The Effects of Dietary Vitamin E and Organic Selenium on the Levels of Some Bioelements in Tissues of Laying Hens

Abstract

This experiment was conducted to evaluate the effects of dietary vitamin E (α-tocopherol acetate), organic selenium (selenomethionine) and their combination on some bioelements in some tissues of laying hens. Ninety-six white Lohman LSL laying hens aged of 24 weeks were randomly divided into 4 equal groups. The groups were fed with the diets consisting of basal diet (Control), basal diet + 250 mg/kg Vit-E (Trial-I), basal diet + 0.9mg/kg Se (Trial-II) and basal diet + 250 mg/kg Vit-E + 0,9 mg/kg Se (Trial-III), respectively. At the end of the experiment, 12 laying hens from each group were sacrificed, and aforementioned tissues were collected and stored at -20 ºC until analyzes. In tissue samples bioelements levels were determined by ICP-MS. It was found out that when the treatment and control groups were compared in terms of bioelements in some important tissues while Se, Zn and Fe levels increased (P<0.05) and the levels of Cu decreased in the tissues of treatment groups (P<0.05). In addition, the levels of Ca and Mg were not affected by treatment in present study. Organic selenium and vitamin E, consideration of antagonist and synergistic effects of bioelement levels changes in tissues.

Keywords

• Bioelements,Laying hen,Organic selenium,Tissue samples,Vitamin E

Diyetsel Vitamin E ve Organik Selenyumun Yumurta Tavuğu dokularındaki Bazı Biyoelement Düzeyleri Üzerine Etkileri

Öz

Bu çalışma, diyetsel vitamini E (a-tokoferol asetat), organik selenyum (selenomethionine) ve bunların kombinasyonlarının yumurta tavuklarının bazı dokularında bazı biyoelementlerin düzeyleri üzerine etkilerini değerlendirmek amacıyla yapıldı. Çalışmada, 96 adet beyaz Lohman yumurta tavuğu, eşit sayıda 4 gruba ayrıldı. Gruplar sırasıyla bazal yem (Kontrol), bazal yem + 250 mg/kg Vit-E (D-I), bazal yem + 0.9 mg/kg Se (DII) ve bazal yem + 250 mg/kg Vit-E+ 0.9 mg/ kg Se (D-III) içeren rasyonlarla beslendi. Deneme sonunda, her gruptan 12 hayvan kesildi, belirtilen dokular alındı, analiz edilinceye kadar -20 ºC'de saklandı ve biyoelement düzeyleri ICP-MS ile analiz edildi. Deneme gruplarının dokularındaki Se, Zn ve Fe düzeyleri kontrol grubuna göre önemli derecede artarken (P<0.05), Cu düzeylerinin azaldığı (P<0.05), Ca ve Mg düzeylerinin ise muameleden etkilenmediği bulundu. Organik selenyumun ve vitamin E’nin, dokularda biyoelement düzeylerindeki değişmelerin oluşturacağı antagonist ve sinerjik etkilerinin dikkate alınması önerilmektedir.

Anahtar Kelimeler

• Bioelements,Tissue Samples,Laying Hen,Vitamin E,Organik selenyum

Kaynakça

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