Direkt ELISA ile Tanısı Konmuş, Enterotoksijenik E. coli (ETEC) K99+ ile Enfekte Manda Yavrularındaki (Malak) Bazı Klinikopatolojik Değişiklikler

Öz

Bu çalışmanın amacı, Eylül 2018 - Nisan 2019 tarihleri arasında, direkt ELISA ile Enterotoksijenik E. coli (ETEC) K99+ tespit edilen doğal buffalo buzağılarındaki (1-15 gün arası) klinikopatolojik (hematolojik ve biyokimyasal) bazı değişiklikleri belirlemekti. Toplam 40 dışkı örneği (enfekte hayvandan 30 örnek ve klinik olarak sağlıklı Buffalo buzağılarından 10 örnek) direkt ELISA ile incelendi. Diyareik hayvanlardan alınan altı numune (%20), E. coli (ETEC) K99+ için pozitifti. Kontrol grubundaki hayvanlarla karşılatırıldığında, Enterotoksijenik E. coli (ETEC) K99+ ile enfekte olmuş hayvanların kan örneklerindeki kan gazı analizi kan pH'ı, kısmi oksijen konsantrasyonu basıncı (PO2), kısmi karbondioksit konsantrasyonu (PCO2), baz fazlalığı (BE) ve ayrıca elektrolitler: sodyum (Na+), klorür (CL-), bikarbonat (HCO3-), Kalsiyum (Ca++) ve iyonlaştırılmamış Kalsiyum (nCa++) iyonları konsantrasyonlarında önemli azalmaya, hemoglobin konsantrasyonu (tHB), hematokrit (PCV) ve anyon açığı (AG) değerlerinde ise önemli bir artışa neden oldu. Enterotoksijenik E. coli (ETEC) K99+ ile enfekte olmuş doğal buffalo buzağılarındaki ana klinikopatolojik değişikliklerden; elektrolitler, kangazları, Kalsiyum (Ca++) ve iyonize olmayan Kalsiyum (nCa++) iyonları konsantrasyonları önemli ölçüd eazaldı.The main clinicopathological changes in native buffalo calves infected  with enterotoxigenic E. coli (ETEC) K99+ were significant  decrease in electrolytes, blood gases,Calcium (Ca⁺⁺) and nonionized Calcium (nCa⁺⁺) ions concentrations.

Anahtar Kelimeler

• Direkt ELISA,E. coli,Kan Gazları,, Klinik Patoloji,Manda Yavrusu (Malak)

Some Clinicopathological Some Clinicopathological Changes in Buffalo Calves Infected with Enterotoxigenic E. coli (ETEC) K99+ Detected by Direct ELISA in Buffalo Calves Infected with Enterotoxigenic E. coli (ETEC) K99+ Detected by Direct ELISA

Öz

The objective of recurrent study was to determine the some clinicopathological (hematological and biochemical) changes in native buffalo calves (aged between 1-15 day) infected with enterotoxigenic E. coli (ETEC) K99+ detected by direct ELISA, during the period between September 2018- April 2019. A total of 40 fecal samples (30 samples from infected animal and 10 samples from clinically healthy Buffalo calves) was examined by direct ELISA. Six samples form diarrheic animals (20%) were positive for E. coli (K99). The blood gas analysis of blood samples revealed significant decrease in the means of the blood pH, Partial pressure of oxygen concentration (PO2 ), Partial pressure of carbon dioxide concentration (PCO2), base excess (BE), as well as electrolytes: sodium (Na+), chloride (CL-), bicarbonate (HCO3-), calcium (Ca++) and nonionized calcium (nCa++) ions concentrations, while a significant increase in the mean of hemoglobin concentration (tHB), packed cell volume (PCV) and anion gap (AG) in the buffalo calfs infected with enterotoxigenic E. coli (ETEC) K99+comparison with the control animals. The main clinicopathological changes in native buffalo calves infected with enterotoxigenic E. coli (ETEC) K99+ were significantly decrease in electrolytes, blood gases, Calcium (Ca++) and nonionized Calcium (nCa++) ions concentrations

Anahtar Kelimeler

• Blood Gases,Buffalo Calves,Clinical Pathology,Direct ELISA,E. coli

Kaynakça

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