Synthesis and Characterization of TiO2, Ag and TiO2@Ag Nanoparticles via Assessing the Effects on Stem Cells in vitro

Yıl 2022, Cilt: 22 Sayı: 3, 454 - 464, 30.06.2022

Serap Yeşilkır Baydar Melahat Bağırova Adil Allahverdiyev Emrah Şefik Abamor

https://doi.org/10.35414/akufemubid.1030781

Öz

Stem cells and nanotechnology are two of the most talented research areas and a new area called stem cell nanotechnology has emerged. With knowledge of nanoparticles’s inter- and intra-cellular transport abilities, developments on stem cell nanotechnology increases. The aim of this study is to investigate the effects of different featured nanoparticles silver, titaniumdioxide and titaniumdioxide-silver combination on human adipose tissue derived mesenchymal stem cells (ADMSCs), for the first time. Cell culture experiments and characterization parameters were practiced for ADMSCs. Moreover, nanoparticles were synthesized, Zeta and SEM characterization protocols were performed. MTT assay was performed for cytotoxicity. Cells were differentiated into adipocytes and osteocytes. The effects of nanoparticles on ADMSC differentiation were studied by staining protocols. Results showed effects of nanoparticles differ from each other. TiO2 NPs were non-toxic up to 20 μg/mL. Ag NPs have a significant proliferative effect at 1 μg/mL. TiO2@Ag NPs showed a high proliferative effect at all concentrations that applied. Evaluation of TiO2@Ag NPs particularly showed that, effects of combination of TiO2 and Ag NPs are different for same cell line. Results also support further investigation of nanoparticles solely and combinations on stem cell viability, cellular functions and cell fates, and also advantages for tissue engineering as an extra cellular matrix unit.

Anahtar Kelimeler

Nanoparticle synthesis, TiO2@Ag, Scanning electron microscopy, Stem cells, Tissue engineering

Destekleyen Kurum

The authors gratefully acknowledge the Scientific Research Council of Yildiz Technical University

Proje Numarası

Project No: 2011-07-04-KAP06

Teşekkür

We thank to Prof. Dr. Cengiz Kaya and Prof. Dr. Figen Kaya for their nanoparticle support for this study.

TiO2, Ag ve TiO2@Ag Nanopartiküllerinin Sentezi, Karakterizasyonu ve Kök Hücreler Üzerindeki Etkilerinin in vitro Değerlendirilmesi

Öz

Kök hücre ve nanoteknoloji son yılların en hızlı gelişen araştırma alanlarındandır ve bu iki önemli alanın birleşmesi ile kök hücre nanoteknolojisi adı verilen yeni bir branş ortaya çıkmıştır. Yapılan çalışmalar ile nanoparçacıkların hücre içine girebildiği ve hücreler arası taşınabildiğinin belirlenmesinin ardından kök hücre nanoteknolojisindeki gelişmelerin arttığı görülmektedir. Bu çalışmanın amacı, ilk kez olarak, farklı karakteristik özelliklere sahip olduğu bilinen gümüş ve titanyumdioksit nanopartikülleri ile bu nanopartiküllerin kombinasyonu ile elde edilen titanyumdioksit-gümüş nanopartikülünün insan yağ dokusu kaynaklı mezenkimal kök hücreler (hADMKH'ler) üzerindeki etkilerini araştırmaktır. hADMKH'ler hücre kültürü yapılarak çoğaltılmıştır. Diğer taraftan nanopartiküller sentezlenmiş, elde edilen nanopartiküller için Zeta potansiyeli tayin edilip SEM ile görüntüleme yapılmıştır. Üç farklı nanopartikül türünün farklı konsantrasyonlarının hücreler üzerindeki toksisitesi MTT testi ile belirlenmiştir. Ayrıca nanopartiküllere maruz bırakılan hücrelerin adipojenik ve osteojenik farklılaşma potansiyelleri Oil Red O ve Alizarin Red S boyama ile incelenmiştir. Elde edilen sonuçlar aynı hücre hattı üzerinde her bir nanopartikül türünün farklı konsantrasyonlarının etkilerinin birbirinden farklı olduğunu göstermiştir. TiO2 nanopartikülleri 20 μg/mL'ye kadar toksik değilken Ag nanopartiküllerinin 1 μg/mL'de hücreler üzerinde önemli bir proliferatif etkisi vardır. TiO2@Ag nanopartikülleri ise tüm konsantrasyonlarda proliferatif etkide artış göstermiştir. Sonuç olarak tek başına kullanılan nanopartiküllerin hücreler üzerinde gösterdikleri etkilerinin yanı sıra nanopartikül kombinasyonlarının da ayrıca incelenmesine ve nanopartiküllerin kök hücre canlılığı, hücresel fonksiyonlar üzerindeki etkileri ve hücrelerin akıbeti üzerinde araştırmalar yapılmasına ihtiyaç duyulmaktadır. Ayrıca nanopartiküllerin ve kombinasyonlarının özellikle doku mühendisliği uygulamaları için hücre dışı bir matris elemanı olarak etkilerinin belirlenmesine yönelik çalışmaların artırılması gerekliliği ortaya çıkmaktadır.

Anahtar Kelimeler

Nanopartikül sentezi, TiO2@Ag, Taramalı elektron mikroskopisi, Kök hücre, Doku mühendisliği

Proje Numarası

Project No: 2011-07-04-KAP06

Kaynakça

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