Türkiye’de Yetiştirilen Bazı Yerli Koyun Irklarında Kalpastatin (CAST) Geni Polimorfizminin PCR-RFLP Yöntemiyle Belirlenmesi

Year 2014, Volume: 20 Issue: 4, 427 - 433, 16.10.2014

Murat Balcıoğlu , Taki Karslı , Emine Şahin Zafer Ulutaş , Yüksel Aksoy

https://doi.org/10.15832/tbd.87050

Cited By: 1

Abstract

Bu çalışmada, Türkiye’de yetiştirilen 7 yerli koyun ırkında kalpastatin (CAST) gen polimorfizmi PCR-RFLP metodu kullanılarak araştırılmıştır. CAST geninin M ve N allelleri frekansları; Kangal (n= 31), İvesi (n= 26), Güney Karaman(n= 23), Akkaraman (n= 21), Morkaraman (n= 34), Karayaka (n= 33) ve Karakaş (n= 22) koyun ırklarında sırasıyla 0.92-0.08, 0.59-0.41, 0.67-0.33, 0.69-0.31, 0.87-0.13, 0.86-0.14, 0.89-0.11 olarak tespit edilmiştir. Ki-kare testi CAST geni bakımından Morkaraman, İvesi ve Karayaka populasyonlarının Hardy-Weinberg dengesinden önemli düzeyde (P<0.05)saptığını, diğer populasyonların ise Hardy-Weinberg dengesinde olduğunu göstermiştir.

Keywords

Kalpastatin geni, Polimorfzim, PCR-RFLP, Koyun

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