Determination of Clone Lines Having a High Content of Stevioside and Rebaudioside A for In Vitro Commercial Production of Stevia rebaudiana

Öz

  Hem sağlık hem de gıda sektörü için önemli bir bitki olan Stevia rebaudiana Bertoni, kalorisi olmayan tatlandırıcı bileşikler içermektedir ve stevia-kaynaklı sentetik olmayan bu tatlandırıcılar için talepte sürekli bir artış söz konusudur. Mevcut çalışmanın amacı, S. rebaudiana’nın in vitro ticari üretimi için yüksek steviosid ve rebaudiosid A içeriğine sahip klonların belirlenmesidir. Bu amaçla, Stevia tohumları in vitro koşullarda kültüre alınmış ve yüksek çoğaltım oranı ve yüksek steviosid ve rebaudiosid A içeriği gösteren klon hatları seçilmiştir. Elde edilen iki klon hattı ticari bir üretici tarafından Stevia’nın ticari üretimi için adapte edilmiştir. 

 

Anahtar Kelimeler

• Stevia rebaudiana,in vitro seed germination,in vitro commercial production,HPLC,stevioside,rebaudioside A

Kaynakça

1. Ahmed, M. B., M. Salahin, R. Karim, M. A. Razvy, M. M. Hannan, R. Sultana, M. Hossain, and R. Islam. 2007. An efficient method for in vitro clonal propagation of a newly introduced sweetener plant (Stevia rebaudiana Bertoni) in Bangladesh. J. Sci. Res. 2: 121-125.
2. Ali, A., I. Gull, S. Naz, and S. Afghan. 2010. Biochemical investigation during different stages of in vitro propagation of Stevia rebaudiana. Pak. J. Bot. 42 (4): 2827-2837.
3. Bayraktar, M., S. Hayta, S., Parlak, and A. Gurel. 2015. Micropropagation of centennial tertiary relict trees of Liquidambar orientalis Miller through meristematic nodules produced by cultures of primordial shoots, Trees, 29 (4): 999-1009. doi: 10.1007/s00468-015-1179-2.
4. Bondarev, N., O. Reshetnyak, and A. Nosov. 2001. Peculiarities of diterpenoidsteviol glycoside production in in vitro cultures of Stevia rebaudiana Bertoni. Plant Sci. 161: 155-163.
5. Bondarev, N., O. Reshetnyak, and A. Nosov. 2003. Effects of nutrient medium composition on development of Stevia rebaudiana shoots cultivated in the roller bioreactor and their production of steviol glycosides. Plant Sci. 165: 845-850.
6. Brandle, J. E., A. N. Starrat, and M. Gijen. 1998. Stevia rebaudiana: Its agricultural, biological, and chemical properties. Can. J. of Plant Sci. 78: 527-536.
7. Brandle, J. E., and P. G. Telme. 2007. Steviol glycoside biosynthesis.Phtochemistry 68: 1855-1863.
8. Erkucuk, A., I. H. Akgun, and O. Y. Celiktas. 2009. Supercritical CO2 extraction of glycosides from Stevia rebaudiana leaves: Identification and optimization. J. Supercrit. Fluid. 51: 29-35.

9. Gamborg, O. L., R. A. Miller, and K. Ojima.1968. Nutrient requirements of suspension cultures of soybean root cells. Exp.Cell. Res. 50 (1): 151-158.
10. Giridhar, P., K. S. Sowmya, A. Ramakrishna, and G. A. Ravishankar. 2010. Rapid clonal propagation and stevioside profiles of Stevia rebaudiana Bertoni. Int. J. Plant Dev. Biol. 4 (1): 47-52.
11. Hwang, S. J. 2006. Rapid in vitro propagation and enhanced stevioside accumulation in Stevia rebaudiana Bert. J. Plant Biol. 49 (4): 267-270.
12. Ibrahim, I. A., M. I. Nasr, B. R. Mohammed, and M. M. El-Zefzafi. 2008. Plant growth regulators affecting in vitro cultivation of Stevia rebaudiana. Sugar Tech. 10: 254-259.
13. Khalil, S. A., R. Zamir, and N. Ahmad. 2014. Selection of suitable propagation method for consistent plantlets production in Stevia rebaudiana (Bertoni). Saud. J. Biol. Sci. 21 (6): 566-573.
14. Koyama, E., K. Kitazawa, Y. Ohori, O. Izawa, K. Kakegawa, A. Fujino, and M. Ui. 2003. In vitro metabolism of the glycosidics weeteners, stevia mixture and enzymatically modified stevia in human intestinal microflora. Food and Chemical Toxicology. 41: 359-374.
15. Ladygin, V. G., N. I. Bondarev, G. A. Semenova, A. A. Smolov, O. V. Reshetnyak, and A. M. Nosov. 2008. Chloroplast ultrastructure, photosynthetic apparatus activities and production of steviol glycosides in Stevia rebaudiana in vivo and in vitro. Biologia Plantarum 52: 9-16.
16. Lata, H., S. Chandra, Y. H. Wang, V. Raman, and I. A. Khan. 2013. TDZ-induced high frequency plant regeneration through direct shoot organogenesis in Stevia rebaudianaBertoni: an important medicinal plant and a natural sweetener. Am. J. Plant Sci. 4: 117-128.
17. Lemus-Mondaca, R., A. Vega-Gálvez, L. Zura-Bravo, and K. Ah-Hen. 2012. Stevia rebaudiana Bertoni, source of a high-potency natural sweetener: A comprehensive review on the biochemical, nutritional and functional aspects. Food Chem. 132: 1121-1132.
18. Lloyd, G., and B. McCown. 1980. Commercially-feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot tip culture. Int. Plant Prop. Soc. 30: 421-427.
19. Madan, S., S. Ahmad, G. N. Singh, K. Kohli, Y. Kumar, R. Singh, and M. Garg. 2010. Stevia rebaudiana (Bert.) Bertoni – A Review.Indian J. Nat. Prod. Res. 1 (3): 267-286.
20. Modi, A., N. Litoriya, V. Prajapati, R. Rafalia, and S. Narayanan. 2014. Transcriptional profiling of genes involved in steviol glycoside biosynthesis in Stevia rebaudiana Bertoni during plant hardening. Dev. Dyn. 243: 1067–1073.
21. Murashige, T., and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497.
22. Rajasekaran, T., P. Giridhar, and G. A. Ravishankar. 2007. Production of steviosides in ex vitro and in vitro grown Stevia rebaudiana Bertoni. J. Sci. Food Agric. 87: 420–424.
23. Reis, R. V., A.P.P.L. Borges, T. P. C. Chierrito, E. R. de Souto, L. M. de Souza, M. Iacomini, A. J. B. de Oliveira, and R.A.C. Gonçalves. 2011. Establishment of adventitious root culture of Stevia rebaudiana Bertoni in a roller bottle system. Plant Cell Tiss. Organ Cult. 106: 329–335.
24. Richman, A. S., M. Gijzen, A. N. Starratt, Z. Yang, and J. E. Brandle. 1999. Diterpene synthesis in Stevia rebaudiana: recruitment and up-regulation of key enzymes from the gibberellin biosynthetic pathway. Plant J. 19 (4): 411–421.
25. Sairkar, P., M. K. Chandravanshi, N. P. Shukla, and N. N. Mehrotra. 2009. Mass production of an economically important medicinal plant Stevia rebaudiana using in vitro propagation techniques. J. Med. Plants Res. 3 (4): 266–270.
26. Satpathy, S., and M. Da. 2010. In vitro shoot multiplication in Stevia rebaudiana Bert., a medicinally important plant. Gen. Appl. Plant Physiol. 36 (3-4): 167–175.
27. Singh, S. D., and G. P. Rao. 2005. Stevia: The Herbal Sugar of 21st Century. Sugar Tech. 7 (l): 17–24. Sivaram, L., and U. Mukundam. 2003. In vitro culture studies on Stevia rebaudiana. In vitro Cell. Dev.Biol. Plant 39: 520–523.
28. Soejarto, D. D., A. D. Kinghorn, and N. R. Farnsworth. 1982. Potential sweetening agents of plant origin. III. Organoleptic evaluation of stevia leaf herbarium samples for sweetness. J. Nat. Prod. 45: 590–599.
29. Soejarto, D. D., C. M. Compadre, P. J. Medon, S. K. Kamath, and A. D. Kinghorn. 1983. Potential sweetening agents of plant origin. II. Field search for sweet-tasting Stevia species. Econ. Bot. 37: 71–79.
30. Tadhani, M. B., V. H. Patel, and R. Subhash. 2007. In vitro antioxidant activities of Stevia rebaudiana leaves and callus. J. Food Comp. Analysis 20: 323–329.