GHSR rs2232165 Gen Polimorfizminin Genotiplendirilmesi için PCR-RFLP Yönteminin Optimizasyonu ve Türk Erkeklerinde Alel Frekansının Belirlenmesi

Yıl 2022, , 1245 - 1255, 28.12.2022

Selin Özkan Kotiloğlu , Dilek Kaya Akyüzlü , Gizem Düzgün , Mustafa Danışman , Ece Ağtaş Ertan , İnci Özgür İlhan

https://doi.org/10.35414/akufemubid.1137753

Öz

Çağımızın ciddi sorunlardan biri olan alkol bağımlılığı, genetik, çevresel, kültürel, gelişimsel ve nörobiyolojik faktörlerin etkisiyle ortaya çıkmaktadır. Son yıllarda yeme davranışı nörobiyolojisi ile bağımlılık yapan maddelere aşerme davranışının nörobiyolojisinin benzer özelliklere sahip olduğunun keşfedilmesi araştırmacıları, alkol bağımlılığı ile grelin gibi besin alımında önemli olan hormonlar arasındaki ilişkiyi araştırmaya sevk etmiştir. Alkol kullanım bozukluğunda ve alkole aşermede grelin sisteminin önemi mevcut çalışmalar tarafından ortaya konulmasına karşın bu ilişkinin biyolojik mekanizmasının aydınlatılması için daha çok çalışmaya ihtiyaç vardır. Bu nedenle, bu çalışmada, alkol kullanım sorunu olan Türk erkeklerinde, grelin reseptörünü kodlayan GHSR genindeki rs2232165 polimorfizminin alkol kullanım sorunu ile ilişkisi araştırılmıştır. Çalışmaya alkol kullanım bozukluğu tanısı konmuş 72 erkek birey ile herhangi bir madde bağımlılığı olmayan 82 sağlıklı erkek dahil edilmiştir. GHSR rs2232165 gen polimorfizmi, ilk defa bu çalışmada optimize edilen PCR-RFLP yöntemi ile genotiplendirilmiştir. GHSR rs2232165 polimorfizmi için alel frekansları alkol kullanım sorunu olan grupta (n:144); C aleli için 0,99 (n:142), T aleli için 0,01 (n:2) olarak hesaplanmıştır. Karşılaştırma grubunda ise (n:164), C aleli frekansı 0,98 (n:161), T aleli frekansı 0,02 (n:3) olarak belirlenmiştir. İki grup arasında, T alel frekansı açısından istatistiksel olarak anlamlı bir fark bulunmamıştır (p>0,05). Gen polimorfizmleri, çevresel faktörlerden etkilenmediği için Türk erkeklerindeki minör alel frekansını (MAF) belirlemek için iki gruptaki bireyler birleştirilmiş (n=154) ve Türk erkeklerinde GHSR rs2232165 polimorfizminin MAF değeri 0,02 olarak belirlenmiştir. Bu çalışmada, çağımızda alkol kullanım bozukluğu kadar önemli olan obezite etiyolojisinde de rol oynayan GHSR rs2232165 polimorfizminin Türk erkeklerinde alel frekansı ile ilgili ilk veriler toplanmıştır.

Anahtar Kelimeler

Alkol Kullanım Bozukluğu, Grelin, Gen Polimorfizmi, GHSR rs2232165, PCR-RFLP

Destekleyen Kurum

Ankara Üniversitesi

Proje Numarası

20B0217001

Teşekkür

Bu çalışma Ankara Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğü tarafından 20B0217001 No.’lu proje kapsamında desteklenmiştir.

Optimization of PCR-RFLP Method for Genotyping of GHSR rs2232165 Gene Polymorphism and Determination of Allele Frequency in Turkish men

Öz

Alcohol addiction, which is one of the crucial problems of the current era, arises with the effects of genetic, environmental, culturel, developmental and neurobiological factors. In recent years, the discovery that the neurobiology of eating behavior and the neurobiology of craving for addictive substances have similar characteristics has prompted researchers to investigate the relationship between alcohol addiction and hormones important in food intake such as ghrelin. Even though the importance of the ghrelin system in alcohol use disorder and alcohol craving has been stated by existing studies , more studies are needed to enlighten the biological mechanism of this relationship. Therefore, in this study the relationship between rs2232165 polymorphism in GHSR gene encoding ghrelin receptor and alcohol use disorder in Turkish men with alcohol use problem. Seventy-two individuals diagnosed with alcohol use disorder and 82 healthy men without any substance addiction were included in this study. The GHSR rs2232165 gene polymorphism was genotyped by PCR-RFLP which was optimized for the first time in this study. The allele frequency of the GHSR rs2232165 gene polymorphism was determined in the group with alcohol use problem (n:144) as 0,99 (n:142) for C allele and 0,01 (n:2) for T allele. The frequency of the C allele was 0,98 (n:161) and T allele was 0,02 (n:3) in the healthy group. There was no statistically significant difference between two groups in terms of T allele frequency. Since gene polymorphisms are not affected by environmental factors, individuals in two groups were combined (n=154) in order to determine minor allele frequency (MAF) in Turkish males, and the MAF value of GHSR rs2232165 polymorphism in Turkish males was determined as 0,02. In this study, the first data on the allele frequency of GHSR rs2232165 polymorphism, which plays a role in the etiology of obesity, which is as important as alcohol use disorder in the current era, were collected in Turkish males.

Anahtar Kelimeler

Alcohol Use Disorder, Ghrelin, Gene Polymorphism, GHSR rs2232165, PCR-RFLP

Proje Numarası

20B0217001

Kaynakça

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