Broylerlerde Enrofloksasinin Bazı Kan Değerleri Üzerine Etkilerinin Değerlendirilmesi

Öz

Bu çalışmada, enrofloksasinin yüksek dozlarının broyler piliçlerde bazı kan parametreleri üzerine etkileri araştırıldı. Toplam 50 adet, Ross-308, 1 günlük erkek broyler kullanıldı. Broylerler (10 günlük) dört gruba ayrıldı: Grup 1 (kontrol) ve Grup 2, 3 ve 4’e sırasıyla 10, 100 ve 200 mg/kg vücut ağırlığı olacak şekilde 30 gün boyunca enrofloksasin uygulaması yapıldı. Tedavi süresi sonunda kan değerlerini belirlemek amacıyla kan örnekleri toplandı. Kan parametreleri manuel metotla tespit edildi. RBC Grup 3 ve 4’te düşük; Hb, Grup 2’de yüksek, Grup 3 ve 4’te düşük; MCV düzeyleri Grup 3 ve 4’te yüksek; MCH, Grup 2, 3’te yüksek, Grup 4’te düşük; MCHC, Grup 3 ve 4’te düşük bulundu. WBC değerinde Grup 3 ve 4’te azalma vardı. Bu çalışmada, enrofloksasinin önerilen 10 mg/kg dozunun yaklaşık 10 ve 20 katının 30 gün boyunca ağız yoluyla uygulanmasının, broylerlerde bazı hematolojik değerleri değiştirdiği sonucuna varıldı. Bununla birlikte, broylerlerde anemi ve lökopeni şekillendiği belirlendi.

Anahtar Kelimeler

• Broyler, Enrofloksasin, Kan Parametreleri

Evaluation of Effects of Enrofloxacin on Some Haematological Parameters in Broilers

Abstract

In the present study, effects of enrofloxacin overdose on some haematological parameters of broiler chickens were investigated. A total of 50, Ross-308 one-day-old male broiler chickens were used. Broilers (10 days old) were divided into four groups: Animals in Group 1 (kept as control) and Group 2, 3 and 4 were administered 10, 100 and 200mg/kg body weight enrofloxacin for 30 consecutive days, respectively. Samples of blood were then collected to determine the haematological values. Haematological parameters were determined manually. Results revealed that the RBC decreased in Group 3 and 4; the Hb increased in Group 2 and decreased in Group 3 and 4; the MCV levels were higher in Group 3 and 4; the MCH increased in Group 2, 3 and decreased in Group 4; the MCHC decreased in Group 3 and 4. There were declines in the WBC in Group 3 and 4. The results suggest that the oral administration of enrofloxacin at approximately 10- and 20-fold of the proposed dosage of 10 mg /kg for up to 30 days altered some haematological parameters in broiler chickens. Additionally, it was shown that the resultant anaemia and leucopoenia occurred in broilers.

Keywords

• Broiler,Enrofloxacin,Haematological Parameters

Kaynakça

1. Al-Mayah AS., Al-Ahmed JA., 2005. Influence of antibiotic treatment on hematological aspect in chicken. Int. J. Poult. Sci., 4, 323-325.
2. Bertone AL., Tremaine H., Macoris DG., Simmons EJ., Ewert KM., Weisbrode SE., 1998. Effect of chronic systemic enrofloxacin solution on physical musculoskeletal and histological parameters in adult horse. AAEP Proceedings, 44, 252-253. of an injectable
3. Burkhardt JE., Hill MA., Carlton WW., Kesterson JW, 1990. Histologic and histochemical changes in articular cartilages of immature beagle dogs dosed with difloxacin, a fluoroquinolone. Vet. Pathol., 27, 162- 170.
4. Carreras I., Castellari M., Garcia Regueiro JA., Guerrero L., Esteve-Garcia E., Sarraga C., 2004. Influence of enrofloxacin administration and acetate supplemented diets on oxidative stability of broiler tissues. Poult. Sci., 83, 796-802. -tocopheryl
5. Christ W., Lehnet T., Ulbrich B., 1988. Specific toxicologic aspect of the quinolones. Rev. Infect. Dis., 10, 141- 146.
6. Doyle ME., 2001. Alternative to antibiotics use for growth promotion in animal husbandry. Food Research Institute, Madison.
7. Ellakany HF., Abu El-Azm IM, Bekhit AA., Shehawy MM., 2007. Studies on the effects of enrofloxacin overdose on different health parameters in boiler chickens. BS. Vet. Med. J., 5th Scientific Conference 176-186.
8. Giguere S., Sweeney RW., Habecker PL., Richardson DW., 1999. enrofloxacin in adult horses. J. Vet. Pharmacol. Ther., 22, 343-347. orally administered

9. Gilbersto S., Jones DR., 1972. Effect of nalidixic acid on blood. Brit. J. Urol., 44, 503
10. Gough AW, Kasali OB., Sigler RE., Baragi V., 1992. Quinolone arthropathy-Acute toxicity to immature cartilage. Toxicol. Pathol., 20, 436-449.
11. Hooper DC., Wolfson JS., 1993. Adverse effects. In “Quinolones Antimicrobial Agents”. Eds., DC Hooper and J Wolfson, American Society for Microbiology, Washington, USA.
12. Kilic FS., Batu O., Yildrim E., Erol K., Deliorman S., 2003. Ciprofloxacin inflammatory response in rats. J. Health. Sci., 49, 391-394. supress the
13. Konuk T., 1981. Pratik Fizoloji, 2. baskı. Ankara Üniversitesi Veteriner Fakültesi Yayınları, Ankara.
14. Lubran MM., 1989. Haematological side effects of drug. Ann. Clin. Lab. Sci., 19, 114-121. Maguire RB., Stroce EG., Yearlsey M., 1994. Haematological anemia and acute renal failure associated with temafloxacin-dependent antibodies. Am. J. Hematol., 46, 363-366.
15. Martinez M., McDermott P., Walker R., 2006. Pharmacology of fluoroquinolones: A perspective for the use in domestic animals. Vet. J., 172, 10-28.
16. Mohandas N., Kim YR., Tycko DH., Orlik J., Wyatt J., Groner W., 1986. Accurate and independent measurement concentration of individual red cells by laser light scattering. Blood, 68, 506-513. and hemoglobin
17. Muhammad NO., Oloyede OB., 2009. Haematological parameters of broiler chicks fed Aspergillus niger- fermented Terminalia catappa seed meal-based diet. Global J. Biotechnol. Biochem., 4, 178-183.
18. Sin CP., Wang R., Chu V., 2002. Liquid chromatographic determination of fluoro-quinolones in egg albumin and egg yolk of laying hens using fluorometric detection. J. Agric. Food. Chem., 50, 4452-4455.
19. Talebi A., Asri-Rezaei S., Rozeh-Chai R., Sahraei R., 2005. Comparative studies on haematological values of broiler strains (Ross, Cobb, Arbor-acres and Arian). Int. J. Poult. Sci., 4, 573-579.
20. Toghyani M., Tohidi M., Gheisari AA., Tabeidian SA., 2010. Performance, immunity, serum biochemical and hematological parameters in broiler chicks fed dietary thyme as alternative for an antibiotic growth promoter. Afr. J. Biotechnol., 9, 6819-6825.
21. Traş B., Maden M., Baş L., Elmas M., Yazar E., Civelek T., 2001. Investigation haematological side-effects on enrofloxacin in dogs. J. Vet. Med. A, 48, 59-63. biochemical and