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Determination of Clone Lines Having a High Content of Stevioside and Rebaudioside A for In Vitro Commercial Production of Stevia rebaudiana

Cilt: 25 Sayı: 1 30 Haziran 2015
Meltem Bayraktar , Bärbel Rock Okuyucu
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Determination of Clone Lines Having a High Content of Stevioside and Rebaudioside A for In Vitro Commercial Production of Stevia rebaudiana

Öz

  Hem sağlık hem de gıda sektörü için önemli bir bitki olan Stevia rebaudiana Bertoni, kalorisi olmayan tatlandırıcı bileşikler içermektedir ve stevia-kaynaklı sentetik olmayan bu tatlandırıcılar için talepte sürekli bir artış söz konusudur. Mevcut çalışmanın amacı, S. rebaudiana’nın in vitro ticari üretimi için yüksek steviosid ve rebaudiosid A içeriğine sahip klonların belirlenmesidir. Bu amaçla, Stevia tohumları in vitro koşullarda kültüre alınmış ve yüksek çoğaltım oranı ve yüksek steviosid ve rebaudiosid A içeriği gösteren klon hatları seçilmiştir. Elde edilen iki klon hattı ticari bir üretici tarafından Stevia’nın ticari üretimi için adapte edilmiştir. 

 


Anahtar Kelimeler

Stevia rebaudiana,in vitro seed germination,in vitro commercial production,HPLC,stevioside,rebaudioside A

Kaynakça

  1. Ahmed, M. B., M. Salahin, R. Karim, M. A. Razvy, M. M. Hannan, R. Sultana, M. Hossain, and R. Islam. 2007. An efficient method for in vitro clonal propagation of a newly introduced sweetener plant (Stevia rebaudiana Bertoni) in Bangladesh. J. Sci. Res. 2: 121-125.
  2. Ali, A., I. Gull, S. Naz, and S. Afghan. 2010. Biochemical investigation during different stages of in vitro propagation of Stevia rebaudiana. Pak. J. Bot. 42 (4): 2827-2837.
  3. Bayraktar, M., S. Hayta, S., Parlak, and A. Gurel. 2015. Micropropagation of centennial tertiary relict trees of Liquidambar orientalis Miller through meristematic nodules produced by cultures of primordial shoots, Trees, 29 (4): 999-1009. doi: 10.1007/s00468-015-1179-2.
  4. Bondarev, N., O. Reshetnyak, and A. Nosov. 2001. Peculiarities of diterpenoidsteviol glycoside production in in vitro cultures of Stevia rebaudiana Bertoni. Plant Sci. 161: 155-163.
  5. Bondarev, N., O. Reshetnyak, and A. Nosov. 2003. Effects of nutrient medium composition on development of Stevia rebaudiana shoots cultivated in the roller bioreactor and their production of steviol glycosides. Plant Sci. 165: 845-850.
  6. Brandle, J. E., A. N. Starrat, and M. Gijen. 1998. Stevia rebaudiana: Its agricultural, biological, and chemical properties. Can. J. of Plant Sci. 78: 527-536.
  7. Brandle, J. E., and P. G. Telme. 2007. Steviol glycoside biosynthesis.Phtochemistry 68: 1855-1863.
  8. Erkucuk, A., I. H. Akgun, and O. Y. Celiktas. 2009. Supercritical CO2 extraction of glycosides from Stevia rebaudiana leaves: Identification and optimization. J. Supercrit. Fluid. 51: 29-35.
  9. Gamborg, O. L., R. A. Miller, and K. Ojima.1968. Nutrient requirements of suspension cultures of soybean root cells. Exp.Cell. Res. 50 (1): 151-158.
  10. Giridhar, P., K. S. Sowmya, A. Ramakrishna, and G. A. Ravishankar. 2010. Rapid clonal propagation and stevioside profiles of Stevia rebaudiana Bertoni. Int. J. Plant Dev. Biol. 4 (1): 47-52.

Kaynak Göster

APA
Bayraktar, M., & Okuyucu, B. R. (2015). Determination of Clone Lines Having a High Content of Stevioside and Rebaudioside A for In Vitro Commercial Production of Stevia rebaudiana. ANADOLU Journal of Aegean Agricultural Research Institute, 25(1), 17-30. https://izlik.org/JA29BY48XR
AMA
1.Bayraktar M, Okuyucu BR. Determination of Clone Lines Having a High Content of Stevioside and Rebaudioside A for In Vitro Commercial Production of Stevia rebaudiana. ANADOLU. 2015;25(1):17-30. https://izlik.org/JA29BY48XR
Chicago
Bayraktar, Meltem, ve Bärbel Rock Okuyucu. 2015. “Determination of Clone Lines Having a High Content of Stevioside and Rebaudioside A for In Vitro Commercial Production of Stevia rebaudiana”. ANADOLU Journal of Aegean Agricultural Research Institute 25 (1): 17-30. https://izlik.org/JA29BY48XR.
EndNote
Bayraktar M, Okuyucu BR (01 Haziran 2015) Determination of Clone Lines Having a High Content of Stevioside and Rebaudioside A for In Vitro Commercial Production of Stevia rebaudiana. ANADOLU Journal of Aegean Agricultural Research Institute 25 1 17–30.
IEEE
[1]M. Bayraktar ve B. R. Okuyucu, “Determination of Clone Lines Having a High Content of Stevioside and Rebaudioside A for In Vitro Commercial Production of Stevia rebaudiana”, ANADOLU, c. 25, sy 1, ss. 17–30, Haz. 2015, [çevrimiçi]. Erişim adresi: https://izlik.org/JA29BY48XR
ISNAD
Bayraktar, Meltem - Okuyucu, Bärbel Rock. “Determination of Clone Lines Having a High Content of Stevioside and Rebaudioside A for In Vitro Commercial Production of Stevia rebaudiana”. ANADOLU Journal of Aegean Agricultural Research Institute 25/1 (01 Haziran 2015): 17-30. https://izlik.org/JA29BY48XR.
JAMA
1.Bayraktar M, Okuyucu BR. Determination of Clone Lines Having a High Content of Stevioside and Rebaudioside A for In Vitro Commercial Production of Stevia rebaudiana. ANADOLU. 2015;25:17–30.
MLA
Bayraktar, Meltem, ve Bärbel Rock Okuyucu. “Determination of Clone Lines Having a High Content of Stevioside and Rebaudioside A for In Vitro Commercial Production of Stevia rebaudiana”. ANADOLU Journal of Aegean Agricultural Research Institute, c. 25, sy 1, Haziran 2015, ss. 17-30, https://izlik.org/JA29BY48XR.
Vancouver
1.Meltem Bayraktar, Bärbel Rock Okuyucu. Determination of Clone Lines Having a High Content of Stevioside and Rebaudioside A for In Vitro Commercial Production of Stevia rebaudiana. ANADOLU [Internet]. 01 Haziran 2015;25(1):17-30. Erişim adresi: https://izlik.org/JA29BY48XR