Syntrichia ruraliformis (Besch.) Mans., Hypnum andoi A.J.E.Sm. ve Platyhypnidium riparioides Dixon Etanol Ekstraktlarının HCT116 Hücre Canlılığı Üzerindeki Etkilerinin Spektroskopik Açıdan İncelenmesi

Yıl 2021, Cilt: 7 Sayı: 2, 109 - 118, 20.11.2021

Hilal Zengin Köksal Dilşad Özerkan Ergin Murat Altuner Kerem Canlı

https://doi.org/10.26672/anatolianbryology.978096

Öz

Kolon kanseri neredeyse her yıl bir milyondan fazla yeni tanıların ortaya koyulduğu ve dünyada en sık rastlanan üçüncü kanser türüdür. Her yaş grubunda görülebildiği gibi kalıtımsal kökenli değildir. Bu veriler göz önüne alındığında kanserin tedavisi için yeni ilaç kombinasyonlarının araştırılması önemli bir ihtiyaçtır. Bu çalışmada, anti-kanser aktivitesi bilinmeyen Syntrichia ruraliformis (Besch.) Mans., Hypnum andoi A.J.E.Sm, Platyhypnidium riparioides Dixon karayosunu türlerinden elde edilen ekstraktlarının HCT116 kolon kanser hücre hatlarına karşı etkileri değerlendirilmiştir. Karayosunu türlerinin etil alkol çözücüsünde elde edilen ekstrelerinden, DMSO ile stok solüsyonlar hazırlanarak, çeşitli konsantrasyonlarda sitotoksite test (MTT) deneyleri yapılmıştır. HCT116 serisinde bulunan tüm karayosunu türlerinin IC50 değerlerine göre belirlenen dozlar aynı şekilde mezenkimal kök hücrelere (MKH) uygulanmıştır. Ayrıca, Fourier transform kızılötesi (FTIR) spektroskopisi üç karayosununa maruz kalan kanser hücrelerini analiz etmek için kullanılmıştır. Sonuç olarak, karayosunu türlerinin HCT116 kolorektal kanser hücre hattına karşı yüksek oranda antiproliferatif etki gösterdiği, bunun aksine MKH hücrelerinde letal olmadığı tespit edilmiştir. Ayrıca, FTIR spektrumu ile elde edilen sonuçların tüm hücre proliferasyonu verileriyle paralel seyrettiği moleküler düzeyde de belirlenmiştir. Tüm sonuçların ışığında bu karayosunu türlerinin, biyoaktif bileşenleri belirlendiği takdirde, potansiyel kanser ilacı olma özelliğine sahip olduğu düşünülmektedir.

Anahtar Kelimeler

Briyofit, anti-proliferatif, kolorektal kanser, FTIR

Spectroscopic Investigation of the Effects of Syntrichia ruraliformis (Besch.) Mans., Hypnum andoi A.J.E.Sm. and Platyhypnidium riparioides Dixon Ethanol Extracts on HCT116 Cell Viability

Öz

Colon cancer is the third most common type of cancer in the world, with more than a million new diagnoses every year. As can be seen in all age groups, it is not inherited. Considering these data, it is an important need to research new drug combinations for the treatment of cancer. In this study, the effects of extracts obtained from Syntrichia ruraliformis (Besch.) Mans., Hypnum andoi A.J.E.Sm, Platyhypnidium riparioides Dixon bryophyte species with unknown anticancer activity against HCT116 colon cancer cell lines were evaluated. Cytotoxicity test (MTT) experiments were performed at various concentrations by preparing stock solutions with DMSO from extracts of moss species obtained in ethyl alcohol solvent. The doses determined according to the IC50 values of all moss species in the HCT116 series were applied to the mesenchymal stem cells (MSCs) in the same way. In addition, Fourier transform infrared (FTIR) spectroscopy was used to analyze cancer cells exposed to three mosses. As a result, it was determined that moss species showed a high antiproliferative effect against HCT116 colorectal cancer cell line, but on the contrary, it was not lethal in MSC cells. In addition, it was determined at the molecular level that the results obtained with the FTIR spectrum were parallel to the cell proliferation data. In the light of all the results, these Bryophyte species are considered to be potential cancer drugs if their bioactive components are determined.

Anahtar Kelimeler

Bryophyte, ant-proliferative, colorectal cancer, FTIR

Kaynakça

• Abdel-Raouf Ahmed G. Khorshid F.A.R. Kumosani T.A. 2009. FT-IR spectroscopy as a tool for identification of apoptosis-induced structural changes in A549 cells treated with PM 701. International Journal of Nano and Biomaterials. 2:1-5, 396-408.
• Antonin W. Neumann H. 2016. Chromosome condensation and decondensation during mitosis. Current opinion in cell biology. 40: 15-22.
• Asakawa Y. Ludwiczuk A. 2019. Bryophytes as a source of bioactive volatile terpenoids–A review. Food and Chemical Toxicology. 132: 110-649.
• Başer F. 2016. Ülkemizde doğal yayılışa sahip karayosunlarından Sphagnum centrale CEO Jensen ve S. capillifolium (Ehrh.) Hedw'un (Bryophyta) anti-mikrobiyal aktivitesinin belirlenmesi. Master's thesis, Adnan Menderes Üniversitesi, Fen Bilimleri Enstitüsü.
• Barth A. 2007. Infrared spectroscopy of proteins. Biochimica et Biophysica Acta (BBA)-Bioenergetics. 1767:9, 1073-1101.
• Dey A. Mukherjee A. 2015. Therapeutic potential of bryophytes and derived compounds against cancer. Journal of acute disease. 4:3, 236-248.
• Dikmen F. Keçeli T. 2017. Contributions to the Liverwort Flora (Marchantiophyta) of Gürgenli Mountain (Bayramören/Çankırı). Anatolian Bryology. 3:2, 48-57.
• Dunkhunthod B. Chitsomboon B. Thummanu K. Sittisart P. 2020. Application of FTIR Microspectroscopy to Monitor Biochemical Changes in Apoptotic Jurkat Cell Death Induced by Extract of Pseuderanthemum palatiferum Leaves. Mt Res Dev 43: 425-40.
• Emin N. Mutlu E. Güzel A.E. 2020. Determination the effectiveness of the cytotoxic analysis on the water quality assessments. TURJFAS. 8:2, 478-483.
• Erdağ A. Kürschner H. 2017. Türkiye bitkileri listesi: (Karayosunları). ANG Vakfı Yayınevi. İstanbul. Gasparri F. Muzio M. 2003. Monitoring of apoptosis of HL60 cells by Fourier-transform infrared spectroscopy. Biochemical Journal. 369:2, 239-248.
• Gieroba B. Archzewska M. Slawinska-Brych A. Rzeski W. Stepulak A. Gagos M. 2020. Prostate and breast cancer cells death induced by xanthohumol investigated with Fourier transform infrared spectroscopy. Spectrochim. Acta A Mol. Biomol. Spectrosc. 231: 118112.
• Guleken Z. Bulut H. Gültekin G.İ. Arıkan S. Yaylım İ. Hakan M.T. Sönmez D. Tarhan N. Depciuch J. 2021. Assessment of structural protein expression by FTIR and biochemical assays as biomarkers of metabolites response in gastric and colon cancer. Talanta, 231: 122-353.
• Hawrył A. Bogucka-Kocka A. Świeboda R. Hawrył M. Stebel A. Waksmundzka-Hajnos, M. 2018. Thin-layer chromatography fingerprint and chemometric analysis of selected Bryophyta species with their cytotoxic activity. JPC-Journal of Planar Chromatography-Modern TLC. 31:1, 28-35.
• Ishida K. P. Griffiths P. R. 1993. Comparison of the amide I/II intensity ratio of solution and solid-state proteins sampled by transmission, attenuated total reflectance, and diffuse reflectance spectrometry. Applied spectroscopy. 47:5, 584-589.
• Kırmacı M. Agcagil E. 2018. The bryophyte flora of Fethiye Babadağ (Muğla/Turkey). Anatolian Bryology. 4:1, 17-30.
• McLean M.H. Murray G.I. Stewart K.N. Norrie G. Mayer C. Hold G.L. El-Omar E.M. 2011. The inflammatory microenvironment in colorectal neoplasia. PLoS One. 6:1, e15366.
• Mežaka A. Znotiņa V. 2006. Epiphytic bryophytes in old growth forests of slopes, screes and ravines in north-west Latvia. Acta Universitatis Latviensis. 710: 103-116.
• Mežaka A. Brūmelis G. Piterāns A. 2008. The distribution of epiphytic bryophyte and lichen species in relation to phorophyte characters in Latvian natural old-growth broad leaved forests. Folia Cryptogamica Estonica. 44: 89-99.
• Özerkan D. Erol A. Altuner E.M Canlı K. Kuruca DS. 2021. Some Bryophytes Trigger Cytotoxicity of Stem Cell-like Population in 5-Fluorouracil Resistant Colon Cancer Cells. Nutrition and Cancer, 1-11.
• Parizadeh S.M. Jafarzadeh-Esfehani R. Hassanian SM. Parizadeh S.M.R. Vojdani S. Ghandehari M. Asgharzadeh F. 2019. Targeting cancer stem cells as therapeutic approach in the treatment of colorectal cancer. The international journal of biochemistry & cell biology. 110: 75-83.
• Saxena DK. 2004. Uses of bryophytes, Resonance. 9:6, 56-65.
• Sahu R.K. Mordechai S. Manor E. 2008. Nucleic acids absorbance in mid IR and its effect on diagnostic variates during cell division: A case study with lymphoblastic cells. Biopolymers: Original Research on Biomolecules. 89:11, 993-1001.
• Serdiuk V. Shogren K. Kovalenko T. Rasulev B. Yaszemski M. Maran A. Voronov A. 2020. Detection of macromolecular inversion–induced structural changes in osteosarcoma cells by FTIR microspectroscopy. Anal. Bioanal. Chem. 412: 7253-62.
• Singh S. Srivastava K. 2013. Bryophytes as Green Brain: Unique and Indispensable Small Creature. International Journal of Pharmaceutical Sciences Review and Research. 23:2, 28-35.
• Spjut R.W. Suffness M. Cragg G.M. Norris D.H. 1986. Mosses, liverworts, and hornworts screened for antitumor agents. Economic Botany. 40:3, 310.
• Yayıntaş O.T. Yılmaz S. Sökmen M. 2019. Determination of antioxidant, antimicrobial and antitumor activity of bryophytes from Mount Ida (Çanakkale, Turkey). Indian Journal of Traditional Knowledge (IJTK). 18:2, 395-401.
• Yetgin A. Canlı K. Altuner E.M. 2018. Comparison of antimicrobial activity of Allium sativum cloves from China and Taşköprü, Turkey. Advances in pharmacological sciences. Article ID 9302840.
• Zelig U. Kapelushnik J. Moreh R. Mordechai S. Nathan I. 2009. Diagnosis of cell death by means of infrared spectroscopy. Biophysical journal. 97:7, 2107-2114.