The Effect of Different Concentrations of Bovine Serum Albumin on Sperm Quality and DNA Damage in Short Term Stored Ram Semen

Abstract

In the present research the aim was emphasized to find out the effects of bovine serum albumin (BSA) on quality of sperm and DNA damage at 0, 24 th, 48 th and 72 th hours in Merino ram semen at short term preservation (5°C). Ejaculates were obtained from five Merino Rams which were collected by artificial vagina and all the samples were pooled at 37°C for detection. All the procedures were applied six times concurrently. Different concentrations (0, 2, 4 and 6 mg/ml) of BSA with Tris-based extender was used for dilution on semen samples. Sperm motility, abnormality, plasma membrane integrity, acrosome integrity and % DNA in tail (COMET assay) content were measured and analyzed. The extender with 4 mg/ml of BSA had both higher motility percentages and plasma membrane integrity, when compare with the control, up to 72 hours (P<0.05). At the 24th and 48th hours of the study, DNA damage was lower in all doses of BSA than in the control group. In addition, in terms of abnormal spermatozoon rates, all doses of BSA were lower than the control group at 24, 48 and 72 hours (P<0.05). In conclusion, it was found to be especially useful to add 4 mg / ml BSA for the ram semen parameters in short term storage.

Keywords

• BSA,DNA integrity,liquid storage,ram semen

Kısa Süreli Saklanan Koç Spermasına İlave Edilen Sığır Serum Albumininin (BSA) Farklı Dozlarının Sperma Kalitesi ve DNA Hasarı Üzerine Etkisi

Öz

Bu çalışmanın amacı kısa süreli (5 °C)’de saklanan Merinos koç spermasında 0, 24, 48 ve 72. saatlerde sığır serum albümininin (BSA) sperma kalitesi ve DNA hasarı üzerindeki etkilerini belirlemektir. Ejekülatlar beş baş Merinos ırkı koçtan suni vajen ile toplandı ve değerlendirmeler 37 °C'de yapıldı. Tüm çalışma altı kez tekrar edildi. Toplanan koç sperması örnekleri dörde bölündü ve Tris temelli sulandırıcı ile sulandırılan spermaya (0, 2, 4 ve 6 mg/ml) dozlarında BSA eklendi ve motilite, anormal spermatozoon oranı, plazma membran bütünlüğü ve canlılık, akrozom bütünlüğü ve DNA hasarı (COMET testi) belirlendi. Çalışma gruplarından 4 mg/ml BSA içeren grup, 72 saatlik depolamaya kadar, kontrol ile karşılaştırıldığında daha yüksek motilite yüzdeleri, plazma membran bütünlüğü ve canlılık gösterdi (P<0.05). Araştırmamızın 24 ve 48. saatlerinde DNA hasarı BSA’nın tüm dozlarında kontrol grubuna göre daha düşük bulundu. Bununla beraber anormal spermatozoon oranları yönünden BSA’nın tüm dozlarında 24, 48 ve 72 saatler sonunda kontrol grubuna göre daha düşük değerler olduğu görüldü (P<0.05). Sonuç olarak, kısa süreli saklamada koç sperma parametreleri için özellikle 4 mg/ml BSA eklemenin yararlı olduğu görülmüştür.

Anahtar Kelimeler

• BSA,DNA hasarı,kısa süreli saklama,koç sperması

Kaynakça

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