Cryopreservation of Mouse Sperm

Öz

Mice are preferred in scientific studies due to their small size, easy availability, rapid reproduction, and ability to create new generations in a short time. Since mice and humans have the same structure in terms of protein and DNA similarity, they are frequently used as experimental animals (Spencer, 2002). In the studies conducted, specialized mouse breeds with various characteristics have been produced. These mice are mainly used as disease models in areas such as genetic diseases, cancer, immunology, and vaccine production in humans. The genetic resources of mutant mouse breeds produced by transgenic and mutation methods and many endangered animal species can be preserved by cryopreservation. For this purpose, sperm banks and related consortia are established for many species. Sperm cryopreservation plays a crucial role in safeguarding genetic diversity both now and in the future, ensuring the preservation and enhancement of valuable genetic traits in mice used for research and practical applications. (Yıldız et al., 2007; Yamashiro and Eimei, 2012). This review aims to provide information about the cryopreservation of mouse spermatozoa.

Anahtar Kelimeler

• Mouse
• Cryopreservation
• Spermatozoa

Fare Sperminin Kriyoprezervasyonu

Öz

Fareler küçük yapıya sahip olmaları, kolay temin edilmeleri, hızlı üreyebilmeleri ve kısa sürede yeni jenerasyon oluşturabilme özelliklerinden dolayı bilimsel çalışmalarda çok fazla tercih edilmektedir. Fareler ile insanlar protein ve DNA benzerliği yönünden aynı yapıya sahip olduklarından deney hayvanı olarak sıklıkla kullanılmaktadır (Spencer, 2002). Yapılan araştırmalarda çeşitli özellikler taşıyan özelleşmiş fare ırkları üretilmiştir. Bu fareler başlıca; insanlarda genetik hastalıklar, kanser, immünoloji, aşı yapımı gibi alanlarda hastalık modeli olarak kullanılmaktadırlar. Transgenik ve mutasyon metotları ile üretilen mutant fare ırklarının ve nesli tükenmekte olan birçok hayvan türünün genetik kaynakları kriyoprezervasyon ile korunabilmektedir. Bu amaçla birçok türler için sperm bankaları ve bununla ilgili konsorsiyumlar oluşturulmaktadır. Araştırma ve uygulamalar için değerli genetik özelliklere sahip farelerin genlerini korumak ve artırmak amacıyla sperm kriyoprezervasyonu günümüzde ve gelecekte önemli bir genetik yapıyı koruma kaynağı olacaktır (Yıldız ve ark., 2007; Yamashiro ve Eimei, 2012). Bu derlemede fare sperminin kriyoprezervasyonu hakkında bilgiler verilmesi amaçlanmıştır.

Anahtar Kelimeler

• Fare
• Kriyoprezervasyon
• spermatozoa

Kaynakça

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