Tereftalik Asit Parçalayan Pseudomonas sp.’nin Depolanması için Liyofilizasyon ve Saklama Teknikleri: Prosedür ve Koruyucu Ajanlar

Yıl 2025, Cilt: 25 Sayı: 5, 1008 - 1017, 01.10.2025

Basri Aksu , Didem Aksu , Burçin Karabey , Güven Özdemir

https://doi.org/10.35414/akufemubid.1581597

Öz

Endüstriyel atıkların kontrolsüz bir şekilde doğaya salınması, çevre kirliliğini artırarak ekosistem dengesini tehdit eden ciddi sorunlara yol açmaktadır. Tereftalik asit (TA), polietilen tereftalat (PET) üretiminde temel bir bileşen olup, plastik şişeler, poliester lifler ve ambalaj malzemeleri gibi birçok endüstriyel uygulamada kullanılmaktadır. TA'nın atık su sistemlerinden etkin bir şekilde uzaklaştırılması, çeşitli biyoteknolojik yöntemlerle mümkün olmaktadır. Bu bağlamda, arıtım bakterileri, atık su yönetiminde sürdürülebilir çözümler sunarak çevre koruma açısından kritik bir rol oynamaktadır. Bu çalışmanın amacı, Pseudomonas sp. türünün farklı koruyucu ve liyofilizasyon yöntemleri ile belirli sürelerde saklanmasının biyodegradasyon aktivitesine ve bakteri canlılığına olan etkilerini incelemektir. Çalışmada, saklama koşullarını iyileştirmek amacıyla süt tozu, sakaroz, silika jel ve ortam sıvısı gibi çeşitli kriyoprotektan ajanların (KPA'lar) farklı konsantrasyonları ve kombinasyonları test edilmiştir. Ayrıca, dondurarak kurutma (D-kurutma) ve sıvı kurutma (S-kurutma) tekniklerinin optimizasyonu gerçekleştirilmiştir. Liyofilize edilmiş ürünler, 1, 15 ve 30 hafta sonra TA'yı parçalama yeteneği ve mikroorganizma canlılığı açısından değerlendirilmiştir. Sonuçlar, 30 hafta sonunda en yüksek canlılığın skim milk ile muamele edilen örneklerde gözlemlendiğini ortaya koymaktadır. Ayrıca, D-kurutma yöntemi, tüm kriyoprotektan türleri için S-kurutma yöntemine kıyasla üstün performans sergilemiştir.

Anahtar Kelimeler

Biyodegradasyon , Arıtım bakterileri , Kriyoprotektanlar , Liyofilizasyon , Terftalik asit

Lyophilization and Preservation Techniques for Extended Storage of Terephthalic acid Degrading Pseudomonas sp: Procedural Steps and Protective Agents

Öz

The uncontrolled release of industrial waste into nature leads to increased environmental pollution, posing serious threats to ecosystem balance. Terephthalic acid (TA) is a fundamental component used in the production of polyethylene terephthalate (PET), which finds applications in a wide range of industrial products, including plastic bottles, polyester fibers, and packaging materials. Effectively removing TA from wastewater systems can be achieved through various biotechnological methods. In this context, bioremediation bacteria play a critical role in environmental protection by providing sustainable solutions for wastewater management. The aim of this study is to investigate the effects of different protective and lyophilization methods on the biodegradation activity and bacterial viability of Pseudomonas sp. over specific storage periods. Various cryoprotectant agents (CPAs), including skim milk, sucrose, silica gel, and nutrient broth, were tested in different concentrations and combinations to improve storage conditions. Furthermore, optimization of freeze-drying (FD) and liquid drying (SD) techniques was conducted. The lyophilized products were evaluated for their ability to degrade TA and for microorganism viability after 1, 15, and 30 weeks. Results indicated that the highest viability after 30 weeks was observed in samples treated with skim milk. Additionally, the freeze-drying method demonstrated superior performance compared to liquid-drying method for all cryoprotectant types.

Anahtar Kelimeler

Biodegradation , Freeze-drying , Liquid-drying , Lyophilization , Protective agent , Terephthalic acid

Kaynakça

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