The Correlation between Force/Power Related Gene Polymorphisms and Explosive Power in Elite Turkish Female Volleyball Players

Yıl 2024, Cilt: 7 Sayı: 4, 641 - 654, 28.12.2024

Esin Ergin , Nurten Dinç , Selda Yücel , Tahir Atik , Esra Işık

https://doi.org/10.38021/asbid.1545958

Öz

This study aims to determine the correlation between gene polymorphisms thought to be related to force and power and explosive power in elite Turkish female volleyball players. Sixty-eight female volleyball players (age: 22.13±4.01 years, weight: 66.58±8.72 kg, height: 180.04±7.01 cm, body mass index: 20.71±4,4 kg/m2)playing in the Turkish Women's Volleyball Super League and 1st Leagues, 14% of which are national athletes and 94 college student who doesn't exercise regularly (age: 19.79±1.40 years, weight: 58.22±9.49 kg, height: 167.57±6.84 cm, body mass index: 20.70± 2.5 kg/m2) participated in the study. 2 ml venous blood sample was taken from forearm from participants, and DNA isolation was performed, and HIF1A (rs11549465), PPARG (rs1801282), NOS3 (rs2070744), and AMPD1 (rs17602729), gene polymorphisms were performed. Anthropometric measurements of the participants were taken. Following the anthropometric measurements, jump tests were applied to determine the explosive power. According to the statistical analysis results, no statistically significant difference was found between vertical jump and countermovement jump test results according to genotypes of gene polymorphisms of the volleyball players in the experimental group and the participants in the control group. In conclusion, no correlation was established between gene polymorphisms of HIF1A (rs11549465), PPARG (rs1801282), NOS3 (rs2070744), and AMPD1 (rs17602729), and explosive power among elite Turkish female volleyball players.

Anahtar Kelimeler

Volleyball, explosive power, Gene

Proje Numarası

Besyo-18001

Elit Türk Kadın Voleybolcularda Kuvvet/Güç ile Bağlantılı Gen Polimorfizmleri ve Patlayıcı Kuvvet Arasındaki İlişki

Öz

Bu çalışma elit Türk kadın voleybolcularda kuvvet ve güç ile bağlantılı olduğu düşünülen gen polimorfizmleri ile patlayıcı kuvvet arasındaki ilişkiyi belirleyebilmek amacıyla yapılmıştır. Çalışmaya Türkiye Kadınlar Voleybol süper lig ve birinci liginde oynayan, %14’ü milli sporculardan oluşan 68 kadın voleybolcu (22,13±4,01 yıl, 180,04±7,01 cm, 66,58±8,72kg, 20,71±4,4 kg/cm2) ve 94 düzenli egzersiz yapmayan üniversite öğrencisi (19,79±1,40 yıl 167,57±6,84 cm, 58,22±9,49kg, 20,70±2,5 kg/cm2) katılmıştır. Katılımcılardan ön koldan 2 ml. venöz kan örneği alınarak DNA izolasyonu yapılmış ve NOS3 (rs2070744), AMPD1 (rs17602729), PPARG (rs1801282) ve HIF1A (rs11549465) gen polimorfizmleri analizleri gerçekleştirilmiştir. Katılımcıların antropometrik ölçümleri alınmıştır. Antropometrik ölçümlerin ardından patlayıcı kuvveti belirlemek amacıyla statik dikey sıçrama ve yaylanarak sıçrama testleri uygulanmıştır. İstatistiksel analiz sonuçlarına göre deney ve kontrol grubu ile tüm katılımcılarda NOS3 (rs2070744), AMPD1 (rs17602729), PPARG (rs1801282) ve HIF1A (rs11549465) gen polimorfizmlerindeki genotip dağılımlarına bakıldığında istatistiksel olarak bir farka rastlanmamıştır. Ayrıca; deney grubunu oluşturan voleybolcular ve kontrol grubunu oluşturan katılımcıların NOS3 (rs2070744), AMPD1 (rs17602729), PPARG (rs1801282) ve HIF1A (rs11549465) gen polimorfizmlerindeki genotiplere göre dikey sıçrama ve yaylanarak sıçrama test sonuçları arasında istatistiksel olarak anlamlı farka rastlanmamıştır. Sonuç olarak; elit Türk kadın voleybolcularda NOS3 (rs2070744), AMPD1 (rs17602729), PPARG (rs1801282) ve HIF1A (rs11549465) gen polimorfizmleri ve patlayıcı kuvvet arasında bir ilişkiye rastlanmamıştır.

Anahtar Kelimeler

Voleybol,, patlayıcı kuvvet,, gen,

Destekleyen Kurum

AYDIN ADNAN MENDERES ÜNİVERSİTESİ BİLİMSEL ARAŞTIRMA PROJELERİ BİRİMİ

Proje Numarası

Besyo-18001

Kaynakça

• Ahmetov, I. I., Hakimullina, A. M., Lyubaeva, E. V., Vinogradova, O. L., & Rogozkin, V. A. (2008-a). Effect of HIF1A gene polymorphism on human muscle performance. Bulletin of Experimental Biology and Medicine, 146(3), 351-353. https://doi.org/10.1007/s10517-008-0291-3
• Ahmetov, I. I., Mozhayskaya, I. A., Lyubaeva, E. V., Vinogradova, O. L., & Rogozkin, V. A. (2008-b). PPARG Gene polymorphism and locomotor activity in humans. Bulletin of Experimental Biology and Medicine, 146(5), 630-632. https://doi.org/10.1007/s10517-009-0364-y
• Ahmetov, I. I., & Fedotovskaya, O. N. (2015). Current progress in sports genomics. Advances in Clinical Chemistry, 70, 247-314. https://doi.org/10.1016/bs.acc.2015.03.003
• Atanasov, P., Djarova, T., Kalinski, M., Petrov, L., Kaneva, R., Mugandani, S., ... & Jemni, M. (2015). ACTN3 and AMPD1 polymorphism and genotype combinations in Bulgarian athletes performing Wingate test. Journal of Sports Science, 3(3), 1-10. http://dx.doi.org/10.17265/2332-7839/2015.01.001
• Buxens, A., Ruiz, J. R., Arteta, D., Artieda, M., Santiago, C., González‐Freire, M., ... & Lucia, A. (2011). Can we predict top‐level sports performance in power vs endurance events? A genetic approach. Scandinavian Journal Of Medicine & Science in Sports, 21(4), 570-579. https://doi.org/10.1111/j.1600-0838.2009.01079.x
• Cieszczyk, P., Eider, J., Arczewska, A., Ostanek, M., Leonska-Duniec, A., Sawczyn, S., ... & Sygit, K. (2011-a). The HIF1A gene Pro582Ser polymorphism in polish power-orientated athletes. Biology of Sport, 28(2), 111. http://dx.doi.org/10.5604/945117
• Ciȩszczyk, P., Eider, J., Ostanek, M., Leońska-Duniec, A., Ficek, K., Kotarska, K., & Girdauskas, G. (2011-b). Is the C34T polymorphism of the AMPD1 gene associated with athlete performance in rowing?. International Journal of Sports Medicine, 32(12), 987-991. https://doi.org/10.1055/s-0031-1283186
• Drozdovska, S. B., Dosenko, V. E., Ilyin, V. N., Filippov, M. M., & Kuzmina, L. M. (2009). Allelic polymorphism of endothelial NO-synthase (eNOS) association with exercise-induced hypoxia adaptation. Baltic Journal of Health and Physical Activity, 1(1), 1. http://dx.doi.org/10.2478/v10131-009-0001-1
• Drozdovska, S. B., Dosenko, V. E., Ahmetov, I. I., & Ilyin, V. (2013). The association of gene polymorphisms with athlete status in Ukrainians. Biology of Sport, 30(3), 163-167. https://doi.org/10.5604/20831862.1059168
• Döring, F., Onur, S., Fischer, A., Boulay, M. R., Pérusse, L., Rankinen, T., ... & Bouchard, C. (2010). A common haplotype and the Pro582Ser polymorphism of the hypoxia-inducible factor-1α (HIF1A) gene in elite endurance athletes. Journal of Applied Physiology, 108(6), 1497-1500. https://doi.org/10.1152/japplphysiol.01165.2009
• Eynon, N., Alves, A. J., Meckel, Y., Yamin, C., Ayalon, M., Sagiv, M., & Sagiv, M. (2010). Is the interaction between HIF1A P582S and ACTN3 R577X determinant for power/sprint performance?. Metabolism, 59(6), 861-865. https://doi.org/10.1016/j.metabol.2009.10.003
• Fedotovskaya, O. N., Danilova, A. A., & Akhmetov, I. I. (2013). Effect of AMPD1 gene polymorphism on muscle activity in humans. Bulletin of Experimental Biology and Medicine, 154(4), 489-491. https://doi.org/10.1007/s10517-013-1984-9
• Fischer, H., Esbjörnsson, M., Sabina, R. L., Strömberg, A., Peyrard-Janvid, M., & Norman, B. (2007). AMP deaminase deficiency is associated with lower sprint cycling performance in healthy subjects. Journal of Applied Physiology, 103(1), 315-322. https://doi.org/10.1152/japplphysiol.00185.2007
• Gabbasov, R. T., Arkhipova, A. A., Borisova, A. V., Hakimullina, A. M., Kuznetsova, A. V., Williams, A. G., ... & Ahmetov, I. I. (2013). The HIF1A gene Pro582Ser polymorphism in Russian strength athletes. The Journal of Strength & Conditioning Research, 27(8), 2055-2058. https://doi.org/10.1519/jsc.0b013e31827f06ae
• Gómez-Gallego, F., Ruiz, J. R., Buxens, A., Artieda, M., Arteta, D., Santiago, C., ... & Lucia, A. (2009). The− 786 T/C polymorphism of the NOS3 gene is associated with elite performance in power sports. European journal of applied physiology, 107(5), 565-569. https://doi.org/10.1007/s00421-009-1166-7
• Guilherme, J. P. L. F., Bertuzzi, R., Lima‐Silva, A. E., Pereira, A. D. C., & Lancha Junior, A. H. (2018). Analysis of sports‐relevant polymorphisms in a large Brazilian cohort of top‐level athletes. Annals of Human Genetics, 82(5), 254-264. https://doi.org/10.1111/ahg.12248
• Gronek, P., Gronek, J., Lulińska-Kuklik, E., Spieszny, M., Niewczas, M., Kaczmarczyk, M., ... & Żmijewski, P. (2018). Polygenic study of endurance‐associated genetic markers NOS3 (Glu298Asp), BDKRB2 (‐9/+ 9), UCP2 (Ala55Val), AMPD1 (Gln45Ter) and ACE (I/D) in Polish male half marathoners. Journal of Human Kinetics, 64, 87. https://doi.org/10.1515/hukin-2017-0204
• Grøntved, A., Andersen, L. B., Franks, P. W., Verhage, B., Wareham, N. J., Ekelund, U., ... & Brage, S. (2011). NOS3 variants, physical activity, and blood pressure in the European Youth Heart Study. American Journal of Hypertension, 24(4), 444-450. https://doi.org/10.1038/ajh.2010.265
• Kikuchi, N., Nakazato, K., Min, S. K., Ueda, D., & Igawa, S. (2014). The ACTN3 R577X polymorphism is associated with muscle power in male Japanese athletes. The Journal of Strength & Conditioning Research, 28(7), 1783-1789. https://doi.org/10.1519/jsc.0000000000000338
• Maciejewska-Karlowska, A., Sawczuk, M., Cieszczyk, P., Zarebska, A., & Sawczyn, S. (2013). Association between the Pro12Ala polymorphism of the peroxisome proliferator-activated receptor gamma gene and strength athlete status. PloS One, 8(6), e67172. https://doi.org/10.1371/journal.pone.0067172
• McPhee, J. S., Perez-Schindler, J., Degens, H., Tomlinson, D., Hennis, P., Baar, K., & Williams, A. G. (2011). HIF1A P582S gene association with endurance training responses in young women. European Journal of Applied Physiology, 111(9), 2339-2347. https://doi.org/10.1007/s00421-011-1869-4
• Meckel, Y., Nemet, D., Alves, A. J., Eliakim, A., & Eynon, N. (2012). The AMPD1 C34T mutation is not associated with the status of Israeli athletes. European Journal of Sport Science, 12(3), 244-248. https://doi.org/10.1080/17461391.2011.552639
• Norman, B., Mahnke-Zizelman, D. K., Vallis, A., & Sabina, R. L. (1998). Genetic and other determinants of AMP deaminase activity in healthy adult skeletal muscle. Journal of Applied Physiology, 85(4), 1273-1278. https://doi.org/10.1152/jappl.1998.85.4.1273
• Prior, S. J., Hagberg, J. M., Phares, D. A., Brown, M. D., Fairfull, L., Ferrell, R. E., & Roth, S. M. (2003). Sequence variation in hypoxia-inducible factor 1α (HIF1A): association with maximal oxygen consumption. Physiological Genomics, 15(1), 20-26. https://doi.org/10.1152/physiolgenomics.00061.2003
• Rico-Sanz, J., Rankinen, T., Joanisse, D. R., Leon, A. S., Skinner, J. S., Wilmore, J. H., ... & Bouchard, C. (2003). Associations between cardiorespiratory responses to exercise and the C34T AMPD1 gene polymorphism in the HERITAGE family study. Physiological Genomics, 14(2), 161-166. https://doi.org/10.1152/physiolgenomics.00165.2002
• Rubio, J. C., Martín, M. A., Rabadán, M., Gómez-Gallego, F., San Juan, A. F., Alonso, J. M., ... & Lucia, A. (2005). Frequency of the C34T mutation of the AMPD1 gene in world-class endurance athletes: does this mutation impair performance?. Journal of Applied Physiology, 98(6), 2108-2112. https://doi.org/10.1152/japplphysiol.01371.2004
• Ruiz, J. R., Fernández del Valle, M., Verde, Z., Díez‐Vega, I., Santiago, C., Yvert, T., ... & Lucia, A. (2011). ACTN3 R577X polymorphism does not influence explosive leg muscle power in elite volleyball players. Scandinavian Journal of Medicine & Science in Sports, 21(6), e34-e41. https://doi.org/10.1111/j.1600-0838.2010.01134.x
• Sessa, F., Chetta, M., Petito, A., Franzetti, M., Bafunno, V., Pisanelli, D., ... & Margaglione, M. (2011). Gene polymorphisms and sport attitude in Italian athletes. Genetic Testing and Molecular Biomarkers, 15(4), 285-290. https://doi.org/10.1089/gtmb.2010.0179
• Sinkeler, S. P. T., Binkhorst, R. A., Joosten, E. M., Wevers, R. A., Coerwinkei, M. M., & Oei, T. L. (1987). AMP deaminase deficiency: study of the human skeletal muscle purine metabolism during ischaemic isometric exercise. Myoadenylate Deaminase Deficiency, 59. https://doi.org/10.1042/cs0720475
• Szelid, Z., Lux, Á., Kolossváry, M., Tóth, A., Vágó, H., Lendvai, Z., ... & Merkely, B. (2015). Right ventricular adaptation is associated with the Glu298Asp variant of the NOS3 gene in elite athletes. PLoS One, 10(10), e0141680. https://doi.org/10.1371/journal.pone.0141680
• Tarnopolsky, M. A., Parise, G., Gibala, M. J., Graham, T. E., & Rush, J. W. (2001). Myoadenylate deaminase deficiency does not affect muscle anaplerosis during exhaustive exercise in humans. The Journal of Physiology, 533(3), 881-889. https://doi.org/10.1111%2Fj.1469-7793.2001.t01-1-00881.x
• Zilberman-Schapira, G., Chen, J., & Gerstein, M. (2012). On sports and genes. Recent Patents on DNA & Gene Sequences (Discontinued), 6(3), 180-188. https://doi.org/10.2174/187221512802717367