İrrigasyon Rejimlerinin Enterococcus Faecalis Biofilmi Üzerine İn-Vitro Antibakteriyel Etkinliği

Yıl 2013, Cilt: 23 Sayı: 2, 165 - 171, 01.02.2013

Dr. Didem Atabek Yrd. Doç. Dr. Çağdaş Çınar Prof. Dr. Nurhan Öztaş Yrd. Doç. Dr. Gülçin Akca. Prof. Dr. Zek Suludere

Öz

Objectives: The aim of this study was to compare the antibacterial efficiency of 2.25% sodium hypochlorite (NaOCl), 2% chlorhexidine (CHX), and gaseous ozone to biofilm of Enterococcus faecalis ( E. faecalis) generated on cellulose nitrate membrane filters. Materials and methods: After an overnight culture of E. faecalis grown in tryptic soy (TYC) broth, an aliquot of 100µl of microorganisms was seeded onto 13.0-mm diameter cellulose nitrate membrane filters (Sartorius®, UK) and incubated for 48 to 72 h at 37ºC in an aerobic atmosphere. Twenty membranes were used for each test agent. Then, biofilm formation test agents, gaseous ozone, and 0.9% NaCl (positive control) were applied at time intervals. Application periods for NaOCl and CHX were 5 to 10 minutes, whereas ozone application with Ozonytronx (Bizonix, GmbH, Germany) was 80 to 120 seconds. Next, from each of the samples, 100µl aliquots were put onto the TYC agar plates. Incubation colonies were calculated as colony forming units (CFU/mL) per membrane and observed by scanning electron microscopy (SEM). Data were analyzed using the Wilcoxon test, KruskalWallis test, and Mann-Whitney U-test at p Results: All of the irrigation regimens disgregated and removed the biofilm compared to the positive control (p CHX caused a significantly higher degree of biofilm disgregation than ozone application (p Conclusions: The irrigation regimen of 2.25% NaOCl and 2% CHX was more effective in eliminating biofilm formation of E. faecalis. However, the long-term irrigation regimen of ozone was found to be a successful alternative for endodontic treatment.Key-words: Biofilm, Ozone, Enterococcus faecalis

Anahtar Kelimeler

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In-Vitro Antibacterial Efficiency Of Irrigation Regimens Against Biofilm Of Enterococcus Faecalis

Öz

Amaç: Çalışmanın amacı % 2,25 sodyum hipoklorid (NaOCl), %2 klorheksidin (CHX) ve ozon gazının selüloz nitrat membran filtreler üzerinde oluşturulan Enterococcus faecalis ( E. faecalis) biyofilmi üzerindeki antibakteriyal etkinliğini karşılaştırmaktır. Materyal method: Triptik soy (TYC) besiyerinde bir gece E. faecalis kültüre edilerek, 100 µl mikroorganizma sıvısı 13,0-mm çapında selüloz nitrat membran filtreler (Sartorius®, İngiltere) üzerine ekildi ve 48-72 saat 37ºC’de aerobic bir atmosferde inkübe edildi. Her test ajanı için 20 membran kullanıldı. Test ajanları, ozon gazı ve %0,9 NaCl (pozitif kontrol) zaman aralıklarında biyofilm formasyonuna uygulandı. NaOCl ve CHX için uygulama periyotları 5 ve 10 dak. iken; Ozonytronx (Bizonix, GmbH, Almanya) cihazı ile ozon gazı 80 ve 120 saniye uygulandı. Her örnekten 100µl sıvı TYC agarlara konuldu. İnkübasyon kolonileri membran yüzeyinde koloni oluşturan birimler (CFU/mL) olarak hesaplandı ve SEM ile gözlemlendi. Veriler Wilcoxon, Kruskal-Wallis ve Mann-Whitney Utestleri kullanılarak p<0,05 güven aralığında değerlendirildi. Bulgular: Pozitif kontrol grubu ile kıyaslandığında tüm irrigasyon rejimleri biyofilmi bozuntuya uğrattı ( p CHX ozon grubundan istatistiksel olarak anlamlı farklılık yaratacak düzeyde yüksek biyofilm bozunumu oluşturdu (p Sonuç: %2,25 NaOCl ve %2 CHX rejimleri E. faecalis biyofilm formasyonunun eliminasyonunda daha etkilidir. Öte yandan daha uzun süreli ozon gazı uygulamaları endodontik tedavi için alternatif bir irrigasyon rejimi olabilir.

Anahtar Kelimeler

Biofilm, Ozon, Enterococcus faecalis

Kaynakça

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