Egzersize Bağlı Kilo Kaybı ve Glutatyon S-Transferaz

Yıl 2021, Cilt: 5 Sayı: 3, 575 - 582, 30.09.2021

Adem Keskin Aslıhan Büyüköztürk Karul

https://doi.org/10.46237/amusbfd.941286

Öz

Amaç: Bu çalışmada, egzersiz yaptırılan ratlarda gözlenen kilo kaybının, Glutatyon S-Transferaz enzim aktivitesi üzerine etkisinin araştırılması amaçlanmıştır.
Yöntem: Ratlara 10 günlük egzersiz yaptırıldı. Gruplar arası kilo kaybı farkı oluşması amacıyla bir gruba melatonin ve niasin verildi. Çalışma boyunca ratlar tartıldı. Son egzersizden bir gün sonra intrakardiyak kan örnekleri alındı. Glutatyon S-Transferaz enzim aktivitesi ölçüldü.
Bulgular: Gruplarda kilo kaybı gözlendi. Vücut ağırlığı dikkate alındığında, melatonin ve niasin verilen grupta oluşan kilo kaybının anlamlı olduğu gözlendi (p<0.001). Kilo kaybı daha yüksek olan bu grubun Glutatyon S-Transferaz enzim aktivitesi, diğer gruptan daha düşük olduğu gözlendi (p<0.001). Gruplarda gözlenen kilo kaybı ile analizi yapılan Glutatyon S-Transferaz enzim aktivite düzeyleri arasında zıt yönlü korelasyon olduğu gözlendi (p<0.001).
Sonuç: Bir grupta yaklaşık %4 düzeyinde kilo kaybı oluştu. Egzersize bağlı kilo kaybı ile Glutatyon S-Transferaz enzim aktivitesi arasında zıt yönde bir korelasyon olduğu saptandı.  

Anahtar Kelimeler

Egzersiz, Kilo Kaybı, Glutatyon S-Transferaz

Destekleyen Kurum

Aydın Adnan Menderes Üniversitesi Bilimsel Araştırma Projeleri Birimi

Proje Numarası

TPF 19011

Teşekkür

Bu çalışma, Aydın Adnan Menderes Üniversitesi Bilimsel Araştırma Projeleri Birimi tarafından TPF 19011 proje numarası ile desteklenmiştir. Çalışma sürecinde yardımlarından dolayı ilgili birime şükranlarımızı sunarız.

Exercise-induced Weight Loss and Glutathione S-Transferase

Öz

Objective: In this study, it was aimed to investigate the effect of weight loss observed in exercised rats on Glutathione S-Transferase enzyme activity.
Methods: The rats were exercised for 10 days. Melatonin and niacin were given to one group in order to create a difference in weight loss between groups. The rats were weighed throughout the study. Intracardiac blood samples were taken, one day after the last exercise. Glutathione S-Transferase enzyme activity was measured.
Results: Weight loss was observed in the groups. It was observed that the weight loss in the melatonin and niacin group was significant, considering body weight (p<0.001). Glutathione S-Transferase enzyme activity of this group with higher weight loss was observed to be lower than the other group (p<0.001). There was an inverse correlation between the weight loss observed in the groups and the analyzed Glutathione S-Transferase enzyme activity levels (p<0.001).
Conclusion: Approximately 4% weight loss occurred in one group. There was an inverse correlation between exercise-induced weight loss and Glutathione S-Transferase enzyme activity.

Anahtar Kelimeler

Exercise, Weight Loss, Glutathione S-Transferase

Proje Numarası

TPF 19011

Kaynakça

• 1. Ward, Z. J., Long, M. W., Resch, S. C., Gortmaker, S. L., Cradock, A. L., Giles, C., et al. (2016). Redrawing The US Obesity Landscape: Bias-Corrected Estimates Of State-Specific Adult Obesity Prevalence. PLoS ONE, 11(3), e0150735.
• 2. Jensen, M. D, Ryan, D. H, Apovian, C. M, Ard, J. D., Comuzzie A. G., Donato K. A., et al. (2014). 2013 AHA/ACC/ TOS Guideline for The Management of Overweight and Obesity in Adults: A Report of The American College of Cardiology/American Heart Association Task Force On Practice Guidelines and The Obesity Society. Circulation, 129(2), 102–138.
• 3. Chin, S. H., Kahathuduwa, C. N., Binks M. (2016). Physical activity and obesity: what we know and what we need to know. Obesity Reviews, 17(12), 1226-1244.
• 4. Catenacci, V. A., Grunwald, G. K., Ingebrigtsen, J. P., Jakicic, J. M., McDermott, M. D., Phelan, S., et al. (2011). Physical Activity Patterns Using Accelerometry in the National Weight Control Registry. Obesity (Silver Spring), 19(6), 1163–1170.
• 5. Donnelly, J., Smith, B., Jacobsen, D., Kirk, E., DuBose K., Hyder M., et al. (2004). The role of exercise for weight loss and maintenance. Best Practice & Research Clinical Gastroenterology, 18(6), 1009-1029.
• 6. MacLean, P. S., Higgins, J. A., Wyatt, H. R., Edward L. (2009). Regular exercise attenuates the metabolic drive to regain weight after long-term weight loss. American Journal of Physiology- Regulatory, Integrative and Comparative Physiology, 297(3), 793-802.
• 7. Doucet, E., King, N., Levine, J. A., Ross, R. (2011). Update on exercise and weight control. Journal of Obesity, Article ID 358205, 3 pages.
• 8. Child, R. B., Wilkinson, D. M., Fallowfield, J. L., Donnelly, A. E. (1998). Elevated serum antioxidant capacity and plasma malondialdehyde concentration in response to a simulated half- marathon run. Medicine & Science Sports &Exercise, 30, 1603–1607.
• 9. Ashtary-Larky, D., Ghanavati, M., Lamuchi, N., Payami, S. A., Alavi-Rad, S., Boustaninejad, M., et al. (2017). Rapid weight loss vs. slow weight loss: which is more effective on body composition and metabolic risk factors?. International Journal of Endocrinology and Metabolism, 15(3), e13249.
• 10. Da Luz, F. Q., Hay, P., Gibson, A. A., Touyz, S. W., Swinbourne, J. M., Roekenes, J. A., et al. (2015). Does severe dietary energy restriction increase binge eating in overweight or obese individuals? A systematic review. Obesity Reviews, 16(8) 652–665.
• 11. Enayati, A. A., Ranson, H., Hemingway, J., (2005). Insect glutathione transferases and insecticide resistance. Insect Molecular Biology, 14, 3-8.
• 12. Hayes, J. D., Flanagan, J. U., Jowsey, I. R. (2005). Glutathione transferases. Annual Review of Pharmacology and Toxicology, 45, 51-88.
• 13. Jones, C. I., Zhu, H., Martin, S. F., Han, Z., Li, Y., Alevriadou, B. R. (2007). Regulation of antioxidants and phase 2 enzymes by shear-induced reactive oxygen species in endothelial cells. Annals of Biomedical Engineering, 35, 683–693.
• 14. Gronowska Senger, A., Gornicka, M., Kotodziejska, K. (2009). Tocopherol acetate vs. oxidative stress induced by physical exercise in rats. Polish Journal of Food and Nutrition Sciences, 59, 263-269.
• 15. Canto, C., Houtkooper, R. H., Pirinen, E., Youn, D. Y., Oosterveer, M. H., Cen, Y., et al. (2012). The NAD(+) precursor nicotinamide riboside enhances oxidative metabolism and protects against high-fat diet-induced obesity. Cell Metabolism, 15, 838–847.
• 16. Mostafavi, S. A., Akhondzadeh, S, Reza Mohammadi, M., Keshtkar, A. A., Hosseini, S., Reza Eshraghian, M., et al. (2017) Role of melatonin in body weight: A systematic review and meta- analysis. Current Pharmaceutical Design, 23(8), 3445-3452.
• 17. Reiter, R. J., Mayo, J. C., Tan, D. X., Sainz, R. M., Alatorre-Jimenez, M. Qin, L. (2016). Melatonin as an antioxidant: under promises but over delivers. Journal of Pineal Research, 61, 253-278.
• 18. Kwon, W. Y., Suh, G. J., Kim, K. S., Jung, Y. S., Kim, S. H., Lee, R., et al. (2018). Niacin and selenium attenuates brain ınjury after cardiac arrest by upregulatıng Dj-1-Akt Signaling. Critical Care Medicine, 46 (1), 125.
• 19. Aguilo, A., Tauler, P., Pilar, Guix, M., Villa, G., Cordova, A., Tur, J., et al. (2003). Effect of exercise intensity and training on antioxidants and cholesterol profile in cyclists. The Journal of Nutritional Biochemistry, 14, 319-325.
• 20. Radak, Z., Sasvari, M., Nyakas, C., Pucsok, J., Nakamoto, H., Goto, S. (2000). Exercise precondition in gagainst hydrogen peroxide induced oxidative damage in proteins of rat myocardium. Archives of Biochemistry and Biophysics, 376, 248-251.
• 21. Atalay, M., Oksala, N. K., Laaksonen, D. E., Savita, K., Chitose, N., Jani, L., et al. (2004). Exercise training modulates heat shock protein response in diabetic rats. Journal of Applied Physiology, 97, 605-611.
• 22. Banerjee, A. K., Mandal, A., Chanda, D., Chakraborti, S. (2003). Oxidant, antioxidant and physical exercise. Molecular and Cellular Biochemistry, 253, 307-312.
• 23. White, A., Estrada, M., Walker, K., Wisnia, P., Filgueira, G., Valdes, F., et al. (2001). Role of exercise and a scorbate on plasma antioxidant capacity in thorough bredracehorses. Comparative Biochemistry and Physiology, Part A Molecular and Integrative Physiology, 128, 99-104.
• 24. Çetin İ., Muhtaroğlu S., Keti D., Hatipoğlu N., Kurtoğlu S. (2013) Obez çocuklarda malondialdehit seviyesi ve paraoksonaz 1 aktivitesinin değerlendirilmesi. Sağlık Bilimleri Dergisi (Journal of Health Sciences) 22(1), 64-69.
• 25. Mohn, A., Catino, M., Capanna, R., Giannini, C., Marcovecchio, R., Chiarelli, F. (2005). Increased oxidative stress in prepubertal severely obese children: effect of a dietary restriction-weight loss program. The Journal of Clinical Endocrinology&Metabolism, 90(5), 2653-2658.
• 26. Sindhu, R. K., Koo, J. R., Roberts, C. K., Vaziri, N. D. (2004). Dysregulation of hepatic superoxide dismutase, catalase and glutathione peroxidase in diabetes: response to insulin and antioxidant therapies. Clinical and Experimental Hypertension, 26(1), 43-53.
• 27. Afshari, F., Ghosh, S., Khazaei, M., Kieffer, T. J., Brownsey, R. W., Laher, I. (2008). Exercise restores endothelial function independently of weight loss or hyperglycaemic status in db/dbmice. Diabetologia, 51, 1327–1337.
• 28. Flores, M. L., Nieto, N. L., Moreira, O. C., Iglesias, D. S. (2018). Effects of melatonin on sports performance: A systematic review. Journal of Exercise Physiology, 21(5), 121-138.
• 29. Stacchiotti, A., Favero, G., Rodella, L. F. (2020). Impact of melatonin on skeletal muscle and exercise. Cells, 9 (2), 288.