Insecticidal Activity of Isolated Bacteria from Hyphantria cunea (Drury) (Lepidoptera: Arctiidae)

Öz

The fall webworm (Hyphantria cunea) is a polyphagous pest with numerous host plants. In the present study, the bacterial flora of H.cunea was investigated to identify new organisms that can be used as microbial control agent against the pest. Six bacteria were isolated and cultured from H. cunea. Some morphological, biochemical and other phenotypic characteristics (with API 20E, API 50 CH, API Staph and API Coryne kits) of bacterial isolates were determined. In addition, 16S rRNA gene region was sequenced. As a result of the studies conducted, bacterial isolates were identified as Lysinibacillus sphaericus (Abk1), Bacillus amyloliquefaciens (Abk2), Staphylococcus sciuri (Abk4), Kocuria palustris (Abk6), Arthrobacter arilaitensis (Abk7) and Microbacterium oxydans (Abk8). All bacterial isolates were tested for 12 days against third-fourth instar larvae of H. cunea. The highest insecticidal activity was obtained from L. sphaericus (Abk1) with 30% after application (p<0.05). These results indicate that L. sphaericus (Abk1) can be taken into account in the microbial pest control of H. cunea. In the future, further studies will be conducted by using pathogenicity enrichment strategies of L. sphaericus (Abk1) (ex. combining with other entomopathogens or insecticides) in order to increase the effectiveness on H. cunea.

Anahtar Kelimeler

• Hyphantria cunea,bacteria,biological control,pest,pathogenicity

Hyphantria cunea (Drury) (Lepidoptera: Arctiidae)’den İzole Edilen Bakterilerin İnsektisidal Aktivitesi

Öz

Amerikan Beyaz Kelebeği, Hyphantria cunea (Drury) sayısız konukçu bitkisi olan polifag bir zararlıdır.  Bu çalışmada, H. cunea’nın bakteriyal florası zararlıya karşı mikrobiyal kontrol ajanı olabilecek yeni organizmaları identifiye etmek için araştırıldı. H. cunea’dan altı bakteri izole edilerek saflaştırıldı. Bakteriyal izolatların morfolojik, biyokimyasal, diğer fenotipik özellikleri (API 20E, API 50 CH, API Staph ve API Coryne) belirlendi. İlave olarak, 16S rRNA gen sekans analizi de yapıldı. Yapılan çalışmalar sonucunda izolatlar; Lysinibacillus sphaericus (Abk1), Bacillus amyloliquefaciens (Abk2), Staphylococcus sciuri (Abk4), Kocuria palustris (Abk6), Arthrobacter arilaitensis (Abk7) ve Microbacterium oxydans (Abk8) olarak tanımlandı. Bütün bakteriler H. cunea’nın üçüncü-dördüncü gömlek larvalarına karşı oniki gün boyunca test edildi. En yüksek insektisidal aktivite %30 ile L. sphaericus (Abk1)’dan elde edildi (p<0.05). Bu sonuçlar, L. sphaericus (Abk1)’un, H. cunea’nın mikrobiyal mücadelesinde dikkate alınabileceğini göstermiştir. Gelecekte, L. sphaericus (Abk1)’un patojenite zenginleştirme stratejileri (diğer entomopatojelerle veya insektisitlerle kombine edilmesi gibi) kullanılarak H. cunea üzerindeki etkinliğinin artırılması yönünde çalışmalar yapılacaktır.

Anahtar Kelimeler

• Hyphantria cunea,bakteri,biyolojik kontrol,zararlı,patojenite

Kaynakça

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