TRACE ELEMENT LEVELS IN SALIVA IN SUBJECTS WITH COMPOSITE FILLING

Öz

Objective: Trace elements are agents that regulate various biological pathways. Copper and zinc are essential components of the antioxidant enzyme system. The aim of this study was to determine iron, copper, and zinc levels in saliva after application of composite resin restoration. Materials and Methods: Twenty cases of class I caries cavity (M:F ratio 8:12; age 18-25 years) were selected for the present study. Following cavity preparation, the restorative material (Filtek Z 250) was applied to the cavities in line with the manufacturer’s instructions. Unstimulated whole saliva samples were collected from before, and one hour, one day, seven days, and 30 days after restoration. Trace element saliva contents (zinc, copper, and iron) were analyzed using an atomic absorption spectrophotometer (AAS) (AAnalyst 800, Perkin Elmer, USA) with the electrothermal graphite oven technique. Repeated measures analysis of variance was used to evaluate trace element levels over time (=0.05). Results: Copper, zinc, and iron levels in saliva decreased from 0.189±0.138 µg/ml to 0.123±0.031 µg/ml, from 0.067±0.012 µg/ml to 0.060±0.013 µg/ml, and from 0.162±0.049 µg/ml to 0.126±0.032 µg/ml, respectively, at the end of 30 days. However, these changes were not statistically significant (p>0.05). Conclusions: Composite resin used in the present study did not affect trace element levels in saliva. Keywords: composite resin, copper, iron, saliva, zinc KOMPOZIT DOLGU YAPILAN BIREYLERDE TÜKÜRÜK ESER ELEMENT SEVIYESI Öz Amaç: Eser elementler, çeşitli biyolojik yolları düzenleyen maddelerdir. Bakır ve çinko antioksidan enzim sisteminin temel bileşenleridir. Bu çalışmanın amacı, kompozit rezin restorasyonu uygulandıktan sonra tükürükteki demir, bakır ve çinko seviyelerini belirlemektir. Materyal ve metot: Çalışmaya sınıf I çürük kavitesine sahip 20 adet birey dahil edildi. (E:K oranı 8:12; yaş 18-25 yıl). Kavite pereperasyonundan sonra restoratif materyal (Filtek Z 250) üretici firma talimatlarına göre uygulandı. Uyarılmamış tükürük örnekleri restorasyondan önce, restorasyondan 1 saat, 1 gün, 7 gün ve 30 gün sonra toplandı. Tükürükteki eser element miktarı elektrotermal grafit fırın tekniği ile atomik absorbsiyon spektrofotometresi (AAS) (AAnalyst 800, Perkin Elmer, USA) kullanılarak ölçüldü. Zamanla eser element miktarındaki değişim, tekrarlı ölçümler varyans analizi kullanılarak değerlendirildi (=0.05). Bulgular: Tükürükteki bakır, çinko ve demir seviyeleri 30 gün sonunda sırasıyla 0.189±0.138 µg/ml’den 0.123±0.031 µg/ml’e , 0.067±0.012 µg/ml’ den 0.060±0.013 µg/ml’ ye 0.162±0.049 µg/ml’den 0.126±0.032 µg/ml’ye düşüş göstermektedir. Ancak bu değişiklikler istatistiksel olarak anlamlı değildir (p>0.05). Sonuç: Bu çalışmada kullanılan kompozit rezin tükürük eser element seviyesini etkilememiştir. Anahtar kelimeler: bakır, çinko, demir, kompozit rezin, tükürük

Anahtar Kelimeler

• composite resin
• copper
• iron
• saliva, zinc

TRACE ELEMENT LEVELS IN SALIVA IN SUBJECTS WITH COMPOSITE FILLING

Öz

Aim: Trace elements are agents that regulate various biological pathways. Copper and zinc are essential components of the antioxidant enzyme system. The aim of this study was to determine iron, copper, and zinc levels in saliva after application of composite resin restoration. Materials and Methods: Twenty cases of class I caries cavity (M:F ratio 8:12; age 18-25 years) were selected for the present study. Following cavity preparation, the restorative material (Filtek Z 250) was applied to the cavities in line with the manufacturer’s instructions. Unstimulated whole saliva samples were collected from before, and one hour, one day, seven days, and 30 days after restoration. Trace element saliva contents (zinc, copper, and iron) were analyzed using an atomic absorption spectrophotometer (AAS) (AAnalyst 800, Perkin Elmer, USA) with the electrothermal graphite oven technique. Repeated measures analysis of variance was used to evaluate trace element levels over time (=0.05). Results: Copper, zinc, and iron levels in saliva decreased from 0.189±0.138 µg/ml to 0.123±0.031 µg/ml, from 0.067±0.012 µg/ml to 0.060±0.013 µg/ml, and from 0.162±0.049 µg/ml to 0.126±0.032 µg/ml, respectively, at the end of 30 days. However, these changes were not statistically significant (p>0.05). Conclusions: Composite resin used in the present study did not affect trace element levels in saliva. Keywords: composite resin, copper, iron, saliva, zinc KOMPOZIT DOLGU YAPILAN BIREYLERDE TÜKÜRÜK ESER ELEMENT SEVIYESI Özet Amaç: Eser elementler, çeşitli biyolojik yolları düzenleyen maddelerdir. Bakır ve çinko antioksidan enzim sisteminin temel bileşenleridir. Bu çalışmanın amacı, kompozit rezin restorasyonu uygulandıktan sonra tükürükteki demir, bakır ve çinko seviyelerini belirlemektir. Materyal ve metot: Çalışmaya sınıf I çürük kavitesine sahip 20 adet birey dahil edildi. (E:K oranı 8:12; yaş 18-25 yıl). Kavite pereperasyonundan sonra restoratif materyal (Filtek Z 250) üretici firma talimatlarına göre uygulandı. Uyarılmamış tükürük örnekleri restorasyondan önce, restorasyondan 1 saat, 1 gün, 7 gün ve 30 gün sonra toplandı. Tükürükteki eser element miktarı elektrotermal grafit fırın tekniği ile atomik absorbsiyon spektrofotometresi (AAS) (AAnalyst 800, Perkin Elmer, USA) kullanılarak ölçüldü. Zamanla eser element miktarındaki değişim, tekrarlı ölçümler varyans analizi kullanılarak değerlendirildi (=0.05). Bulgular: Tükürükteki bakır, çinko ve demir seviyeleri 30 gün sonunda sırasıyla 0.189±0.138 µg/ml’den 0.123±0.031 µg/ml’e , 0.067±0.012 µg/ml’ den 0.060±0.013 µg/ml’ ye 0.162±0.049 µg/ml’den 0.126±0.032 µg/ml’ye düşüş göstermektedir. Ancak bu değişiklikler istatistiksel olarak anlamlı değildir (p>0.05). Sonuç: Bu çalışmada kullanılan kompozit rezin tükürük eser element seviyesini etkilememiştir. Anahtar kelimeler: bakır, çinko, demir, kompozit rezin, tükürük

Anahtar Kelimeler

• composite resin
• copper, iron
• saliva, zinc

Kaynakça

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