Investigating Seasonal Variation in The Phytochemical and Antioxidant Capacities of Different Sphagnum Taxa

Yıl 2024, Cilt: 10 Sayı: 2, 67 - 68, 05.12.2024

Tülay Aşkın Çelik , Özlem Sultan Aslantürk , Gözde Aslan , Mesut Kırmacı

https://doi.org/10.26672/anatolianbryology.1521625

Öz

This study investigated the phytochemical content and antioxidant activities of four Sphagnum taxa (S. centrale, S. palustre, S. teres and, S. auriculatum) collected from the Black Sea region of Turkey during two different seasons. The phytochemical groups in the methanol extracts of Sphagnum taxa were identified using qualitative screening methods. The total phenol content of these taxa was quantitatively determined using Folin-ciocalteu reagent with gallic acid equivalents as the standard their antioxidant activities were evaluated by 1,2-diphenyl-1-picrylhydrazyl free radical scavenging ability (DPPH), the CUPRAC test, and phosphomolybdenum assay. Qualitative phytochemical screening of the methanol extracts showed that phenols, tannins, and saponins were present in the extracts, whereas alkaloids and flavonoids were absent. The highest DPPH free radical scavenging activity was observed in 100 µgmL-1 S. teres methanol extract (74.47±0.001% in the first season, September 2021 and 75.37±0.002% in the second season, May 2022). The highest total antioxidant capacity was found in 100 µgmL-1 S. palustre extract (81.00±0.027% in the first season and 84.87±0.002% in the second season). The results of our experiment showed that Sphagnum taxa collected in spring had higher antioxidant activity than those collected in autumn.

Anahtar Kelimeler

Antioxidants, biological activity, bryophytes, seasonal changes, secondary metabolites

Etik Beyan

This research did not involve human or animal subjects and therefore does not require ethical approval.

Destekleyen Kurum

Aydın Adnan Menderes University Scientific Research Foundation

Proje Numarası

FEF-21013

Teşekkür

This work was supported by the Aydın Adnan Menderes University Scientific Research Foundation (Project No: FEF-21013).

Farklı Sphagnum Türlerinin Fitokimyasal ve Antioksidan Kapasitelerindeki Mevsimsel Değişimin İncelenmesi

Öz

Bu çalışmada, Türkiye'nin Karadeniz bölgesinden iki farklı mevsimde toplanan dört Sphagnum taksonunun (S. centrale, S. palustre, S. teres ve S. auriculatum) fitokimyasal içeriği ve antioksidan aktiviteleri araştırılmıştır. Sphagnum taksonlarının metanol ekstraktlarındaki fitokimyasal gruplar kalitatif tarama yöntemleri kullanılarak tanımlanmıştır. Bu taksonların toplam fenol içeriği, standart olarak gallik asit eşdeğerleri ile Folin-ciocalteu reaktifi kullanılarak kantitatif olarak belirlenmiş, antioksidan aktiviteleri 1,2-difenil-1-pikrilhidrazil serbest radikal süpürme yeteneği (DPPH), CUPRAC testi ve fosfomolibden deneyi ile değerlendirilmiştir. Metanol ekstrelerinin kalitatif fitokimyasal taraması, ekstrelerde fenollerin, tanenlerin ve saponinlerin bulunduğunu, alkaloidlerin ve flavonoidlerin ise bulunmadığını göstermiştir. En yüksek DPPH serbest radikal süpürme aktivitesi 100 µgmL-1 S. teres metanol ekstraktında gözlenmiştir (ilk sezon olan Eylül 2021'de %74,47±0,001 ve ikinci sezon olan Mayıs 2022'de %75,37±0,002). En yüksek toplam antioksidan kapasite 100 µgmL-1 S. palustre ekstresinde tespit edilmiştir (ilk sezonda %81,00±0,027 ve ikinci sezonda %84,87±0,002). Deneyimizin sonuçları, ilkbaharda toplanan Sphagnum taksonlarının sonbaharda toplananlara göre daha yüksek antioksidan aktiviteye sahip olduğunu göstermiştir.

Anahtar Kelimeler

antioksidan, biyolojik aktivite, briyofit, mevsimsel değişim, sekonder metabolitler

Proje Numarası

FEF-21013

Kaynakça

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