Comparative Biplot Analysis of Micropropagation of Viking Aronia Cultivar in Different Plant Tissue Culture Media
Öz
In this study, the effect of different concentrations of some plant growth regulators on the in vitro micropropagation of the Viking aronia variety was investigated. In this context, 15 different culture media were prepared by adding plant growth regulators (BAP, IBA, GA3, TDZ) in various concentrations and combinations to the MS medium (Murashige and Skoog, 1962). Sterilised shoot tips were transferred to the relevant plant tissue culture media in three replications according to the randomized parcels trial pattern, with three explants in each replication. At the end of the four-week development period, average vitrification rate (VIR), average shoot length (SHL) and average number of nodes (NON) parameters were evaluated. Biplot analysis was employed to analyze the data obtained. According to the biplot analysis, B1I002G1T1: (1.0 mg L-1 BAP+0.02 mg L-1 IBA+1.0 mg L-1 GA3+1.0 mg L-1 TDZ), B1I002G1T05: (1.0 mg L-1 BAP+0.02 mg L-1 IBA+1 mg L-1 GA3+0.5 mg L-1 TDZ) and B1I002G05T1: (1.0 mg L-1 BAP + 0.02 mg L-1 IBA+0.5 mg L-1 GA3+1.0 mg L-1 TDZ) had a more positive effect on the number of nodes and shoot length. This study showed that the Viking aronia variety can be easily grown from cuttings by adjusting the plant growth regulator concentrations and combinations. Different media and plant growth regulators are needed for each aronia variety, so more studies are needed to improve success rates.
Anahtar Kelimeler
Aronia melanocarpa (Michx.) Elliott, explant, plant growth regulators, plant tissue culture, micropropagation
Kaynakça
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