Ratlarda Karbon Tetraklorür ile İndüklenen Karaciğer Toksikasyonu Üzerine Resveratrol’ün Koruyucu Etkisinin Histopatolojik ve TUNEL Metodu ile Araştırılması

Öz

Bu çalışmada, deneysel olarak karbon tetraklorür ile hepatotoksisite oluşturulan ratların karaciğer dokuları üzerine güçlü antioksidan etkisine sahip olan Resveratrol’ün, histopatoloji ve TUNEL metodu ile koruyucu etkinliğinin araştırılması amaçlanmıştır. Bu amaçla, 32 adet erkek Wistar cinsi Albino rat kullanıldı. Denekler rastgele seçilerek, 1. grup kontrol, 2. Grup CCl₄, 3. grup Resveratrol. 4. grup ise CCl₄+ Resveretrol olmak üzere 4 gruba ayrıldı. Deneme sonunda hayvanlar anestezi altında sakrifiye edilerek histopatolojik analizler için karaciğer örnekleri %10 formalin solüsyonuna alındı. Yapılan histopatolojik incelemede kontrol ve Resveratrol grubunda ratların karaciğerleri normal histolojik yapıda olduğu tespit edildi. CCI₄ grubunda ise karaciğerin periasiner bölgelerindeki hepatositlerde şiddeti hidropik dejenerasyon, steatozis ve kogulasyon nekrozu tesbit edildi. CCI₄+ Resveretrol grubunda ratların karaciğerlerinde periasiner bölgede hepatositlerde hafif hidropik dejenerasyona rastlanırken nekrotik hepatositlere hiç rastlanmadı. Apoptozisi belirlemek amacıyla TUNEL metodu kullanıldı. TUNEL sonuçlarına göre kontrol ve Resveratrol grubunda karaciğerlerde birkaç hücrede pozitiflik tespit edildi. CCI₄ grubunda karaciğerlerin periasiner bölgelerindeki hepatositlerde çok sayıda pozitif hücre belirlendi. CCl₄+Resveretrol grubunda ratların karaciğerlerinde periasiner bölgede çok az sayıda pozitif hücreye rastlandı. İstatiski olarak gruplar arasındaki fark önemli (P<0.05) bulundu. Sonuç olarak, histopatolojik ve immunohistokimyasal bulgulara göre CCI₄ neden olduğu oksidatif strese ve lipitperoksidasyonu sonucu oluşturulan karaciğer hasarına karşı Resveratrolün bu hasarı önemli düzeyde inhibe edici etkisinin olduğu belirlenmiştir.

Anahtar Kelimeler

• Apoptozis,Histopatoloji,Karbontetraklorür,Resveratrol,TUNEL metod

Investigation by Histopathological and TUNEL Method of the Protective Effect of Resveratrol on Carbon Tetrachloride Induced Liver Toxicity in Rats

Öz

In this study, it was aimed to investigate the protective effect of Resveratrol, which has a strong antioxidant effect on liver tissues, by histopathology and TUNEL method in experimental rats hepatotoxic with carbon tetrachloride. For this reason 32 male Wistar rats were used. Rats were randomly chosen and separated into 4 groups. First control, rats in second group CCl4, 3rd group Resveratrol 4th group CCl4+Resveretrol. At the end of the experiment, rats were euthanasied under anaesthesia and liver samples were taken in formalin solution 10%. According to histopathological examination, livers were in normal histopathological structure in the control and Resveratrol groups. In CCl₄ group, severe hydropic degeneration in hepatocytes localised in the center regions of the liver, steatosis and coagulation necrosis were detected. In CCl₄+Resveretrol group; while slight hydropic degeneration were detected in the central regions of livers, necrotic hepatocytes were not detected. TUNEL method were used in order to detect apoptosis. According to TUNEL results, positivity was detected in certain liver cells in control and Resveratrol group. Numerous positive cells were detected in central regions of livers in CCl₄ group. Few positive cells were detected in central region of rat livers in CCl₄+Resveretrol group. Differences in groups were statistically significant (P<0.05). As a result of histopathological and immunohistochemical examination, inhibitor effect of resvetratrol were determined against liver damage resulting from oxidative stress and lipiperoxidation caused by CCl₄.

Anahtar Kelimeler

• Apoptosis,Carbon tetrachloride,Histopathology,Resveratrol,TUNEL method

Kaynakça

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