Klinik Kullanım İçin Mezenkimal Kök Hücrelerin Hazırlanması

Yıl 2019, Cilt: 5 Sayı: 2, 169 - 175, 01.01.2019

Zeynep Burçin Gönen Nur Seda Şahin

Öz

Mezenkimal kök hücreler MKH kendini yenileme potansiyeli olan, osteoblast, kondrosit ve adiposit gibi özelleşmiş hücrelere farklılaşabilme yeteneğine sahip, erişkin, multipotent hücrelerdir. Klinikte kullanılmaları için özel üretim koşulları gerekmektedir. GMP Good Manufacturing Practice- İyi Üretim Uygulamaları şartlarında hücre üretimi, temel kök hücre bilimlerinden klinik araştırmalara ve uygulamalara translasyon için yüksek bir kalite güvence sistemi sağlar. Üretim protokolünde kullanılan enzim içeriği ve konsantrasyonu, parçalanma süresi, santrifüj hız farklılıkları, filtre boyutu gibi değişkenler, MKH eldesindeki farklılıkları oluşturur. Uygun üretim, doz ve uygulama yolu standardize edilerek hastada en başarılı tedavi sonucuna ulaşılır. Derlemede, MKH' lerin özelliklerini ve klinikte kullanımlarına özgü olarak nasıl hazırlandıklarını ve hazırlanma esnasındaki değişkenlerin hücre üzerine etkilerini açıklanması amaçlanmıştır

Anahtar Kelimeler

Mezenkimal kök hücre, Translasyonel tıp, GMP

Mesenchymal Stem Cell Production for Clinical Usage

Öz

Mesenchymal stem cells MSC are multipotent cells that can differentiate into specialized cells such as osteoblasts, chondrocytes and adipocytes, and have the ability for self-renewal. However, special production conditions are required for clinical usage. GMP Good Manufacturing Practice Laboratory conditions may provide a high quality control system in translational medicine from basic stem cell research to clinical trials. The content and concentration of the enzyme, digestion period, the speed of the centrifuge and filter size may be among the variables that affect the characteristics of MSCs. The production methods, application and dose of MSCs for certain therapies should therefore be standardized according to the best clinical treatment available for the patient. The aim of this review is to explain the characteristics of MSCs, the special production methods for their use in clinical practice, and their effects on various cells

Anahtar Kelimeler

Mesenchymal stem cell, Translational medicine, GMP

Kaynakça

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