Prunus laurocerasus L. Meyve Ekstraktının Sıçanlarda İndometazin ile İndüklenen Gastrik Ülsere Karşı Koruyucu Etkisi

Öz

Bu çalışmada Prunus laurocerasus meyve ekstraktının, non-steroid antiinflamatuar ilaçlardan biri olan indometazin kaynaklı gastrik ülser modelinde koruyucu etkilerinin araştırılması amaçlanmıştır. Deney hayvanları dört gruba ayrıldı (Kontrol, Prunus laurocerasus, İndometazin ve Omeprazol grupları). I. ve III. gruba izotonik tuzlu su, II. gruba 500 mg/kg Prunus laurocerasus ekstraktı ve IV. gruba 5 mg/kg omeprazol oral olarak verildi. Çalışmanın 11. gününde II, III ve IV. gruplara tek doz 100 mg/kg indometazin oral olarak uygulandı. Siklooksijenaz - 2 (COX-2) ve C - reaktif protein (CRP) seviyeleri, indometazin grubunda önemli şekilde artarken (P<0.001), Prunus laurocerasus (sırasıyla P<0.05, P<0.001) ve omeprazol uygulamaları ile önemli şekilde azaldı (sırasıyla P<0.01, P<0.001). İndometazin grubunda interlökin - 6 (IL-6) ve tümör nekrozis faktör - alfa (TNF-α) düzeylerinde artış olduğu belirlendi (P<0.05). Hem Prunus laurocerasus hem de omeprazol uygulaması ile IL-6 ve TNF-α düzeylerinin azaldığı, ancak bu azalışların istatistiksel olarak anlamlı olmadığı tespit edildi. Sonuç olarak, Prunus laurocerasus’un, mide ülserinde CRP ve COX-2 seviyelerini düşürmede piyasadaki standart ilaçlardan biri olan omeprazol benzeri etki gösterdiği tespit edilmiştir.

Anahtar Kelimeler

• C-reaktif protein,Gastrik ülser,Prunus laurocerasus,Proinflamatuar sitokinler,Siklooksigenaz-2

Protective Effect of Prunus laurocerasus L. Fruit Extract Against Indomethacin-Induced Gastric Ulcer in Rats

Abstract

In the present study, it was aimed to investigate the protective effects of Prunus laurocerasus fruit extract in the indomethacine-induced gastric ulcer model, which one of the non-steroid anti-inflammatory drugs. Experimental animals were divided into four groups (Control, Prunus laurocerasus, Indomethacine and Omeprazole groups). The groups I and III was given isotonic saline, the group II was given 500 mg/kg Prunus laurocerasus extract and the group IV was given 5 mg/kg omeprazole orally. On the 11th day of the study, a single dose of 100 mg/kg indomethacine was administered orally to the II, III and IV groups. The levels of cyclooxygenase - 2 (COX-2) and C - reactive protein (CRP) increased significantly in indomethacine group (P<0.001), while significantly decreased with the application of Prunus laurocerasus (P<0.05, P<0.001 respectively) and omeprazole (P<0.01, P<0.001 respectively). It was determined that interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) levels were increased in the indomethacin group (P<0.05). It was detected that IL-6 and TNF-α levels decreased with both Prunus laurocerasus and omeprazole administration, but these decreases were not statistically significant. In conclusion, it has been detected that Prunus laurocerasus has an omeprazole-like effect, which is one of the standard drugs in the market, when lowering CRP and COX-2 levels in gastric ulcer.

Keywords

• Gastric ulcer,Prunus laurocerasus,Proinflammatory cytokines,C - reactive protein,Cyclooxygenase - 2,Prunus laurocerasus

Kaynakça

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