve Yöntemler: Yirmi dört erkek Wistar albino sıçanı rastgele üç gruba ayrıldı: sham, T/D ve 100 mg/kg SIL. T/D ve SIL gruplarına 2 saat torsiyon, ardından 2 saat detorsiyon uygulandı. Testis doku örneklerinde oksidatif stres belirteçleri (malondialdehit (MDA), total oksidan düzeyi (TOS), miyeloperoksidaz (MPO) ve oksidatif stres indeksi (OSI)), antioksidan aktivite (total antioksidan düzeyi (TAS) ve süperoksit dismutaz (SOD)) ve proinflamatuar sitokinler (interlökin 1-beta (IL-1β) ve tümör nekroz faktör-alfa (TNF-α)) analiz edildi. Bulgular: SIL tedavisi, T/D grubuna kıyasla MDA, TOS, MPO, TNF-α, OSI ve IL-1β düzeylerini anlamlı şekilde azaltırken, SOD ve TAS düzeylerini anlamlı olarak artırdı (p<0.05). Bu da oksidatif ve inflamatuar hasarın azaltıldığını göstermektedir. Sonuç: Elde edilen sonuçlar, SIL’in T/D kaynaklı testiküler hasarda antioksidan ve anti-inflamatuar mekanizmalar yoluyla koruyucu etkiler sağladığını ve doğal bir tedavi adayı olabileceğini göstermektedir.

Anahtar Kelimeler

• Testis torsiyonu-detorsiyonu
• Silymarin
• İskemi-reperfüzyon hasarı
• Rat modeli

Evaluation of Silymarin’s Antioxidant and Anti-Inflammatory Properties in Testicular Torsion-Detorsion Injury Rat Model

Öz

Objective: Testicular torsion represents a urological emergency that, if unaddressed, can culminate in infertility. This study investigated the protective role of silymarin (SIL), a plant-derived flavonoid known for its antioxidant and anti-inflammatory effects, in a rat model of testicular torsion-detorsion (T/D)-associated ischemia-reperfusion (I/R) injury. Materials and Methods: Twenty-four male Wistar albino rats were randomized into three groups: sham, T/D, and 100 mg/kg SIL. The T/D and SIL groups underwent 2 hours of torsion followed by 2 hours of detorsion. Testicular tissue samples were analyzed for markers of oxidative stress (malondialdehyde (MDA), total oxidant status (TOS), myeloperoxidase (MPO), and oxidative stress index (OSI)), antioxidant activity (total antioxidant status (TAS) and (superoxide dismutase (SOD)), and proinflammatory cytokines (interleukin 1-beta (IL-1β and tumor necrosis factor- alpha (TNF-α)). Results: SIL treatment significantly diminished MDA, TOS, MPO, TNF-α, OSI, and IL-1β, and increased SOD and TAS in comparison to the T/D group (p<0.05), implying a mitigation of oxidative and inflammatory damage. Conclusion: The findings indicate that SIL confers protective benefits in T/D-induced testicular injury through its antioxidant and anti-inflammatory mechanisms, making it a promising natural candidate.

Anahtar Kelimeler

• Testicular torsion-detorsion
• Silymarin
• Ischemia-reperfusion injury
• Rat model

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