Kükürt İçeren Ligand ve Bakır Kompleksine Ait Antioksidan ve Topoizomeraz I İnhibitör Aktivitelerin Karşılaştırılması

Yıl 2020, , 30 - 34, 17.03.2020

Ufuk Yıldız

https://doi.org/10.35414/akufemubid.629432

Öz

Bu çalışmada antikanser
ve antioksidan ajan olarak kullanılabilecek dtb ligandı ve bu ligantın
[Cu(dtb)(phen)]²⁺ kompleksi ile çalışılmıştır. Antioksidan
aktivitelerinin belirlenmesi için DPPH radikali süpürme yöntemi kullanılmıştır.
Dtb ligandı düşük antioksidan aktivite gösterirken bu ligantın Cu(II)
kompleksinin iyi bilinen bir antioksidan olan C vitamini kadar yüksek
antioksidan aktivite gösterdiği görülmüştür. Topoizomeraz-I inhibisyonu
aktivitesi çalışmasında pBR322 plazmid DNA’sının relaksasyonu agaroz jel
elektroforez yöntemiyle takip edilmiştir. Antioksidan aktivitede olduğu gibi
topoizomeraz-I inhibisyonu aktivitesinde de dtb ligandı düşük etki gösterirken
bakır kompleksi µM gibi düşük derişimde bile inhibisyon gerçekleştirebilmiştir.
[Cu(dtb)(phen)]²⁺ kompleksinin antikanser ve antioksidan ajan olarak
kullanılabilme potansiyelinin yüksek olduğu görülmüştür. 

Anahtar Kelimeler

Antioksidan, Topo-I, Cu(II), Benzimidazol

Destekleyen Kurum

ZONGULDAK BÜLENT ECEVİT ÜNİVERSİTESİ

Comparison of Antioxidant and Topoisomerase I Inhibitor Activities of Sulfur Containing Ligand and Copper Complex

Öz

In this study, dtb ligand and its [Cu (dtb) (phen)]2+ complex which can be used as anticancer and antioxidant agent were studied. DPPH radical scavenging method was used to determine the antioxidant activities. The dtb ligand had low antioxidant activity, while the Cu (II) complex of this ligand had as high antioxidant activity as vitamin C, a well-known antioxidant. In the topoisomerase-I inhibition activity study, relaxation of pBR322 plasmid DNA was followed by agarose gel electrophoresis method. In addition to antioxidant activity, dtb ligand had a low effect on topoisomerase-I inhibition activity, while the copper complex was able to inhibit even at low concentrations such as 10 µM. The obtained results show [Cu (dtb) (phen)]2+ complex has a high potential to be used as an anticancer and antioxidant agent.

Anahtar Kelimeler

Antioxidant; Topo-I; Cu(II); Benzimidazole

Kaynakça

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