İzole Edilmiş Rat Mesane Düz Kas Kontraksiyonları Üzerine Rosmarinus officinalis (Rosemary) Aköz Ekstraktının Etkileri

Öz

Rosmarinus officinalis (Rosemary) geleneksel tıpta yaygın olarak kullanılmaktadır ve o diüretik etkiye ve ileum, trakea, mide düz kasları üzerine gevşetici etkilere sahiptir. Fakat Rosemary’nin idrar kesesi düz kaslarının miyojenik aktiviteleri üzerine herhangi bir çalışmaya rastlanılmamıştır. Bu çalışmada izole edilmiş rat mesane düz kas kontraktilitesi üzerine Rosemary ekstraktının etkileri araştırılmıştır. Kas şeritleri Sprague Dawley cinsi erkek ratların mesanesinden hazırlandı. Hazırlanan kas şeritleri Krebs solüsyonu içeren izole organ banyosuna yerleştirildi. Kas şeritler ACh (10^-4 M) ve KCl (60 mM) ile uyarılarak kastırıldı ve bu değerler referans değer olarak kabul edildi. ACh ve KCl ile indüklenen kontraksiyonlar üzerine Rosemary ekstraktının farklı dozlarının (150, 300, 600 ve 1200 µg/mL ) etkileri belirlendi. Banyoda Rosemary’nin dozlarının varlığında ACh ve KCl ile indüklenen kontraksiyonların referans değerlerden farklılık arz etmediği belirlendi (P>0.05). Mevcut çalışmada, Rosemary ekstraktının çeşitli dozlarının rat idrar kesesi düz kaslarının myojenik aktivitesi üzerine etkisinin olmadığı görüldü. Çeşitli amaçlar için Rosemary kullanımının idrar kesesi düz kaslarının fizyolojik aktivitesi üzerine istenmeyen etkilerinin olmadığı düşünülebilir.

Anahtar Kelimeler

• Düz kas,İdrar kesesi,Rat,Rosmarinus officinalis,Sulu ekstrakt

The Effects of Rosmarinus officinalis (Rosemary) Aqueous Extract on Smooth Muscle Contractions in Isolated Rat Urinary Bladder

Öz

Rosmarinus officinalis (Rosemary) is widely used in traditional medicine and it has diuretic and spasmolytic effects on the ileum, trachea, and stomach smooth-muscle. However, a study the related effects of Rosemary on the myogenic activities of urinary bladder smooth-muscles has not been founded.  The present study investigated the effects of Rosemary extract on the smooth-muscle contractility of isolated rat urinary bladder. Muscle strips were prepared from urinary bladder of male Sprague Dawley rats. Prepared muscle strips were placed in an isolated organ bath containing Krebs solution. The muscle strips were pre-contracted by inducing with Ach (10^-4M) and KCl (60mM), and these values were considered to be reference values. The effects of the different doses (150, 300, 600 and 1200µg/mL) of Rosemary extract on ACh and KCl-induced contractions were determined. It was determined that no different from references values of the contractions induced by ACh and KCl in the presence of Rosemary doses in bath (P>0.05). In the present study, the various doses of Rosemary extract had no effect on myogenic activity in rat urinary bladder smooth-muscle. It may be conceive that the use of Rosemary for different purposes does not result in an undesirable effect on the physiological activities of bladder smooth-muscle.

Anahtar Kelimeler

• Aqueous extract,Urinary bladder,Rat,Rosmarinus officinalis,Smooth muscle

Kaynakça

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