Vigna caracalla L. Verdc. Bitkisinde In Vitro Klonal Mikroçoğaltım

Yıl 2020, Cilt: 20 Sayı: 4, 753 - 767, 25.09.2020

Halide Hande Güngör , Begüm Güler , Meltem Bayraktar , Aynur Gürel

https://doi.org/10.35414/akufemubid.634363

Cited By: 1

Öz

Vigna caracalla L. Verdc., “İzmir sarmaşığı’’ olarak da adlandırılan, hoş kokulu güzel çiçekleriyle dikkat çeken bir süs bitkisidir. Bu çalışmada, Vigna caracalla L. Verdc. bitkisinde in vitro klonal mikroçoğaltımın gerçekleştirilmesi için etkili bir protokolün geliştirilmesi amaçlanmıştır. Tohumlar, farklı sterilizasyon yöntemlerine maruz bırakıldıktan sonra MS besin ortamlarında kültüre alınmışlar. En yüksek sterilizasyon (%93.33) oranı (%93.33); 1 dk %70 etil alkol, 4 dk %0.1 HgCl2 uygulamasının ardından, 7 saat suda bekletilen tohumlarda elde edilmiştir. En yüksek çimlenme yüzdesi (%95.24), N6 besin ortamı içeren 250 mL’lik erlenlerde gözlenmiştir. En yüksek ortalama sürgün uzunluğu (5.05 cm) 1 mg/L IBA + 0.5 mg/L BAP içeren MS besin ortamında kültüre alınan sürgün ucu eksplantlarından elde edilmiştir. Jelleştirici ajan, ışık şiddeti ve eksplant tipinin sürgün rejenerasyonuna etkisinin belirlenmesi için yapılan denemelerde ise en yüksek çoğaltım katsayısı (1.71) ve yaprak boyu (0.82 cm), Duchefa agar ile katılaştırılmış N6 besin ortamında 4200 lüks ışık şiddetine maruz bırakılan nod eksplantlarından elde edilmiştir. Bu çalışma kapsamında, fazla hiperhidrisite göstermeyen ve çoğaltım oranı daha yüksek olan 16 adet klonun mikroçoğaltım denemelerinde uygun reaksiyon verdiği belirlenmiştir. En yüksek kök rejenerasyonu (%85.71), Fluka agar ile katılaştırılmış N6 besin ortamındaki 4200 lüks ışık şiddetine maruz bırakılan sürgün ucu eksplantlarında saptanmıştır. Köklü sürgünler, %70 başarı yüzdesi ile aklimatize edilmişlerdir.

Anahtar Kelimeler

Vigna caracalla L. Verdc., in vitro, klonal mikroçoğaltım, çoğaltım katsayısı

Destekleyen Kurum

Aliye Üster Vakfı

Teşekkür

Bu çalışmayı ''Selluka-İzmir Sarmaşığı (Vigna caracalla L. verdc.) Bitkisinin In vitro Klonal Mikroçoğaltımı' başlıklı proje olarak Eylül 2017- Eylül 2018 tarihleri arasında destekleyen ALİYE ÜSTER VAKFI’na teşekkür ederiz.

In Vitro Clonal Micropropagation of Vigna caracalla L. Verdc.

Öz

Vigna caracalla L. Verdc. is an ornamental plant, having with its beautiful odorous remarkable flowers, named as "İzmir's ivy". In this study, it was aimed to develop an effective protocol for in vitro clonal micropropagation in Vigna caracalla L. Verdc. plant. The seeds were cultured in MS medium after exposed to different sterilization methods. The highest percentage of sterilization (93.33%) were achieved when the seeds were soaked in 70% ethyl alcohol for 1 min, 0.1% HgCl2 for 4 min and then kept sterilized distilled water for 7 hours. The highest percentage of germination (95.24%) was observed in 250 mL Erlenmeyer flasks containing N6 medium. The highest shoot length (5.05 cm) was obtained from shoot tip explants cultured in MS medium containing 1 mg/L IBA + 0.5 mg/L BAP. In the experiments carried out to determine the effect of gelling agent, light intensity and explant type on shoot regeneration, the highest multiplication coefficient (1.71) and leaf length (0.82 cm) was achieved in node explants cultured in N6 medium solidified with Duchefa agar and exposed to 4200 lux light intensity. Within the scope of this study, it was determined that 16 clones that did not show much hyperhydricity and had higher multiplication rate gave appropriate responses for micropropagation experiments. The highest root regeneration (85.71%) was achieved from shoot tip explants cultured in N6 medium solidified with Fluka agar and exposed to 4200 lux light intensity. The rooted plantlets were acclimatized with 70% success.

Anahtar Kelimeler

Vigna caracalla L. Verdc., In vitro, Clonal micropropagation, Multiplication coefficient

Kaynakça

• Aasim, M, Khawar KM, Özcan S, 2008. In vitro micropropagation from shoot meristems of Turkish cowpea (Vigna unguiculata L.) cv. Akkız. Bangladesh Journal of Botany, 37(2), 149-154.
• Aasim, M, Day, S, Rezaei, F, Hajyzadeh, M, Mahmud, ST, Ozcan, S, 2011. In vitro shoot regeneration from preconditioned explants of chickpea (Cicer arietinum L.) cv. Gokce. African Journal of Biotechnology, 10(11), 2020-2023.
• Adlinge, PM, Samal, KC, Kumara Swamy, RV, Ranjan Rout, GR, 2014. Rapid in vitro plant regeneration of black gram (Vigna mungo L. Hepper) var. sarala, an important legume crop. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences, 84(3), 823–827.
• Ahmed, T, Sharif Uddin Imam, KM, Rahman, S, Mou, SM, Choudhury, MS, Mahal, MJ, Jahan, S, Hossain, MS, Rahmatullah, M, 2012. Antihyperglycemic and antinociceptive activity of Fabaceae family plants – an evaluation of Mimosa pigra L. Stems. Advances in Natural and Applied Sciences, 6(8), 1490-1495.
• Anderson, NO, Asche, PD, 1994. Breakup of linkages for dıagnostıc traıts ın three-specıes congruıty backcross (cbc) Phaseolus hybrıds. Journal of the American Society for Horticultural Science, 29(5), 496.
• Chu, CC, Wang, CC, Sun, CS, Msu, C, Yin, KC, Chu, CY, Bi, CY, 1975. Establishment of an efficient medium for anther cultures of rice through comparative experiments on nitrogen sources. Scientia Sinica, 18, 659-668.
• Etcheverry, AV, Aleman, MM, Fleming, TF, 2008. Flower Morphology, Pollination Biology and Mating System of the Complex Flower of Vigna caracalla (Fabaceae: Papilionoideae). Annals of Botany, 102: 305–316.
• Chanda, S, Dudhatra, S, Kaneria, M, 2010. Antioxidative and antibacterial effects of seeds and fruit rind of nutraceutical plants belobging to the Fabeceae family. Food&Funciton, 1, 308–315.
• Dewir, YH, Murthy, HN, Ammar, MH, Alghamdi, SS, Al-Suhaibani, NA, Alsadon, AA, Paek, KY, 2016. In vitro Rooting of Leguminous Plants: Difficulties, Alternatives, and Strategies for Improvement. Horticulture, Environment, and Biotechnology, 57(4), 311-322.
• Diallo, MS, Ndiaye, A, Sagna, M, Gassama-Dia, YK, 2008. Plant regeneration from African cowpea variety (Vigna ungiculata L. Walp.) African Journal of Biotechnology, 7(16):2828-2833.
• Gulati, A, Jaiwal, PK, 1992. In vitro induction of multiple shoots and plant regeneration from shoot tips of mung bean (Vigna radiata (L.) Wilczeck). Plant Cell, Tissue and Organ Culture, 29, 199-205.
• Gulati, A, Jaiwal, PK, 1994. Plant regeneration from cotyledonary node explants of mungbean (Vigna Radiata (L.) Wilczek). Plant Cell Reports, 13:523-527.
• Lloyd, G, McCown, BH, 1980. Commercially feasible micropropagation of mountain laurel, (Kalmia latifolia) by use of shoot tip culture. Combined Proceedings of International Plant Propagators’ Society, 30, 421-427.
• Mahbubur Rahman, AHM, Ismot Ara Parvin, M, 2014. Study of Medicinal Uses on Fabaceae Family at Rajshahi, Bangladesh. Research in Plant Sciences, 2(1), 6-8.
• Molares, S, Ladio, A, 2011. The Usefulness of Edible and Medicinal Fabaceae in Argentine and Chilean Patagonia: Environmental Availability and Other Sources of Supply. Evidence-Based Complementary and Alternative Medicine, 2012,1-12.
• Murashige, T, Skoog, F,1962. A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiologia Plantarum, 15, 473-497.
• Rao S, Patil, P, 2012. Biotechnology - Molecular Studies and Novel Applications for Improved Quality of Human Life. Cilt no, Reda Sammour, Intechopen, 197-212.
• Saha, P, Afrin, M, Mohiuddin, AKM, Shohae, AM, 2017. Development of an effective in vitro Regeneration protocol for BARI Mash 2 (Vigna mungo L.) an important legume crop in Bangladesh. Jahangirnagar University Journal of Biological Sciences, 6(1), 23-33.
• Silué, N, Koné, T, Soumahoro, AB, Kon, M, 2016. In vitro shoot tip multiplication of bambara groundnut [Vigna subterranea (L.) Verdc.]. Plant Cell Tissue and Organ Culture, 127, 603-611.
• Takahashi, Y, Somta, P, Muto, C, Iseki, K, Naito, K, Pandiyan, M, Natesan, S, Tomooka, N, 2016. Novel Genetic Resources in the Genus Vigna Unveiled from Gene Bank Accessions. Plos One, 11(1), 1-18.
• Thorat, BS, Patil, RR, Kamble, AR, 2017. Effect of growth regulators on germination and vigour of cow pea (Vigna unguiculata L. Walp.) seeds. International Journal of Chemical Studies, 5(6), 766-769.
• Suleiman MK, 2003. Seed germination of ornamental plants: a greenery plan contribution. Archives of Agronomy and Soil Science, 49, 37–44.
• http://www.tolweb.org/Fabaceae (14.10.2019)
• https://www.kadirbekci53.blogspot.com/2013/09 (14.10.2019)
• https://www.missouribotanicalgarden.org/PlantFinder/PlantFinderDetails.aspx?taxonid=280333&isprofile=1&basic=Vigna%20caracalla (14.10.2019)
• https://izmirobm.ogm.gov.tr/SitePages/OGM/OGMBiliyormuydunuz.aspx?l=64cb0954-5394-4fcc-83e9-dc545a01a899&i=1 (14.10.2019)
• https://www.tohumdunyasi.com.tr/selluka-tohumu-izmir-sarmasigi-muhtesem-kokulu-cok-nadir-bitki-turu (14.10.2019)
• https://plants.usda.gov/core/profile?symbol=VICA83 (17.10.2019)