PERİODONTAL DURUMUN TÜKÜRÜK GROWTH ARREST-SPECIFIC PROTEIN 6(GAS6) DÜZEYİ ÜZERİNE ETKİSİNİN İNCELENMESİ

Nur Balcı; Arten Dyrmishi; Metin Çetin; Ali Çekici

doi:10.17567/ataunidfd.674684

PERİODONTAL DURUMUN TÜKÜRÜK GROWTH ARREST-SPECIFIC PROTEIN 6(GAS6) DÜZEYİ ÜZERİNE ETKİSİNİN İNCELENMESİ

Öz

Giriş: Periodontitis, diş destek dokularında yıkımla karakterize olan ve değişik formları bulunan kronik inflamatuar bir hastalıktır. Growth arrest-specific protein 6(Gas6), hücre proliferasyonunu, adezyonunu, migrasyonunu, apoptotik hücrelerin makrofajlar tarafından fagositozunu ve adiposit gelişimini düzenleyen; obezite, diyabetes mellitus ve aterosklerozis gibi kronik hastalıkların patogenezinde rol alan bir proteindir. Bilgimiz dahilinde literatürde Gas6 proteinin periodontal hastalık ile ilişkisi araştırılmamıştır. Bu çalışmanın amacı, Gas6 proteininin periodontal doku yıkımındaki rolünün araştırılması ve bu molekülün periodontal hastalık patogenezindeki yerinin aydınlatılmasıdır. Gereç ve Yöntem: Periodontoloji Kliniğine tedavi için başvuran ve periodontitis (evre III, derece B, generalize) tanısı konmuş sistemik olarak sağlıklı (8 kadın, 13 erkek) 20 hasta ile sistemik ve periodontal olarak sağlıklı (7 kadın, 12 erkek) 20 birey çalışmaya dahil edilmiştir. Tükürük örnekleri, klinik periodontal indekslerin ölçümleri (Sondalamada cep derinliği (SCD), sondalamada kanama indeksi (SKİ), dişeti çekilmesi (DÇ), plak indeksi (Pİ) ve klinik ataçman kaybı (KAK)) öncesinde alınmıştır. Toplanan tükürük örneklerinde Gas6 anti-enflamtuar proteini ELISA yöntemi ile incelenmiştir. Sonuçlar IBM SPSS Statistics 22 programı kullanılarak Mann Whitney U testi, Continuity (Yates) Düzeltmesi ve Ki-Kare testi ile değerlendirilmiştir. Bulgular: Periodontitis hastalarının tüm klinik periodontal indeks değerleri, sağlıklı bireylerden istatistiksel olarak anlamlı yüksek bulunmuştur (p:0.001;p<0.01). Tükürükteki Gas6 protein düzeyi periodontitisli ve sağlıklı bireyler arasında istatistiksel olarak anlamlı bir fark göstermemiştir. Sonuç: Bu çalışmanın sonuçlarına göre periodontitisli bireylerin tükürüğünde Gas6 proteini tespit edilmiştir. Ancak Gas6’nın periodontitisin patogenezinde olası bir rolünün olup olmadığı tartışmalıdır. Serum ve dişeti oluğu sıvısı örneklerinin de dahil edildiği ve anti-enflamatuar proteinler ile pro-enflamatuar sitokinler arası ilişkinin değerlendirilebileceği ileri çalışmalara ihtiyaç vardır. Anahtar Kelimeler: Gas6, Periodontitis, Tükürük Evaluation of the effect of periodontal status on saliva Growth arrest-specific protein 6(Gas6) Aim: Periodontitis is a chronic inflammatory disease which is characterized with destruction of tooth supporting tissues and have various forms. Growth arrest-specific protein 6(Gas6), is a protein that regulates cell proliferation, adhesion, migration, phagocytosis of apoptotic cells by macrophages and adipocyte development and also has roles on the pathogenesis of chronic diseases such as obesity, diabetes mellitus and atherosclerosis. To the best of our knowledge, the relationship between Gas6 protein and periodontal disease has not been investigated in the literature. The aim of this study was to investigate the role of Gas6 protein in periodontal tissue destruction and to elucidate the role of this molecule in the pathogenesis of periodontal disease. Material and Method: Twenty systemically healthy (8 female, 13 male) patients with periodontitis (stage III, grade B, generalized) admitted to the periodontology clinic for treatment and systemically and periodontally healthy 20 individuals (7 female, 12 male) were included in the study. Saliva samples were taken before the measurement of clinical periodontal indices (Probing pocket depth(PPD), bleeding on probing (BOP), gingival recession (GR), plaque index (PI) and clinical attachment loss (CAL). Gas6 anti-inflammatory protein in saliva samples was examined by ELISA method. Results were evaluated by Mann Whitney U test, Continuity (Yates) correction and Chi-Square test using IBM SPSS Statistics 22 program. Results: All clinical periodontal index values of patients with periodontitis were significantly higher than healthy subjects (p: 0.001; p <0.01). Gas6 protein level in saliva did not show a statistically significant difference between periodontitis and healthy individuals. Conclusion: According to the results of this study, Gas6 protein was detected in the saliva of periodontitis patients. However, it is controversial whether Gas6 has a possible role in the pathogenesis of periodontitis. Further studies are needed to include serum and gingival fluid samples and to evaluate the relationship between anti-inflammatory proteins and pro-inflammatory cytokines. Key Words: Gas6, Periodontitis, Saliva

Anahtar Kelimeler

Kaynakça

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Ayrıntılar

Birincil Dil

Türkçe

Konular

Diş Hekimliği

Bölüm

Araştırma Makalesi

Yazarlar

Nur Balcı
0000-0001-7986-7085
Andorra

Arten Dyrmishi Bu kişi benim
0000-0002-4422-8379
Andorra

Metin Çetin Bu kişi benim
0000-0001-9963-8407
Andorra

Ali Çekici Bu kişi benim
0000-0002-5243-0948
Andorra

Yayımlanma Tarihi

15 Temmuz 2020

Gönderilme Tarihi

7 Ekim 2019

Kabul Tarihi

14 Ocak 2020

Yayımlandığı Sayı

Yıl 2020 Cilt: 30 Sayı: 3

DOI

https://doi.org/10.17567/ataunidfd.674684

IZ

https://izlik.org/JA34PW78MA

Kaynak Göster

RIS / Bibtex

APA

Balcı, N., Dyrmishi, A., Çetin, M., & Çekici, A. (2020). PERİODONTAL DURUMUN TÜKÜRÜK GROWTH ARREST-SPECIFIC PROTEIN 6(GAS6) DÜZEYİ ÜZERİNE ETKİSİNİN İNCELENMESİ. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi, 30(3), 379-385. https://doi.org/10.17567/ataunidfd.674684

AMA

1.Balcı N, Dyrmishi A, Çetin M, Çekici A. PERİODONTAL DURUMUN TÜKÜRÜK GROWTH ARREST-SPECIFIC PROTEIN 6(GAS6) DÜZEYİ ÜZERİNE ETKİSİNİN İNCELENMESİ. Ata Diş Hek Fak Derg. 2020;30(3):379-385. doi:10.17567/ataunidfd.674684

Chicago

Balcı, Nur, Arten Dyrmishi, Metin Çetin, ve Ali Çekici. 2020. “PERİODONTAL DURUMUN TÜKÜRÜK GROWTH ARREST-SPECIFIC PROTEIN 6(GAS6) DÜZEYİ ÜZERİNE ETKİSİNİN İNCELENMESİ”. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi 30 (3): 379-85. https://doi.org/10.17567/ataunidfd.674684.

EndNote

Balcı N, Dyrmishi A, Çetin M, Çekici A (01 Temmuz 2020) PERİODONTAL DURUMUN TÜKÜRÜK GROWTH ARREST-SPECIFIC PROTEIN 6(GAS6) DÜZEYİ ÜZERİNE ETKİSİNİN İNCELENMESİ. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi 30 3 379–385.

IEEE

[1]N. Balcı, A. Dyrmishi, M. Çetin, ve A. Çekici, “PERİODONTAL DURUMUN TÜKÜRÜK GROWTH ARREST-SPECIFIC PROTEIN 6(GAS6) DÜZEYİ ÜZERİNE ETKİSİNİN İNCELENMESİ”, Ata Diş Hek Fak Derg, c. 30, sy 3, ss. 379–385, Tem. 2020, doi: 10.17567/ataunidfd.674684.

ISNAD

Balcı, Nur - Dyrmishi, Arten - Çetin, Metin - Çekici, Ali. “PERİODONTAL DURUMUN TÜKÜRÜK GROWTH ARREST-SPECIFIC PROTEIN 6(GAS6) DÜZEYİ ÜZERİNE ETKİSİNİN İNCELENMESİ”. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi 30/3 (01 Temmuz 2020): 379-385. https://doi.org/10.17567/ataunidfd.674684.

JAMA

1.Balcı N, Dyrmishi A, Çetin M, Çekici A. PERİODONTAL DURUMUN TÜKÜRÜK GROWTH ARREST-SPECIFIC PROTEIN 6(GAS6) DÜZEYİ ÜZERİNE ETKİSİNİN İNCELENMESİ. Ata Diş Hek Fak Derg. 2020;30:379–385.

MLA

Balcı, Nur, vd. “PERİODONTAL DURUMUN TÜKÜRÜK GROWTH ARREST-SPECIFIC PROTEIN 6(GAS6) DÜZEYİ ÜZERİNE ETKİSİNİN İNCELENMESİ”. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi, c. 30, sy 3, Temmuz 2020, ss. 379-85, doi:10.17567/ataunidfd.674684.

Vancouver

1.Nur Balcı, Arten Dyrmishi, Metin Çetin, Ali Çekici. PERİODONTAL DURUMUN TÜKÜRÜK GROWTH ARREST-SPECIFIC PROTEIN 6(GAS6) DÜZEYİ ÜZERİNE ETKİSİNİN İNCELENMESİ. Ata Diş Hek Fak Derg. 01 Temmuz 2020;30(3):379-85. doi:10.17567/ataunidfd.674684