Antimicrobial Properties and Chemical Composition of the Essential Oil of Leucobryum glaucum (Leucobryaceae)

Yıl 2020, Cilt: 6 Sayı: 2, 112 - 118, 15.11.2020

Gonca Çelik

https://doi.org/10.26672/anatolianbryology.730445

Öz

In this study, a detailed study of the essential oil from Leucobryum glaucum (Hedw.) Angstr. was evaluated by gas chromatography/flame ionization detector (GC/FID) and gas chromatography/mass spectrometry (GC/MS) methods as well as screened for antibacterial activities of the essential oil and solvent extracts (n-hexane and methanol). According to the results, a total of 47 compounds were detected, among which thujopsadiene (35.5%) and β-curcumene (25.4%) were the main components. In the second part of this study, the essential oil and solvent extracts were tested for its antimicrobial activity against 9 microorganisms with minimal-inhibitory-concentration (MIC) values in the range 61-4235 µg / mL.

Anahtar Kelimeler

Leucobryum glaucum, Essential oil, Solvent extract, GC-FID/MS, Antimicrobial activity

Leucobryum glaucum (Leucobryaceae)'un Uçucu Yağının Kimyasal Bileşimi ve Antimikrobiyal Özellikleri

Öz

Bu çalışmada, Leucobryum glaucum (Hedw.) Angstr.'nin uçucu yağının detaylı çalışması gaz kromatografisi/ alev iyonlaştırma dedektörü (GC/FID) ve gaz kromatografisi/ kütle spektrometresi ile değerlendirildi hem de uçucu yağ ve çözücü ekstraktlarının (n-hekzan ve metanol) antimikrobiyal aktivitesi incelendi. Sonuçlara göre, thujopsadien (% 35.5) ve β-curcumen (% 25.4) başlıca bileşenler olmak üzere toplamda 47 bileşik belirlendi. Çalışmanın ikinci kısmında ise uçucu yağ ve çözücü ekstraktlarının antimiktobiyal aktivitesi 9 mikroorganizmaya karşı minimum-inhibisyon-konsantrasyonu (MİK) değerleri 61-4235 µg / mL aralığında test edildi.

Anahtar Kelimeler

Leucobryum glaucum, Uçucu yağ, Çözücü ekstraktı, GC-FID/MS, Antimikrobiyal aktivite

Kaynakça

• Adams R.P. 2004. Identification of essential oil components by gas chromatography/quadrupole mass spectroscopy. Carol Stream, IL: Allured Publication.
• Ahmad I. Mehmood Z. Mohammad F. 1998. Screening of some Indian medicinal plants for their antimicrobial properties. Journal of Ethnopharmacology. 62: 183-193.
• Barry A.L. Craig W.A. Nadler H. Reller L.B. Sander C.C. Swenson J.M. 1999. Methods for Determining Bactericidal Activity of antimicrobial Agents. Approved Guideline, NCCLS. 19: 1-29.
• Bicchi C. Cordero C. Liberto E. Sgorbini B. Rubiolo P. 2008. Headspace sampling of the volatile fraction of vegetable matrices. Journal of Chromatography A. 1184: 220-233.
• Boyom F.F. Ngouana V. Amvam Zollo P.H. Menut C. Bessiere J.M. Gut J. Rosenthal P.J. 2003. Composition and anti-plasmodial activities of essential oils from some Cameroonian medicinal plants. Phytochem. 64: 1269-1275.
• Cansu T.B. Yaylı B. Özdemir T. Batan N. Alpay Karaoğlu Ş. Yaylı N. 2013. Antimicrobial activity and chemical composition of mosses (Hylocomium splendens (Hedw.) Schimp. and Leucodon sciuroides (Hedw.) Schwägr) growing in Turkey. Turkish Journal of Chemistry. 37: 213-219.
• Fedosov V.E. Ignatova E.A. 2009. Tortella Densa (Pottiaceae, Bryophyta) in Russia. Arctoa. 18: 189-194.
• Jockovic N. Andrade P.B. Valentão P. Sabovljevic M. 2008. HPLC-DAD of phenolics in bryophytes Lunularia cruciata, Brachytheciastrum velutinum and Kindbergia praelonga. Journal of the Serbian Chamical Society. 73: 1161-1167.
• Kahriman N. Tosun G. Terzioglu S. Alpay Karaoğlu Ş. Yayli N. 2011. Chemical Composition and Antimicrobial Activity of the Essential Oils from the Flower, Leaf, and Stem of Senecio pandurifolius. Record of Natural Products. 5: 82-91.
• Li L. Zhao J. 2009. Determination of the Volatile Composition of Rhodobryum giganteum (Schwaegr.) Par. (Bryaceae) using Solid-Phase Microextraction and Gas Chromatography/ Mass Spectrometry (GC/ MS). Molecules. 14: 2195-2201.
• Özdemir T. Yaylı N. Cansu T.B. Volga C. Yaylı N. 2009. Essential Oils in Mosses (Brachythecium salebrosum, Eurhynchium pulchellum, and Plagiomnium undulatum) Grown in Turkey. Asian Journal of Chemistry. 21: 5505-5509.
• Özgenç Ö. Durmaz S. Çelik G. Korkmaz B. Yaylı N. 2017. Comparative phytochemical analysis of volatile organic compounds by SPME-GC-FID/MS from six coniferous and nine deciduous tree bark species grown in Turkey. South African Journal of Botany. 113: 23-28.
• Pannequin A. Tintaru A. Desjobert J.M. Costa J. Muselli A. 2017. New advances in the volatile metabolites of Frullania tamarisci. Flavour and Fragnance Journal. 32: 409-418.
• Renda G. Tosun G. Yaylı N. 2016. SPME GC/MS Analysis of Three Ornithogalum L. species from Turkey. Records of Natural Products. 10: 497-502.
• Sabovljević A. Sabovljevic M. Jockovic N. 2009. In vitro culture and secondary metabolite ısolation in Bryophytes. In: Mohan Jain S and Saxena PK (eds) Methods in Molecular Biology: Protocols for in vitro cultures and secondary metabolite analysis of aromatic and medicinal plants. Humana Press. 547: 117-128.
• Sabovljević A. Sokovic M. Glamočlija J. Ćirić A. Vujičić M. Pejin B. Sabovljević M. 2010. Comparison of extract bio-activities of in-situ and in-vitro grown selected bryophyte species. African Journal of Microbiology Research. 4: 808-812.
• Shaw A.J. Goffinet B. 2000. Bryphyte Biology. Cambridge University Press, UK.
• Saritas Y. Sonwa M.M. Iznaguen H. König W.A. Muhle H. Mues R. 2001. Volatile constituents in mosses (Musci). Phytochemistry. 57: 443-457.
• Sim-Sim M. Abreu M. Garcia C. Sergio C. Figueiredo C. 2017. Essential Oil Composition of Two Sphagnum Species Grown in Portugal and their In-Vitro Culture Establishment. Natural Product Communications. 12: 1307-1310.
• Tosun G. Yaylı B. Özdemir T. Batan N. Yaylı N. Alpay Karaoğlu Ş. 2014. Chemical Composition and Antimicrobial Activity of Essential Oils from Tortella inclinata var. Densa, T. tortusa and Pleurochaete squarrosa. Asian Journal of Chemistry. 26: 2001-2004.
• Tosun G. Yaylı B. Özdemir T. Batan N. Bozdeveci A. Yaylı N. 2015. Volatiles and Antimicrobial Activity of the Essential Oils of the Mosses Pseudoscleropodium purum, Eurhynchium striatum, and Eurhynchium angustirete Grown in Turkey. Record of Natural Products. 9: 237-242.
• Uyar G. Çetin B. 2004. A new check-list of the mosses of Turkey. Journal of Bryology. 26: 203-220.
• Üçüncü O. Cansu T.B. Özdemir T. Alpay Karaoğlu Ş. Yaylı N. 2010. Chemical composition and antimicrobial activity of the essential oils of mosses (Tortula muralis Hedw., Homalothecium lutescens (Hedw.) H. Rob., Hypnum cupressiforme Hedw., and Pohlia nutans (Hedw.) Lindb.) from Turkey. Turkish Journal of Chemistry. 34: 825-834.
• Valarezo E. Vidal V. Calva J. Jaramillo S.P. Febres J.D. Benitez A. 2018. Essential Oil Constituents of Mosses Species from Ecuador. Teop. 21: 189-197.
• Villanova P.A. 1999. Methods for Determining Bactericidal Activity of Antimicrobial Agents; Approved Guideline. NCCLS, M26-A, Vol. 19.
• Woods G.L. Brown-Elliott B.A. Desmond E.P. Hall G.S. Heifets L. Pfyffer G.E. Ridderhof J.C. Jr. Wallace R.J. Warren N.C. Witebsky F.G. 2003. Susceptibility testing of mycobacteria, nocardiae, and other aerobic actinomycetes; Approved Standard. NCCLS document, M24-A, Vol. 23.
• Zinsmeister H.D. Becker H. Eicher T. 1991. Bryophytes, a source of biologically active, naturally occurring material. Angewandte Chemie International Edition in English. 30, 130-147.
• Zinsmeister H.D. Mues R. 1987. Moose as reservoir remarkable sekundärer. Ingredients GIT Mag. Lab. 31: 499-512.