Torpedo EC Pestisitinin Ames Salmonella/Mikrozom Metodu ile Mutajenitesinin Değerlendirilmesi

Yıl 2025, Cilt: 25 Sayı: 5, 984 - 989, 01.10.2025

Arzu Özkara

https://doi.org/10.35414/akufemubid.1613988

Öz

Bu çalışmada avermectin grubu bir pestisit olan abamektinin ticari formu Torpedo EC’nin Ames/Salmonella mikrozom testi ile mutajenitesinin değerlendirilmesi gerçekleştirilmiştir. Test Salmonella typhimurium’a ait TA98 ve TA100 suşlarında S9 fraksiyonu varlığında ve yokluğunda iki aşamalı olarak yapılmıştır. Uygulanacak dozların belirlenmesi için test maddesinin sitotoksik dozları belirlenmiş ve deney için sitotoksik olmayan 6 farklı test dozu (1000, 800, 400, 200, 100, 50 μg/plak) üç tekrarlı olarak çalışılmıştır. Elde edilen veriler Dunnett-t testi kullanılarak istatistiksel açıdan değerlendirmeye alınmış ve negatif kontrol grubuyla karşılaştırılarak mutajenik aktivite değerlendirilmesi yapılmıştır. Çalışma sonucuna göre, Torpedo EC’nin 1000 μg/plak dozu hem S9 fraksiyonu varlığı hem de yokluğunda TA98 ve TA100 suşları üzerinde muatjenik aktivite gösterdiği tespit edilmiştir.

Anahtar Kelimeler

Abamektin , Akarisit , Ames Test , Mutajenite , Torpedeo EC

Evaluation of Mutagenicity of Torpedo EC Pesticide by Ames Salmonella/Microsome Method

Öz

In this study, the mutagenicity of Torpedo EC, a commercial form of abamectin, a pesticide from the Avermectin group, was evaluated using the Ames/Salmonella microsome test. The test was performed in two stages on TA98 and TA100 strains of Salmonella typhimurium in the presence and absence of S9 fraction. In order to determine the doses to be applied, cytotoxic doses of the test substance were determined and 6 different non-cytotoxic test doses (1000, 800, 400, 200, 100, 50 μg/plate) were studied in triplicate. The obtained data were statistically evaluated using the Dunnett-t test and mutagenic activity was evaluated by comparing with the negative control group. According to the study results, it was determined that Torpedo EC at a dose of 1000 μg/plate showed mutagenic activity on TA98 and TA100 strains both in the presence and absence of S9 fraction.

Anahtar Kelimeler

Abamectin , Acaricide , Ames Test , mutagenicity , Torpedo EC

Kaynakça

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