Diyabeti Olan ve Diyabeti Olmayan Hastaların Kültür İdentifikasyon Yöntemleri ile Bağırsak Mikrobiyota Çeşitliliğinin Karşılaştırılması

Yıl 2025, Sayı: 12, 24 - 32, 07.08.2025

Arif İrfan Turan , Sadık Akgün , Hakan Temiz

https://doi.org/10.58252/artukluhealth.1674801

Öz

Giriş: Bu çalışmada, diyabetli ve diyabetli olmayan bireylerin bağırsak mikrobiyota çeşitliliği kültür tabanlı yöntemlerle karşılaştırılmış, elde edilen bulgular literatürle ilişkilendirilerek değerlendirilmiştir. Amaç, disbiyozun diyabet gelişimindeki olası rolünü ortaya koymaktır.
Yöntem: 25/11/2020–15/04/2021 tarihleri arasında Adıyaman Üniversitesi Hastanesi'ne dışkı analizi için gönderilen 237 örneğe kültür ve identifikasyon uygulanmış; aynı hastalardan alınan serum örneklerinde glukoz ve hemoglobin A1c düzeyleri belirlenmiştir. Veriler Statistical Package for the Social Sciences 23.0 ile analiz edilmiştir.
Bulgular: Dışkı örneklerinde en fazla üç farklı bakteri türü saptanmıştır. Tek tür bakteri üremesi diyabetli hastalarda (n=86) diyabetli olmayanlara (n=72) göre daha fazladır. İki tür üreme diyabetli olmayanlarda (n=37), diyabetlilere (n=14) kıyasla daha yaygındır. Üç tür bakteri ise sadece diyabeti olmayan iki hastada tespit edilmiştir. Ayrıca, Escherichia coli genellikle diyabetli bireylerde tek başına üremiştir.
Sonuç: Bu bulgular, bağırsak mikrobiyotasının metabolik hastalık riskini değerlendirmede potansiyel bir biyobelirteç olabileceğini göstermektedir. Ayrıca, mikrobiyota kompozisyonunun önceden analiz edilmesiyle diyabet gelişiminin önlenebileceği düşünülmektedir.

Anahtar Kelimeler

Mikrobiyota , Diyabet , Kültür , Mikroorganizma

Etik Beyan

Araştırmanın yapılabilmesi için Adıyaman Üniversitesi Girişimsel Olmayan Klinik Araştırmalar Etik Kurulu’ndan (Tarih: 20/10/2020, Sayı: 2020/9- 14) etik kurul izni alınmıştır. Bu çalışmanın hazırlanma sürecinde bilimsel ve etik ilkelere uyulduğu ve yararlanılan tüm çalışmaların kaynakçada belirtildiği beyan olunur. Bu çalışmanın hazırlanma sürecinde yapay zeka tabanlı herhangi bir araç veya uygulama kullanılmamıştır. Çalışmanın tüm içeriği, yazar(lar) tarafından bilimsel araştırma yöntemleri ve akademik etik ilkelere uygun şekilde üretilmiştir.

Destekleyen Kurum

Bu çalışma için herhangi bir bütçe desteği yoktur.

Comparison of İntestinal Bacterial Diversity in Diabetic and Non-Diabetic Patients Using Culture İdentification Methods

Öz

Introduction: Human intestinal microbiota is thought to play a role in the pathogenesis of obesity and insulin resistance. This study aimed to compare the diversity of intestinal microbiota of diabetic and non-diabetic patients using bacteriological culture and identification methods.
Methods: In this study, bacteriological culture and identification procedures were applied to 237 samples sent from various clinics to the Culture Laboratory of XXX University Education and Research Hospital for stool analysis between 2020-2021. In addition, glucose and Hemoglobin A1c values were determined in serum samples taken simultaneously from patients to whom stool samples were sent. Statistical analysis of the data obtained from the study was performed using the SPSS 23.0 package program.
Results: When the stool samples sent to our laboratory were examined in terms of species number and variety, a maximum of three types of microorganisms were detected in the media, and it was determined that diabetic patients (86 people) were numerically more than non-diabetic patients (72 people) in the media where only one (single) growth occurred. In the media with two types of microorganisms, it was observed that there were more non-diabetic patients (37 people) than diabetic patients (14 people). Three types of microorganisms were not seen in diabetic patients at all, but were detected in two people in the non-diabetic group.
Conclusion: These results suggest that microbiota can be used as a marker in determining the risk and etiopathogenesis of metabolic diseases, and the formation of diabetes can be delayed or prevented with the previously analyzed gastrointestinal system microbiota composition.

Anahtar Kelimeler

Microbiota , Diabetes , Culture , Microorganism

Etik Beyan

Ethical approval was obtained from Adıyaman University Non-Interventional Clinical Research Ethics Committee (Date: 20/10/2020, REF: 2020/9- 14) before data collection. It is declared that scientific and ethical principles were complied with during the preparation of this study and all the studies used in this study were cited in the bibliography. No artificial intelligence-based tools or applications were utilized in the preparation of this manuscript. All content was generated solely by the author(s) in adherence to scientific research methodologies and academic ethical standards.

Destekleyen Kurum

No external funding was used to support this research.

Kaynakça

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