Saccharomyces Cerevisiae Sulu Lizatı ile Sentezlenen Çinko Oksit Nanopartiküllerinin In-Vitro Yara İyileşmesi Modelinde Etkilerinin İncelenmesi

Öz

Amaç: Bu çalışmanın amacı hamur mayası (Saccharomyces cerevisiae) sulu lizatı ile sentezlenen çinko oksit nanopartiküllerinin (ZnONPs) L929 fare fibroblast hücreleri üzerindeki toksik ve yara iyileştirici etkilerini incelemektir.
Yöntem: Saccharomyces cerevisiae sulu lizatı kullanılarak çinko oksit nanopartikülleri mikrodalga yöntemiyle sentezlenmiştir. ZnONPs karakterizasyonu Ultraviyole-Görünür bölge spektroskopisi (UV-Vis), SEM ve Zeta sizer ile gerçekleştirilmiştir. 1, 10, 100, 1000 µg/mL konsantrasyondaki ZnONPs’lerin toksik davranışları ve yara iyileşmesi üzerindeki etkileri in-vitro olarak L929 hücrelerinde incelenmiştir.
Bulgular: UV spektrumunda ZnONPs’ye spesifik 360-380 nm’de keskin pik görülmüştür. Zeta analizinde ZnO nanopartiküllerinin ortalama boyutu 512.8±16 nm ve zeta yükü ise -30.38±3.12 mV olarak ölçülmüştür. ZnONPs uygulanan L929 hücrelerinin doza bağımlı olarak toksik etki göstermediği bulunmuştur. 10, 100 ve 1000 µg/mL ZnONPs uygulanan L929 hücrelerinin yara kapanması miktarında kontrol grubu hücrelerine göre anlamlı oranda artış tespit edilmiştir.
Sonuç: Saccharomyces cerevisiae sulu lizatı ile sentezlenen çinko oksit (ZnO) nanopartiküllerinin in-vitro yara iyileştirici etkileri bu nanopartiküllerin ilaç ve kozmetik endüstrisinde kullanılabilme potansiyeli olduğunu göstermektedir.

Anahtar Kelimeler

• Çinko oksit
• Nanopartikül
• Yara iyileşmesi
• Saccharomyces cerevisiae
• Hücre proliferasyonu
• Cell proliferation

Investigation of The Effects of Zinc Oxide Nanoparticles Synthesized By Saccharomyces Cerevisiae Aqueous Lysate on In-Vitro Wound Healing Model

Öz

Objective: The objective of this study was to examine the toxic and wound-healing behaviours of zinc oxide (ZnO) nanoparticles synthesized with an aqueous lysate of sourdough (Saccharomyces cerevisiae) on L929 mouse fibroblast cells.
Method: Zinc oxide nanoparticles were synthesized by microwave method using the aqueous lysate of Saccharomyces cerevisiae. Characterization of ZnO nanoparticles was accomplished with Ultraviolet-Visible region spectroscopy (UV-Vis), SEM and Zeta sizer. The toxic behavior of ZnONPs at concentrations of 1, 10, 100, 1000 µg/mL and their effects on wound healing were investigated in-vitro in L929 cells.
Results: A sharp peak was observed at 360-380 nm specific to ZnO in the UV spectrum. In the zeta analysis, the mean size of ZnO nanoparticles was 512.8±16 nm and the zeta charge was -30.38±3.12 mV. It was found that L929 cells treated with ZnONPs did not show dose-dependent manner. A significant increase was found in the wound closure amount of L929 cells applied 10, 100 and 1000 µg/mL ZnONPs compared to the control group cells.
Conclusion: In-vitro wound healing effects of zinc oxide (ZnO) nanoparticles synthesized with Saccharomyces cerevisiae aqueous lysate show that these nanoparticles have the potential to be used in the pharmaceutical and cosmetic industries.

Anahtar Kelimeler

• Zinc Oxide
• Nanoparticles
• Wound Healing
• Saccharomyces cerevisiae
• Cell proliferation

Kaynakça

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