Effects of L-Carnitine and Co-enzyme Q10 Together with ACE Inhibitors on Total Oxidant and Antioxidant Levels in L-Name Hypertensive Rats

Öz

In this study we aimed to find out the affects of giving ACE inhibitor with L-carnitine and Co-enzyme Q₁₀ on the oxidant-antioxidant system. For this purpose, 8 week-old and approximately 200-250 gr, 60 adult Sprague-Dawley rats were used. The animal were divided into three groups, served as 1 control and 2 experimental groups and each had 20 rats. Before starting the study, 75 mg/kg L-NAME given intraperitonelly to all groups for ten days to create hypertension. 10 mg/kg ACE inhibitor to the control group, 100 mg/kg L-carnitine+10 mg/kg ACE inhibitor to the L-carnitine group, 100 mg/kg Co-enzyme Q₁₀+10 mg/kg ACE inhibitor to the Co-enzyme Q₁₀ group was given. There were statistically significant decrease in the plasma levels of total oxidant in Co-enzyme Q10 and L-carnitine groups when compared to the control on 28^th day (P<0.05). There were no statistically significant difference between the groups in terms of plasma total antioxidant levels. Total oxidant levels of heart tissue in the L-carnitine group was statistically lower than the control group (P<0.001) on the day 14^th. There were no statistically significant difference on the total oxidant levels of heart tissue in Co-enzyme Q₁₀ group.The total oxidant levels of heart tissue of the experimental groups were statistically lower than the control group on 28^th day, and this decrease was more prominent in the L-carnitine group (P <0.001).  There were no statistically significant difference in total antioxidant levels of heart tissue. It was concluded that due to the antioxidant properties of L-carnitine and Co-enzyme Q10 in hypertension, these substances protects the heart and vascular system against free-radical damage, and also reducing the severity of secondary problems.

Anahtar Kelimeler

• ACE inhibitor,Antioxidant,Co-enzyme Q10,Hypertension,L-carnitine,Oxidant

L-Name Hipertansif Ratlarda Ace İnhibitörü ile Birlikte L-Karnitin ve Co-enzim Q10 Verilmesinin Total Oksidan ve Antioksidan Düzeyleri Üzerine Etkileri

Öz

Bu çalışma ile L-NAME hipertansif ratlarda ACE inhibitörü ile birlikte L- karnitin ve Co-enzim Q₁₀ verilmesinin total oksidan-antioksidan sistem üzerine etkilerinin araştırılması amaçlandı. Bu amaçla, ortalama 200-250 gr, 8 haftalık, 60 erişkin Spraque-Dawley rat kullanıldı. Ratlar her grupta 20 rat bulunacak şekilde, 1 kontrol ve 2 deney grubu olmak üzere 3 gruba ayrıldı. Çalışmaya başlamadan önce tüm gruplara 10 gün süreyle intraperitonel (i.p) olarak 75 mg/kg L- NAME verilerek hipertansiyon oluşturuldu. Hipertansiyon oluşturulduktan sonra, Kontrol grubuna 10 mg/kg ACE inhibitörü, L-karnitin grubuna 10 mg/kg ACE inhibitörü + 100 mg/kg L-karnitin, Co-enzim Q₁₀ grubuna 10 mg/kg ACE inhibitörü + 100 mg/kg Co-enzim Q₁₀ ip olarak verildi. Plazma total oksidan düzeylerinin hem L-karnitin hem de Co-enzim Q₁₀ gruplarında, kontrol grubuna göre 28. günde istatistiksel olarak önemli düzeyde düşük olduğu belirlendi (P<0.05). Plazma total antioksidan düzeylerinde gruplar arasında istatistiksel olarak bir farklılık gözlenmedi. Kalp dokusu total oksidan düzeylerinin 14. günde L-karnitin grubunun kontrol grubuna göre istatistiksel olarak daha düşük olduğu (P<0.001), Co-enzim Q₁₀ grubunda ise istatistiksel olarak önemli bir fark olmadığı belirlendi. 28. günde deneme gruplarının kalp dokusu total oksidan düzeylerinin kontrol grubuna göre istatistiksel olarak düşük olduğu, bu düşüşün L-karnitin uygulanan grupta daha belirgin olduğu görüldü (P<0.001). Kalp dokusu total antioksidan düzeylerinde istatistiksel olarak bir farklılık tespit edilmedi. Sonuç olarak, L-karnitin ve Co-enzim Q₁₀’nun hipertansiyonda kalbi ve damar sistemini serbest radikal hasarından koruyarak, oluşacak ikincil problemlerin şiddetini azaltabilecekleri kanaatine varıldı.

Anahtar Kelimeler

• ACE inhibitörü,Antioksidan,Co-enzim Q10,Hipertansiyon,L-karnitin,Oksidan

Kaynakça

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