Oxidative Stress in Akkaraman Ewes with Seropositive for Schmallenberg Virus

Öz

The aim of this study was to determine oxidant-antioxidant status by evaluating some oxidative stress parameters (nitric oxide (NO), malondialdehyde (MDA), total antioxidant status (TAS), total oxidant status (TOS), catalase activity) in clinically healthy Akkaraman ewes with seropositive for Schmallenberg virus (SBV). The study consisted of four positive, six suspected and seven negative-control blood sera in terms of specific antibody for SBV by using commercial ELISA kits. Even though there were no significant statistical differences (P>0.05), the highest NO level was in sera with SBV specific antibody positive while the lowest TOS level was in sera with SBV specific antibody negative. The values of TAS in sera were very similar in the groups (P=0.981). While the MDA levels of sera with SBV specific antibody positive and suspected were similar, the statistical difference was found between the MDA level of sera with SBV specific antibody positive and negative (P=0.021). The catalase activity of sera with SBV specific antibody positive was lower (P=0.030) than the catalase activity of sera with SBV suspected whereas the catalase activities in positive and negative groups and the catalase activities in suspected and negative groups were similar. In conclusion, it was determined that MDA level was affected in Akkaraman ewes that are SBV specific antibody positive without clinical symptoms.

Anahtar Kelimeler

• Oxidative stress,Schmallenberg virus,sheep

Schmallenberg Virüs Seropozitif Akkaraman Koyunlarda Oksidatif Stres

Öz

Bu çalışmanın amacı Schmallenberg virüs (SBV) seropozitif belirlenen klinik olarak sağlıklı Akkaraman koyunlarda bazı oksidatif stres parametrelerinin (nitrik oksit (NO), malondialdehid (MDA), total antioksidan kapasite (TAK), total oksidan kapasite (TOK), katalaz aktivitesi) değerlendirilmesiyle oksidan-antioksidan durumun belirlenmesidir. Çalışmanın materyalini, ELISA yöntemiyle SBV özgül antikorları yönünden 4 pozitif, 6 şüpheli ve 7 negatif-kontrol kan serumu oluşturdu. Gruplar arasında istatistiksel bir fark olmamasına rağmen (P>0.05) en yüksek NO seviyesi SBV seropozitif serumlarındayken, en düşük TOK seviyesi SBV negatif serumlarda bulundu. Gruplardaki TAS değerleri oldukça benzerdi (P=0.981). SBV seropozitif ve şüpheli serum MDA seviyeleri benzerken SBV seropozitif ve negatif serumların MDA seviyeleri istatistiki olarak farklı bulundu (P=0.021). SBV seropozitif olan serumların katalaz aktiviteleri, SBV şüpheli serumların katalaz aktivitelerinden daha düşük (P=0.030) iken pozitif ve negatif grupların katalaz aktiviteleri ile şüpheli ve negatif grupların katalaz aktiviteleri benzer bulundu. Sonuç olarak; hastalığa ait klinik semptom bulunmayan SBV seropozitif Akkaraman ırkı hayvanlarda MDA seviyesinin etkilendiği belirlendi.

Anahtar Kelimeler

• Koyun,oksidatif stres,Schmallenberg virüs

Kaynakça

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