Green synthesis of silver nanoparticles using Abelmoschus esculentus leaf and antimicrobial effects on some food pathogens

Öz

In this study, silver nanoparticles (AgNPs) were synthesized using Abelmoschus esculentus (AE) leaf extract in a low cost, environmentally friendly and easy way. According to the ultraviolet-visible (UV-vis.) spectrophotometer analysis data, the nanocrystals showed a characteristic peak at 431 nm. Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), and energy dispersive X-Ray Spectroscopy (EDX) analyzes revealed that the morphological structures of the biosynthesized AgNPs were spherical. According to the results of XRD analysis, it was seen that the crystal structures of AgNPs were cubic. The size of the nanoparticles was calculated as 19.05 nm by the Debye-Scherrer equation. The zeta potential of the synthesized nanomaterial was -9.83 mV and the zeta size was 142.2 nm. The Minimum Inhibitory Concentration (MIC) values (mg/mL) of AgNPs on the food pathogens Staphylococcus aureus ATCC 29213, Escherichia coli ATCC25922, Bacillus subtilis ATCC11774, Pseudomonas aeruginosa ATCC27833 and Candida albicans were 0.125, 0.50, 0.0312, 0.125 and 0.125 respectively

Anahtar Kelimeler

• AgNPs
• TEM
• EDX
• XRD
• Food Pathogens

Abelmoschus esculentus yaprağı kullanılarak gümüş nanopartiküllerin yeşil sentezi ve bazı gıda patojenleri üzerindeki antimikrobiyal etkileri

Öz

Bu çalışmada, Abelmoschus esculentus (AE) yaprağı özütü kullanılarak düşük maliyetli, çevre dostu ve kolay bir şekilde gümüş nanopartiküller (AgNP’ler) sentezlendi. Ultraviyole-görünür (UV-vis.) Spektrofotometre analiz verilerine göre, nanokristaller 431 nm'de karakteristik bir tepe noktası gösterdi. Taramalı Elektron Mikroskobu (SEM), Transmisyon Elektron Mikroskobu (TEM) ve enerji dağılımlı X-Işını Spektroskopisi (EDX) analizleri, biyolojik yolla sentezlenen AgNP'lerin morfolojik yapılarının küresel olduğunu ortaya koydu. XRD analizi sonuçlarına göre AgNP'lerin kristal yapılarının kübik olduğu görüldü. Nanopartiküllerin boyutu, Debye-Scherrer denklemi ile 19.05 nm olarak hesaplandı. Sentezlenen nanomalzemenin zeta potansiyeli -9.83 mV ve zeta boyutu da 142.2 nm olarak ölçüldü. AgNP'lerin, gıda patojenlerinden Staphylococcus aureus ATCC 29213, Escherichia coli ATCC25922, Bacillus subtilis ATCC11774, Pseudomonas aeruginosa ATCC27833 ve Candida albicans üzerindeki Minimum İnhibitör Konsantrasyonu (MİK) değerlerinin (mg/mL) sırasıyla 0.125, 0.50, 0.0312, 0.125 ve 0.125 düzeyinde olduğu tespit edildi.

Anahtar Kelimeler

• AgNP’ler
• TEM
• EDX
• XRD
• Gıda Patojenleri

Kaynakça

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