Deneysel overyan iskemi/reperfüzyon modelinde iloprostun uzak organ olarak karaciğer dokusu üzerindeki koruyucu etkisi

Yıl 2022, , 71 - 76, 29.01.2022

Mehmet Duran , Nezir Yılmaz , Ebru Annaç , Mevlüt Doğukan

https://doi.org/10.21673/anadoluklin.1030797

Öz

Amaç: Bu deneysel çalışmada iloprostun karaciğer dokusu üzerindeki koruyucu etkisini bir iskemi/reperfüzyon (İ/R) sıçan modelinde araştırmak amaçlanmıştır.

Yöntem: Toplam 32 adet dişi Sprague–Dawley sıçan, 4 eşit gruba ayrıldı. Grup 1’deki sıçanlarda overyan dokulara herhangi bir cerrahi işlem uygulanmadı. Sırasıyla Grup 2, 3 ve 4’teki overyan dokulara ise 3 saatlik torsiyon, 3 saatlik torsiyon + 3 saatlik detorsiyon, ve 3 saatlik torsiyon + 3 saatlik detorsiyon + 60 dakikalık iloprost infüzyonu uygulandı. Dokulardan 7 μm kalınlıkta kesitler alındı, hematoksilen–eozin, Masson trikrom ve toluidin mavisi ile boyandı. Kesitler üzerinde ışık mikroskobik inceleme yapıldı.

Bulgular: Grup 2, 3 ve 4’te Grup 1’e kıyasla sinüzoidal dilatasyon ve vasküler konjesyonda istatistiksel olarak anlamlı artış tespit edildi (p<0,05). Grup 4’te diğer gruplara kıyasla daha fazla sinüzoidal dilatasyon ve vasküler konjesyon görüldü. Yine Grup 2, 3 ve 4’te Grup 1’e kıyasla daha fazla enflamasyon, fibrozis, hemoraji ve daha yüksek mast hücre yoğunluğu tespit edildi. Ancak fark istatistiksel olarak anlamlı değildi (p>0,05). Histopatolojik skorlamada Grup 2 ve 4’te benzer, Grup 3’te ise daha yüksek skorlar gözlendi.

Sonuç: İloprostun İ/R hasarına karşı karaciğer dokusu üzerinde koruyucu etkileri olduğu sonucuna varılmıştır.

Anahtar Kelimeler

iloprost, iskemi/reperfüzyon, karaciğer hasarı

Destekleyen Kurum

yok

Proje Numarası

yok

Teşekkür

Doç.Dr. Öznur Uludağ

The protective effect of iloprost on liver tissue as a remote organ in an experimental model of ovarian ischemia/reperfusion

Öz

Aim: In this experimental study, we aimed to investigate the protective effect of iloprost on liver tissue in a rat model of ischemia/reperfusion (I/R).

Methods: A total of 32 female Sprague–Dawley rats were divided into 4 equal groups. The ovarian tissues of the rats in Group 1 did not undergo any surgical procedure. The ovarian tissues of Group 2, 3, and 4 underwent 3-hour of torsion, 3-hour detorsion + 3-hour torsion, and 3-hour torsion + 3-hour detorsion + 60-minute iloprost infusion, respectively. Sections of 7 μm thickness were taken, stained with hematoxylin–eosin, Masson trichrome, and toluidine blue, and examined with light microscopy.

Results: Compared to Group 1, a statistically significant increase in sinusoidal dilatation and vascular congestion was found in Group 2, 3, and 4 (p<0.05). Compared to the other groups, more sinusoidal dilatation and vascular congestion were observed in Group 4. Similarly, Group 2, 3, and 4 showed more inflammation, fibrosis and hemorrhage and higher mast cell density compared to Group 1, although the difference was not statistically significant (p>0.05). In the histopathological scoring, Group 2 and 4 had similar scores while Group 3 scored higher.

Conclusion: It was concluded that iloprost had protective effects on liver tissue against I/R injury.

Anahtar Kelimeler

iloprost, ischemia/reperfusion, liver injury

Proje Numarası

yok

Kaynakça

• Huchon C, Fauconnier A. Adnexal torsion: a literature review. Eur J Obstet Gynecol Reprod Biol. 2010;150(1):8–12.
• Sasaki KJ, Miller CE. Adnexal torsion: review of the literature. J Minim Invasive Gynecol. 2014;21(2):196–202.
• Harkins G. Ovarian torsion treated with untwisting: second look 36 hours after untwisting. J Minim Invasive Gynecol. 2007;14(3):270.
• Mazouni C, Bretelle F, Ménard JP, Blanc B, Gamerre M. Diagnosis of adnexal torsion and predictive factors of adnexal necrosis. Gynecol Obstet Fertil. 2005;33(3):102–6.
• Houry D, Abbott JT. Ovarian torsion: a fifteen-year review. Ann Emerg Med. 2001;38(2):156–9.
• Taskin O, Birincioglu M, Aydin A, Buhur A, Burak F, Yilmaz I, ve ark. The effects of twisted ischaemic adnexa managed by detorsion on ovarian viability and histology: an ischaemia–reperfusion rodent model. Hum Reprod. 1998;13:2823–7.
• Guile SL, Mathai JK. Ovarian torsion. Treasure Island, FL/ABD: StatPearls Publishing; 2021.
• Serracino-Inglott F, Habib NA, Mathie RT. Hepatic ischemia–reperfusion injury. Am J Surg. 2001;181(2):160–6.
• Zimmerman BJ, Granger DN. Reperfusion injury. Surg Clin North Am. 1992;72(1):65–83.
• Suleyman B, Albayrak A, Kurt N, Demirci E, Gundogdu C, Aksoy M. The effect of etoricoxib on kidney ischemia–reperfusion injury in rats: a biochemical and immunohistochemical assessment. Int Immunopharmacol. 2014;23:179–85.
• Yapca OE, Borekci B, Suleyman H. Ischemia–reperfusion damage. Eurasian J Med. 2013;45(2):126–7.
• el-Marasy SA, Abdel-Rahman RF, Abd-Elsalam RM. Neuroprotective effect of vildagliptin against cerebral ischemia in rats. Naunyn Schmiedebergs Arch Pharmacol. 2018;391(10):1133–45.
• Ergun Y, Koc A, Dolapcioglu K, Akaydin Y, Dogruer G, Kontas T, ve ark. The protective effect of erythropoietin and dimethylsulfoxide on ischemia–reperfusion injury in rat ovary. Eur J Obstet Gynecol Reprod Biol. 2010;152(2):186–90.
• Erer D, Dursun AD, Oktar GL, Iriz E, Zor MH, Elmas C, ve ark. The effects of iloprost on lung injury induced by skeletal muscle ischemia–reperfusion. Bratisl Lek Listy. 2014;115(7):405–10.
• Abramov Y, Golden B, Sullivan M, Botros SM, Miller JJR, Alshahrour A, ve ark. Histologic characterization of vaginal vs. abdominal surgical wound healing in a rabbit model. Wound Repair Regen. 2007;15(1):80–6.
• Peralta C, Jiménez-Castro MB, Gracia-Sancho J. Hepatic ischemia and reperfusion injury: effects on the liver sinusoidal milieu. J Hepatol. 2013;59(5):1094–106.
• Zhai Y, Petrowsky H, Hong JC, Busuttil RW, Kupiec-Weglinski JW. Ischaemia–reperfusion injury in liver transplantation—from bench to bedside. Nat Rev Gastroenterol Hepatol. 2013;10(2):79–89.
• Eltzschig HK, Collard CD. Vascular ischaemia and reperfusion injury. Br Med Bull. 2004;70:71–86.
• Videla LA. Oxidative stress signaling underlying liver disease and hepatoprotective mechanisms. World J Hepatol. 2009;1(1):72–8.
• Guo WA. The search for a magic bullet to fight multiple organ failure secondary to ischemia/reperfusion injury and abdominal compartment syndrome. J Surg Res. 2013;184(2):792–3.
• Sookoian S, Pirola CJ. Liver enzymes, metabolomics and genome-wide association studies: from systems biology to the personalized medicine. World J Gastroenterol. 2015;21(3):711–25.
• Honda M, Takeichi T, Asonuma K, Tanaka K, Kusunoki M, Inomata Y. Intravital imaging of neutrophil recruitment in hepatic ischemia–reperfusion injury in mice. Transplantation. 2013;95(4):551–8.
• Vedder NB, Fouty BW, Winn RK, Harlan JM, Rice CL. Role of neutrophils in generalized reperfusion injury associated with resuscitation from shock. Surgery. 1989;106(3):509–16.
• Montalvo-Jave EE, Escalante-Tattersfield T, Ortega-Salgado JA, Piña E, Geller DA. Factors in the pathophysiology of the liver ischemia–reperfusion injury. J Surg Res. 2008;147(1):153–9.
• Stewart RK, Dangi A, Huang C, Murase N, Kimura S, Stolz DB, ve ark. A novel mouse model of depletion of stellate cells clarifies their role in ischemia/reperfusion- and endotoxin-induced acute liver injury. J Hepatol. 2014;60(2):298–305.
• Mubarak KK. A review of prostaglandin analogs in the management of patients with pulmonary arterial hypertension. Respir Med. 2010;104(1):9–21.
• Baltalarli A, Ozcan V, Bir F, Aybek H, Sacar M, Onem G, ve ark. Ascorbic acid (vitamin C) and iloprost attenuate the lung injury caused by ischemia/reperfusion of the lower extremities of rats. Ann Vasc Surg. 2006;20(1):49–55.
• Emrecan B, Tulukoglu E, Bozok S, Kestelli M, Onem G, Küpelioglu A, ve ark. Effects of Iloprost and pentoxifylline on renal ischemia–reperfusion in rabbit model. Eur J Med Res. 2006;11(7):295–9.
• Caliskan A, Yavuz C, Karahan O, Yazici S, Guclu O, Demirtas S, ve ark. Iloprost reduces myocardial edema in a rat model of myocardial ischemia reperfusion. Perfusion. 2014;29(3):260–4.
• Karaçor T, Dogan Z, Elibol E, Bulbul M, Nacar MC. Effects of iloprost on experimental ischemia and reperfusion injury in rat ovary. Biotech Histochem. 2020;95(5):373–80.
• Çevirme D, Adademir T, Kafalı Başaran E, Şavluk ÖF, Elibol A, Erkanlı Şentürk G, ve ark. Comparison between iloprost and alprostadil for protection against ischemia/reperfusion injury in a rat model. Turk J Med Sci. 2018;48(3):661–9.
• Gedik E, Girgin S, Obay BD, Ozturk H, Ozturk H, Buyukbayram H. Iloprost, a prostacyclin (PGI2) analogue, reduces liver injury in hepatic ischemia–reperfusion in rats. Acta Cir Bras. 2009;24(3):226–32.