Uzun Transporta Bağlı Aygırların Strese Tepkileri: Tam Kan Sayımı ve Serum Biyokimya Varyasyonları

Öz

Bu çalışmada, transportun atlarda bazı stres göstergelerine etkisini belirlemek amaçlanmıştır. 7 Safkan, 2 Arap, 1 Hollanda Yarım Kan ve 1 Fresyan olmak üzere 3-12 yaş arası 11 olgun at (aygır) kayıt edildi.  Transport öncesi ve sonrasında, otomatik kan sayımı ile beyaz kan hücresi sayımı ve diğer ilgili parametreler belirlendi. İlgili biyolojik belirteçlerin serum aktiviteleri bir otoanalizör kullanılarak belirlendi. Na, K, Cl ve Ca düzeyleri de otoanalizör kullanılarak ölçüldü. Ortalama Platelet (PLT) ve Oratalama Hemoglobin Konsantrasyonu (MCHC) Total Lökosit (WBC) sayıları çalışma süresince sağlıklı atlar için bir farklılık göstermezken, Alyuvarlar (RBC), Hemoglobin (Hb) ve Hematokrit (Hct) düzeyleri ulaşımdan sonra anlamlı artışlar sergiledi (P<0.05). Günlük ortalama glikoz konsantrasyonu transporttan sonra artış gösterdi (P<0.05). Ortalama total protein, albümin, Aspartat aminotransferaz (AST) ve Kreatinin kinaz (CK) düzeyleri, transportun ardından 2. günde artmaktadır (P<0.05). Ortalama kan üresi azotu, kreatinin, total bilirubin, Alanin aminotransferaz (ALT), Alkalen fosfataz (ALP), Laktat dehidrogenaz (LDH) ve Gama-gulutamil Transferaz (GGT), çalışma boyunca önemli değişiklikler göstermedi. Transporttan sonra ortalama Sodyum (Na), Potasyum (K) ve Klor (Cl) düzeyleri değişti (P<0.05), nakil sonrası Kalsiyum (Ca) düzeyleri anlamlı düşüş gösterdi (P <0.05). 

Anahtar Kelimeler

• Aygır,Biyokimya,Hematoloji,Stres,Transport

Stress Responses of Stallions During Transport Period: The Variations of Complete Blood Count and Serum Biochemistry

Abstract

In the present study the objective was to determine the effect of one transport journey on some stress indicators in horses. 11 adult stallion (all stallion) age 3-12 years including 7 Thoroughbred, 2 Arabian, 1 Dutch Warmblood, and 1 Friesian were enrolled. White blood cell counts and other relevant parameters were determined by an automated cell counter before and after transportation. Serum activities of relevant biomarkers were determined by use of an autoanalyzer. Serum cortisole level was detected by a microplate immunoassay technique. Sodium (Na), Potassium (K) and Clor (Cl) and Calsium (Ca) levels were also measured by use of autoanalyser. Mean Platelet (PLT), and Mean corpuscular hemoglobin concentration  (MCHC) White Blood Cell (WBC) counts did not exhibit a variation for healthy horses during the study whereas RBC, Hb and Hct levels exhibited significant increases after transportation (P<0.05). Mean daily glucose concentration showed an increase after transportation (P< 0.05). Mean total protein, albumine, AST and CK levels presented increases on day 2, after transportation (P< 0.05). Mean blood urea nitrogen, creatinine, total bilirubine, ALT, ALP, LDH and GGT did not show significant changes throughout the study. Mean Sodium (Na), Potassium (K) and Clor (Cl) levels showed alterations after transportation (P< 0.05), whereas Calsium (Ca) levels showed significant decreases after transportation (P< 0.05). 

Keywords

• Biochemistry,Hematology,Stallion,Stress,Transport

Kaynakça

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