Ovicidal Effect of Beauveria bassiana Vuillemin Isolates on Tetranychus urticae Koch (Acari: Tetranychidae)

Yıl 2024, , 485 - 491, 27.06.2024

Mert Yılmaz Uysal Melike Obut Asiye Uzun Yiğit

https://doi.org/10.35414/akufemubid.1384515

Öz

This study aimed to determine the ovicidal effect of three different doses (1x106 spore/ml, 1x107 spore/ml and 1x108 spore/ml) of Beauveria bassiana isolates BY2 (Burdur, Yeşilova), BIM-001 (Isparta, Center) and IGÇ (Isparta, Center) on Tetranychus urticae under laboratory conditions. The commercial preparation Nostalgist (Agrobest Company, Beauveria bassiana strain Bb-1) and water containing Tween 80 (0,1%) were used as control. Experiments were carried out in plastic petri dishes (9 cm) with 5 replications. Bean leaf disks (4 cm) were placed on sterile water-saturated cotton held in a Petri plate. Then, 10 adult individuals were transferred to leaf discs and allowed to lay eggs. Each petri plate was set to contain 20 eggs/leaf discs. The spore suspension of each entomopathogenic fungus isolate was applied to the leaf discs containing eggs for 10 seconds with the help of a modified device that provides spraying at 4 bar pressure. Observations started 24 hours after the application and egg deaths were recorded. As a result, 7 days after application of 1x108 spore/ml concentrations of B. bassiana BIM-001, BY2, and IGÇ isolates, the mortality rates in T. urticae eggs were determined as 71%, 66%, and 59%, respectively. Application of B. bassiana strain Bb-1 was found to be statistically significant compared to applications of other entomopathogenic fungus isolates. However, it was determined that there was no difference between the mortality rates in B. bassiana BIM-001 1x108, 1x107 spore/ml and B. bassiana BY2 1x108 spore/ml applications (p˂0.005).

Anahtar Kelimeler

Microbial control, Entomopathogenic fungus, Spore suspension, Two spotted spider mite

Proje Numarası

TBTK-0117-0114

Beauveria bassiana Vuillemin İzolatlarının Tetranychus urticae Koch (Acari: Tetranychidae) Üzerine Ovisidal Etkisi

Öz

Bu çalışma ile Beauveria bassiana’nın BY2 (Burdur, Yeşilova), BIM-001 (Isparta, Merkez) ve IGÇ (Isparta, Merkez) izolatlarının üç farklı dozunun (1x106 spor/ml, 1x107 spor/ml ve 1x108 spor/ml) Tetranychus urticae üzerinde ovisidal etkisinin laboratuvar koşullarında belirlenmesi hedeflenmiştir. Ticari preparat Nostalgist (Agrobest Grup Ltd. Şti, Beauveria bassiana Bb-1 ırkı) ve kontrol olarak Tween 80 (% 0,1) içeren saf su kullanılmıştır. Denemeler plastik petrilerde (9 cm) 5 tekerrürlü olarak yürütülmüştür. Fasulye yaprak diskleri (4 cm) petri plakasında tutulan steril suya doymuş pamuk üzerinde yerleştirilmiştir. Daha sonra 10 ergin birey, yaprak disklere aktarılarak yumurta bırakmalarına izin verilmiştir. Her bir petri plakasında 20 yumurta/yaprak diski olacak şekilde ayarlanmıştır. Her bir entomopatojen fungus izolatına ait spor süspansiyonu 4 bar basınçta püskürtme sağlayan modifiye edilmiş bir düzenek yardımıyla 10 sn süre ile yumurta içeren yaprak disklerin üzerine uygulanmıştır. Gözlemlere uygulamadan 24 saat sonra başlanarak yumurta ölümleri kaydedilmiştir. Sonuç olarak, B. bassiana BIM-001, BY2 ve IGÇ izolatlarının 1x108 spor/ml konsantrasyonları uygulandıktan 7 gün sonra T. urticae yumurtalarında meydana gelen ölüm oranları sırasıyla %71, %66 ve %59 olarak belirlenmiştir. Beauveria bassiana strain Bb-1 uygulaması diğer entomopatojen fungus izolatlarına ait uygulamalardan istatistiksel olarak anlamlı bulunmuştur. Bununla birlikte B. bassiana BIM-001 1x108, 1x107 spor/ml ve B. bassiana BY2 1x108 spor/ml uygulamalarındaki ölüm oranları arasında fark olmadığı saptanmıştır (p˂0.005).

Anahtar Kelimeler

Mikrobiyal mücadele, Entomopatojen fungus, Spor süspansiyonu, İki noktalı kırmızıörümcek

Destekleyen Kurum

TÜBİTAK 2209-A Üniversite Ögrencileri Arastırma Projeleri Destekleme Programı

Proje Numarası

TBTK-0117-0114

Teşekkür

2209-A Üniversite Öğrencileri Araştırma Projeleri Destekleme Programı kapsamında çalışmayı desteklediği için TÜBİTAK Bilim İnsanı Destek Programları Başkanlığı (BİDEB)’na teşekkür ederiz.

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