Karate Zeon Pestisitinin Mutajenik Aktivitesinin Ames Testi ile Değerlendirilmesi

Yıl 2022, Cilt: 22 Sayı: 3, 465 - 469, 30.06.2022

Arzu Özkara

https://doi.org/10.35414/akufemubid.1069842

Cited By: 1

Öz

Pestisitler, ürünlerin kalitesi ve veriminin artmasında tarımda yaygın olarak kullanılırlar. Ancak pestisitler, hedef olmayan organizmalar üzerinde pestisit direnci, mutajenik ve karsinojenik etkilerinden dolayı çevre için büyük bir endişe kaynağı haline gelmiştir. Bu çalışmada, sentetik bir piretroid insektisit olan Karate Zeon'un potansiyel mutajenitesinin araştırılması amaçlanmıştır. Salmonella typhimurium mutajenite testi (Ames testi), S9 fraksiyonunun varlığı ve yokluğunda Karate Zeon insektisitinin iki suş (TA98 ve TA100) üzerindeki mutajenitesini değerlendirmek için kullanılmıştır. Bu çalışmada, Karate zeonun 250, 25, 2.5, 0.25, 0.025 µg/plak dozları kullanılmıştır. Karate Zeon pestisiti, hem TA98 hem de TA100 üzerinde metabolik aktivasyon varlığı ya da yokluğunda 250 μg/plak konsantrasyonunda mutajenik aktivite göstermiştir.

Anahtar Kelimeler

Ames Testi, Karate zeon, Mutajenite, Pestisit

Assessment of Mutagenic Activity of Karate Zeon Pesticide by Ames Test

Öz

Pesticides widely used in the agricultural market led to an increase in the quality and yield of crops. However, pesticides are a major concern in the environment as they also harm non-target creatures, because of pesticide resistance, and mutagenic and carcinogenic effects. In this study, we aimed to research the potential mutagenicity of Karate Zeon which is a synthetic pyrethroid insecticide. Salmonella typhimurium mutagenicity assay (Ames test) was used to evaluate the mutagenicity of Karate Zeon insecticide in the absence and presence of the S9 fraction on two strains (TA98 and TA100). In this study, doses of 250, 25, 2.5, 0.25, 0.025 μg/plate of Karate Zeon were used. Karate Zeon pesticide showed mutagenic activity at 250 μg/plate concentration on both TA98 and TA100 with and without metabolic activation.

Anahtar Kelimeler

Ames test, Karate zeon, Mutagenicity, Pesticide

Kaynakça

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