In Vitro Propagation of Medicinal and Ornamental Plant Oxalis triangularis A.st.-Hil.

Abstract

An in vitro propagation protocol of Oxalis triangularis A.st.-Hil, a valuable medicinal and ornamental plant, has been developed. In experimental studies, α-Naphthalene acetic acid (NAA) (0-0.5-1- 2 mg/L) and 6-Benzylaminopurine (BAP) (0.5-1 mg/L) plant growth regulators were applied together or alone to O. triangularis leaf and petiole explants. The best shoot formation per explant and the best leaf formation were obtained as 5.67 ± 2.43 and 4.00 ± 1.86 from leaf explants cultured in Murashige and Skoog (MS) medium containing 2 mg/L NAA and 0.5 mg/L BAP plant growth regulators, respectively. The shoots obtained were transferred to MS medium containing 0.5 mg/L NAA for root development. The plants obtained as a result of micropropagation processes were successfully acclimatized.

Keywords

• Plant tissue culture
• micropropagation
• auxin
• cytokinin
• Oxalis triangularis

Tıbbi ve Süs Bitkisi Oxalis triangularis A.st.-Hil. 'in In Vitro Çoğaltımı

Öz

Tıbbi ve süs bitkisi olarak değerli bir bitki olan Oxalis triangularis'in in vitro çoğaltım protokolü belirlenmiştir. Deneysel çalışmalarda Oxalis triangularis yaprak ve petiol eksplantlarına, α-Naftalin asetik asit (NAA) (0-0.5-1- 2 mg/L) ve 6-Benzilaminopurin (BAP) (0.5-1 mg/L) bitki büyüme düzenleyicileri birlikte veya tek başına uygulanmıştır. Eksplant başına en iyi sürgün oluşumu ve en iyi yaprak oluşumu, sırasıyla 2 mg/L NAA ve 0.5 mg/L BAP bitki büyüme düzenleyicilerini içeren Murashige ve Skoog (MS) besiyerinde kültüre alınan yaprak eksplantlarında 5.67 adet ± 2,43 ve 4.00 ± 1,86 olarak elde edilmiştir. Elde edilen sürgünler kök gelişimi için 0.5 mg/L NAA içeren MS besin ortamına aktarılmıştır. Mikroçoğaltım işlemleri sonucunda elde edilen bitkiler başarıyla aklimatize edilmiştir.

Anahtar Kelimeler

• Bitki doku kültürü
• mikroçoğaltım
• oksin
• sitokinin
• Oxalis triangularis

Kaynakça

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