Yetişkin Bıldırcınların Gastrointestinal Sisteminde Gastrin, Somatostatin ve Serotonin Salgılayan Hücreler Üzerine İmmunohistokimyasal Bir Araştırma

Öz

Bu araştırmada, yetişkin bıldırcınların gastrointestinal sistemindeki gastrin (G hücreleri), somatostatin (D hücreleri) ve serotonin (EC hücreleri) immunopozitif hücrelerin dağılımı, yerleşimi ve sıklığının immunohistokimyasal yöntemlerle belirlenmesi amaçlandı. Çalışmada, 10 adet sağlıklı yetişkin bıldırcının glanduler ve musküler mideleri, duodenum, jejunum ve ileumları materyal olarak kullanıldı. Alınan doku kesitleri streptavidin biotin peroksidaz yöntemi ile immunohistokimyasal olarak boyandı. Gastrin ve serotonin immunopozitif hücrelerin glanduler ve musküler midelere göre ince barsaklarda daha fazla olduğu belirlendi. Bununla birlikte, somatostatin immunopozitif hücreler mide ve ince barsakların tamamında çok az sayıda görüldü. Ayrıca, G, D ve EC hücrelerinin her iki midede genellikle kapalı tip, ince barsak bölümlerinde ise hem açık hem de kapalı tip endokrin hücreler halinde bulunduğu da tespit edildi. Sonuç olarak, bıldırcınların glanduler ve musküler mideleri, duodenum, jejunum ve ileumlarında G, D ve EC hücrelerinin değişen sayılarda bulunduğu belirlendi. Ayrıca, duodenum ve ileum mukozasında, G ve EC hücrelerinin D hücrelerine göre daha fazla bulunduğu da tespit edildi.

Anahtar Kelimeler

• Bıldırcın, Enteroendokrin hücreler, Gastrointestinal sistem, İmmunohistokimya

Kaynakça

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