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The effects of extracorporeal shock waves on carrageenan-induced Achilles tendinitis in rats: a biomechanical and histological analysis

Yıl 2013, Cilt: 47 Sayı: 4, 266 - 272, 18.09.2013

Öz

Objective: The aim of this study was to investigate the influence of low-dose extracorporeal shock waves (ESW) on the healing potential of Achilles tendinitis in the rat.
Methods: The 36 adult Sprague-Dawley rats used in this study were randomly divided into four groups. Group A (n=10) were injected with carrageenan, Group B (n=10) were injected with carrageenan and received ESW, Group C (n=10) received ESW only, and Group D (n=6) was a sham group. Rats were injected with 10 microliters of 3% carrageenan or a saline solution eight times during a one-week period with a subcutaneous needle. One week following the final injection, ESW was applied at a rate of 500 impulses in 5 minutes at 2 bars (comparative to 0.09 mJ/mm2) to rats in Groups B and C. Rats were sacrificed three weeks later. Tensile strength, inflammation, and vascularity and collagen density were measured.
Results: Failure of the tendon ultimate loads was significantly lower in the study groups than in the control group (p<0.05). Collagen fiber density was higher in the control group than in the other groups (p=0.59). No other histological differences were found.
Conclusion: Low-dose ESW has a negative effect on tendon tensile strength in this animal model.

Kaynakça

  • Sharma P, Maffulli N. Biology of tendon injury: healing, modeling and remodeling. J Musculoskelet Neuronal Interact 2006;6:181-90.
  • Sharma P, Maffulli N. Tendon injury and tendinopathy: healing and repair. J Bone Joint Surg Am 2005;87:187-202. Xu ZH, Jiang Q, Chen DY, Xiong J, Shi DQ, Yuan T, et al. Extracorporeal shock wave treatment in nonunions of long bone fractures. Int Orthop 2009;33:789-93.
  • De Palma L, Gigante A, Rapali S. Physiopathology of the repair process of lesions of Achilles tendon. Foot Ankle Surg 2006;12:5-11.
  • Puddu G, Ippolito E, Postacchini F. A classification of Achilles tendon disease. Am J Sports Med 1976;4:145-50.
  • Warden SJ. Animal models for the study of tendinopathy. Br J Sports Med 2007;41:232-40.
  • Chung B, Wiley JP. Extracorporeal shockwave therapy: a review. Sports Med 2002;32:851-65.
  • Marsolais D, Côté CH, Frenette J. Neutrophils and macrophages accumulate sequentially following Achilles tendon injury. J Orthop Res 2001;19:1203-9.
  • Marsolais D, Duchesne E, Côté CH, Frenette J. Inflammatory cells do not decrease the ultimate tensile strength of intact tendons in vivo and in vitro: protective role of mechanical loading. J Appl Physiol 2007;102:11-7.
  • Tillander B, Franzén LE, Nilsson E, Norlin R. Carrageenaninduced subacromial bursitis caused changes in the rat’s rotator cuff. J Orthop Res 2001;19:441-7.
  • Di M. Biological properties of carrageenan. J Pharm Pharmacol 1972;24:89-102.
  • Wang CJ, Ko JY, Chen HS. Treatment of calcifying tendinitis of the shoulder with shock wave therapy. Clin Orthop Relat Res 2001;(387):83-9.
  • Wang CJ, Chen HS, Chen CE, Yang KD. Treatment of nonunions of long bone fractures with shock waves. Clin Orthop Relat Res 2001;(387):95-101.
  • Wang CJ, Huang HY, Pai CH. Shock wave-enhanced neovascularization at the tendon-bone junction: an experiment in dogs. J Foot Ankle Surg 2002;41:16-22.
  • Rompe JD, Kirkpatrick CJ, Küllmer K, Schwitalle M, Krischek O. Dose-related effects of shock waves on rabbit tendo Achillis. A sonographic and histological study. J Bone Joint Surg Br 1998;80:546-52.
  • Orhan Z, Cam K, Alper M, Ozturan K. The effects of extracorporeal shock waves on the rat Achilles tendon: is there a critical dose for tissue injury? Arch Orthop Trauma Surg 2004;124:631-5.
  • Orhan Z, Alper M, Akman Y, Yavuz O, Yalçiner A. An experimental study on the application of extracorporeal shock waves in the treatment of tendon injuries: preliminary report. J Orthop Sci 2001;6:566-70.
  • Rompe JD, Küllmer K, Vogel J, Eckardt A, Wahlmann U, Eysel P, et al. Extracorporeal shock-wave therapy. Experimental basis, clinical application. [Article in German]. Orthopade 1997;26: 215Chao YH, Tsuang YH, Sun JS, Chen LT, Chiang YF, Wang CC, et al. Effects of shock waves on tenocyte proliferation and extracellular matrix metabolism. Ultrasound Med Biol 2008; 34:841-52.
  • Leadbetter WB. Cell-matrix response in tendon injury. Clin Sports Med 1992;11:533-78.
  • Potenza AD. Tendon healing within the flexor digital sheath in the dog. J Bone Joint Surg Am 1962;44-A:49-64.
  • Nakama LH, King KB, Abrahamsson S, Rempel DM. Evidence of tendon microtears due to cyclical loading in an in vivo tendinopathy model. J Orthop Res 2005;23:1199-205.
  • Lake SP, Ansorge HL, Soslowsky LJ. Animal models of tendinopathy. Disabil Rehabil 2008;30:1530-41.
  • Chen YJ, Wang CJ, Yang KD, Kuo YR, Huang HC, Huang YT, et al. Extracorporeal shock waves promote healing of collagenase-induced Achilles tendinitis and increase TGFbeta1 and IGF-I expression. J Orthop Res 2004;22:854-61. Costa ML, Shepstone L, Donell ST, Thomas TL. Shock wave therapy for chronic Achilles tendon pain: a randomized placebo-controlled trial. Clin Orthop Relat Res 2005;(440):199-204. Hsu RW, Hsu WH, Tai CL, Lee KF. Effect of shock-wave therapy on patellar tendinopathy in a rabbit model. J Orthop Res 2004;22:221-7.

Sıçanlarda karragenan ile uyarılmış Aşil tendinitinde ekstrakorporeal şok dalgalarının etkinliği: Biyomekanik ve histolojik bir değerlendirme

Yıl 2013, Cilt: 47 Sayı: 4, 266 - 272, 18.09.2013

Öz

Amaç: Bu çalışmanın amacı sıçanlarda Aşil tendinitinin iyileşmesinde düşük doz ekstrakorporeal şok dalgaların etkinliğinin incelenmesi idi.
Çalışma planı: Çalışmada kullanılan 36 adet Sprague-Dawley sıçanı dört gruba ayrıldı. Grup A, karragenan enjekte edilen 10 sıçan, Grup B karragenan ve ekstrakorporeal şok dalga uygulanan 10 sıçan, Grup C ekstrakorporeal şok dalga uygulanan 10 sıçan ve Grup D kontrol grubu olarak 6 sıçandan oluşmaktaydı. Sıçanlara 10 mikrolitre %3’lük karragenan veya salin çözeltisi bir hafta içinde 8 defa subkutan iğne ile uygulandı. Son enjeksiyondan bir hafta sonra Grup B ve Grup C’deki sıçanlara 500 atım şok dalga 5 dakika süre ile 2 bar (yaklaşık 0.09 mJ/mm2) düzeyinde uygulandı. Sıçanlar üç hafta sonra sakrifiye edildi. Tendon gerim direnci, enflamasyon ve damarlanma ve kolajen yoğunlukları ölçüldü.
Bulgular: Çalışma gruplarındaki tendon gerim direncinin kontrol grubuna göre anlamlı düzeyde daha düşük olduğu belirlendi (p<0.05). Kolajen liflerin yoğunluğu kontrol grubunda diğer gruplardan daha yüksekti (p=0.59). Histolojik başka bir farklılık belirlenmedi.
Çıkarımlar: Bu hayvan modelinde düşük doz ekstrakorporeal şok dalgasının tendon gerim direncine olumsuz etkisi olduğu görülmüştür.

Kaynakça

  • Sharma P, Maffulli N. Biology of tendon injury: healing, modeling and remodeling. J Musculoskelet Neuronal Interact 2006;6:181-90.
  • Sharma P, Maffulli N. Tendon injury and tendinopathy: healing and repair. J Bone Joint Surg Am 2005;87:187-202. Xu ZH, Jiang Q, Chen DY, Xiong J, Shi DQ, Yuan T, et al. Extracorporeal shock wave treatment in nonunions of long bone fractures. Int Orthop 2009;33:789-93.
  • De Palma L, Gigante A, Rapali S. Physiopathology of the repair process of lesions of Achilles tendon. Foot Ankle Surg 2006;12:5-11.
  • Puddu G, Ippolito E, Postacchini F. A classification of Achilles tendon disease. Am J Sports Med 1976;4:145-50.
  • Warden SJ. Animal models for the study of tendinopathy. Br J Sports Med 2007;41:232-40.
  • Chung B, Wiley JP. Extracorporeal shockwave therapy: a review. Sports Med 2002;32:851-65.
  • Marsolais D, Côté CH, Frenette J. Neutrophils and macrophages accumulate sequentially following Achilles tendon injury. J Orthop Res 2001;19:1203-9.
  • Marsolais D, Duchesne E, Côté CH, Frenette J. Inflammatory cells do not decrease the ultimate tensile strength of intact tendons in vivo and in vitro: protective role of mechanical loading. J Appl Physiol 2007;102:11-7.
  • Tillander B, Franzén LE, Nilsson E, Norlin R. Carrageenaninduced subacromial bursitis caused changes in the rat’s rotator cuff. J Orthop Res 2001;19:441-7.
  • Di M. Biological properties of carrageenan. J Pharm Pharmacol 1972;24:89-102.
  • Wang CJ, Ko JY, Chen HS. Treatment of calcifying tendinitis of the shoulder with shock wave therapy. Clin Orthop Relat Res 2001;(387):83-9.
  • Wang CJ, Chen HS, Chen CE, Yang KD. Treatment of nonunions of long bone fractures with shock waves. Clin Orthop Relat Res 2001;(387):95-101.
  • Wang CJ, Huang HY, Pai CH. Shock wave-enhanced neovascularization at the tendon-bone junction: an experiment in dogs. J Foot Ankle Surg 2002;41:16-22.
  • Rompe JD, Kirkpatrick CJ, Küllmer K, Schwitalle M, Krischek O. Dose-related effects of shock waves on rabbit tendo Achillis. A sonographic and histological study. J Bone Joint Surg Br 1998;80:546-52.
  • Orhan Z, Cam K, Alper M, Ozturan K. The effects of extracorporeal shock waves on the rat Achilles tendon: is there a critical dose for tissue injury? Arch Orthop Trauma Surg 2004;124:631-5.
  • Orhan Z, Alper M, Akman Y, Yavuz O, Yalçiner A. An experimental study on the application of extracorporeal shock waves in the treatment of tendon injuries: preliminary report. J Orthop Sci 2001;6:566-70.
  • Rompe JD, Küllmer K, Vogel J, Eckardt A, Wahlmann U, Eysel P, et al. Extracorporeal shock-wave therapy. Experimental basis, clinical application. [Article in German]. Orthopade 1997;26: 215Chao YH, Tsuang YH, Sun JS, Chen LT, Chiang YF, Wang CC, et al. Effects of shock waves on tenocyte proliferation and extracellular matrix metabolism. Ultrasound Med Biol 2008; 34:841-52.
  • Leadbetter WB. Cell-matrix response in tendon injury. Clin Sports Med 1992;11:533-78.
  • Potenza AD. Tendon healing within the flexor digital sheath in the dog. J Bone Joint Surg Am 1962;44-A:49-64.
  • Nakama LH, King KB, Abrahamsson S, Rempel DM. Evidence of tendon microtears due to cyclical loading in an in vivo tendinopathy model. J Orthop Res 2005;23:1199-205.
  • Lake SP, Ansorge HL, Soslowsky LJ. Animal models of tendinopathy. Disabil Rehabil 2008;30:1530-41.
  • Chen YJ, Wang CJ, Yang KD, Kuo YR, Huang HC, Huang YT, et al. Extracorporeal shock waves promote healing of collagenase-induced Achilles tendinitis and increase TGFbeta1 and IGF-I expression. J Orthop Res 2004;22:854-61. Costa ML, Shepstone L, Donell ST, Thomas TL. Shock wave therapy for chronic Achilles tendon pain: a randomized placebo-controlled trial. Clin Orthop Relat Res 2005;(440):199-204. Hsu RW, Hsu WH, Tai CL, Lee KF. Effect of shock-wave therapy on patellar tendinopathy in a rabbit model. J Orthop Res 2004;22:221-7.
Toplam 22 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Sağlık Kurumları Yönetimi
Bölüm Deneysel Çalışma
Yazarlar

Bekir Cinar Bu kişi benim

Esra Circi Bu kişi benim

Cenk Balcik Bu kişi benim

Gulnur Guven Bu kişi benim

Sercan Akpinar Bu kişi benim

Alihan Derincek Bu kişi benim

Yayımlanma Tarihi 18 Eylül 2013
Yayımlandığı Sayı Yıl 2013 Cilt: 47 Sayı: 4

Kaynak Göster

APA Cinar, B., Circi, E., Balcik, C., Guven, G., vd. (2013). The effects of extracorporeal shock waves on carrageenan-induced Achilles tendinitis in rats: a biomechanical and histological analysis. Acta Orthopaedica Et Traumatologica Turcica, 47(4), 266-272.
AMA Cinar B, Circi E, Balcik C, Guven G, Akpinar S, Derincek A. The effects of extracorporeal shock waves on carrageenan-induced Achilles tendinitis in rats: a biomechanical and histological analysis. Acta Orthopaedica et Traumatologica Turcica. Eylül 2013;47(4):266-272.
Chicago Cinar, Bekir, Esra Circi, Cenk Balcik, Gulnur Guven, Sercan Akpinar, ve Alihan Derincek. “The Effects of Extracorporeal Shock Waves on Carrageenan-Induced Achilles Tendinitis in Rats: A Biomechanical and Histological Analysis”. Acta Orthopaedica Et Traumatologica Turcica 47, sy. 4 (Eylül 2013): 266-72.
EndNote Cinar B, Circi E, Balcik C, Guven G, Akpinar S, Derincek A (01 Eylül 2013) The effects of extracorporeal shock waves on carrageenan-induced Achilles tendinitis in rats: a biomechanical and histological analysis. Acta Orthopaedica et Traumatologica Turcica 47 4 266–272.
IEEE B. Cinar, E. Circi, C. Balcik, G. Guven, S. Akpinar, ve A. Derincek, “The effects of extracorporeal shock waves on carrageenan-induced Achilles tendinitis in rats: a biomechanical and histological analysis”, Acta Orthopaedica et Traumatologica Turcica, c. 47, sy. 4, ss. 266–272, 2013.
ISNAD Cinar, Bekir vd. “The Effects of Extracorporeal Shock Waves on Carrageenan-Induced Achilles Tendinitis in Rats: A Biomechanical and Histological Analysis”. Acta Orthopaedica et Traumatologica Turcica 47/4 (Eylül 2013), 266-272.
JAMA Cinar B, Circi E, Balcik C, Guven G, Akpinar S, Derincek A. The effects of extracorporeal shock waves on carrageenan-induced Achilles tendinitis in rats: a biomechanical and histological analysis. Acta Orthopaedica et Traumatologica Turcica. 2013;47:266–272.
MLA Cinar, Bekir vd. “The Effects of Extracorporeal Shock Waves on Carrageenan-Induced Achilles Tendinitis in Rats: A Biomechanical and Histological Analysis”. Acta Orthopaedica Et Traumatologica Turcica, c. 47, sy. 4, 2013, ss. 266-72.
Vancouver Cinar B, Circi E, Balcik C, Guven G, Akpinar S, Derincek A. The effects of extracorporeal shock waves on carrageenan-induced Achilles tendinitis in rats: a biomechanical and histological analysis. Acta Orthopaedica et Traumatologica Turcica. 2013;47(4):266-72.