The relationships of Glucose-6-Phosphate Dehydrogenase, Glutathione Peroxidase, Nicotinamide Adenine Dinucleotide Phosphate and Glutathione with Milk Quality Parameters

Yıl 2019, Cilt: 14 Sayı: 2, 193 - 200, 25.10.2019

Zeynep Akın Pınar Peker Akalın Filiz Kazak , Yaşar Ergün Nuri Başpınar

https://izlik.org/JA55XT33CN

Öz

In this study, it was aimed to determine the relationship between with G6PD, GPx, NADPH, rGSH and milk yield and milk quality, to evaluate the effect of these parameters on milk quality, in Holstein cattle. Milk samples were collected from 20 healthy Holstein cattle. Milk yield was obtained from routine farm datum. The analyses of milk fat %, protein %, lactose %, non-fat dry matter %, urea % and casein % were held by “milk components measuring instrument”, and G6PD and GPx activities in milk cell supernatants along with NADPH, rGSH and the total protein levels were assessed by spectrophotometric methods. Milk lactose levels (%) were negatively correlated with milk cell G6PD activity (r=-0.574, P<0.05) and NADPH levels (r=-0.485, P<0.05). Milk cell G6PD activity (r=0.708, P<0.01) and milk cell NADPH levels (r=0.609, P<0.01) were positively correlated with somatic cell count. A positive correlation (r=0.630, P<0.01) was determined between the somatic cell count and milk cell NADPH levels. In conclusion, there was no correlation between milk yield and milk quality parameters with the related parameters, except for lactose levels, in healthy cattle milk. It is suggested to investigate the relationship between lactose and G6PD with further studies.

Anahtar Kelimeler

G6PD , NADPH , Milk quality parameters , Somatic cell count

Glukoz-6-Fosfat Dehidrogenaz, Glutatyon Peroksidaz, Nikotinamid Adenin Dinükleotid Fosfat ve Glutatyon ile Süt Kalite Parametreleri Arasındaki İlişkiler

https://izlik.org/JA55XT33CN

Öz

Bu çalışmada; Holstein ırkı sığırlarda süt G6PD, GPx, redükte NADPH ve rGSH düzeyleri ile süt verimi ve süt kalitesi ile arasındaki ilişkilerin belirlenmesi, böylelikle ilgili parametrelerin süt kalitesi üzerindeki etkilerinin araştırılması amaçlanmıştır. Klinik olarak sağlıklı 20 adet Holstein ırkı sığırdan süt örnekleri toplanmıştır. Süt verimi çiftlikte tutulan rutin kayıtlardan tespit edilmiştir. Sütlerde kalite parametreleri olan süt yağı %, protein %, laktoz %, kuru madde %, yağsız kuru madde %, üre % ve kazein % analizleri süt komponentleri ölçüm cihazı ile, süt hücre süpernatantlarında G6PD ve GPx aktiviteleri ile NADPH, rGSH ve total protein düzeyleri ise spektrofotometrik yöntemlerle belirlenmiştir. Süt laktoz düzeyleri ile süt hücre G6PD aktivitesi (r=-0.574, p<0.05) ve NADPH düzeyleri (r=-0.485, P<0.05) arasında negatif korelasyonlar belirlenmiştir. Süt somatik hücre sayısı ile süt hücre G6PD aktivitesi (r=0.708, P<0.01) ve süt hücre NADPH düzeyleri (r=0.609, P<0.01) arasında pozitif korelasyonlar belirlenmiştir. Süt somatik hücre sayısı ile süt hücre NADPH düzeyleri arasında pozitif bir korelasyon (r=0.630, P<0.01) belirlenmiştir. Sonuç olarak sağlıklı inek sütünde, anılan parametreler ile süt verimi ve süt kalite parametreleri arasında, laktoz ve G6PD dışında, belirgin bir korelasyon belirlenmemiştir. Laktoz ile G6PD arasındaki bu ilişkinin yapılacak çalışmalar ile detaylandırılması önerilmektedir.

Anahtar Kelimeler

G6PD , NADPH , Somatik hücre sayısı , Süt kalite parametreleri

Kaynakça

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