Wistar Albino Sıçanlarda Michauxia campanuloides L’Hér.’in Bazı Sperma Parametreleri Üzerine Etkisi

Öz

Bu çalışmanın amacı sıçanlarda Michauxia campanuloides L’Hér. kullanımının bazı sperma parametreleri üzerine etkisini araştırmaktır. Sunulan çalışmanın bitki materyali antioksidan özellikli Michauxia campanuloides L’Hér.'dir. Çalışmanın hayvan materyalini 18 adet erkek Wistar albino sıçan oluşturmuştur. Sıçanlar kontrol (K; n=6), uygulama 1 (U1; n=6) ve uygulama 2 (U2; n=6) olmak üzere üç gruba ayrılmış ve M. campanuloides L’Hér.'in liyofilize sulu ekstresi U1 grubundaki sıçanlara 20 mg/kg ve U2 grubundaki sıçanlara 40 mg/kg dozunda 21 gün süreyle oral yolla günlük uygulandı. Çalışma sonunda tüm sıçanlar kan serumu total antioksidan kapasitesi (TAK), sperma yoğunluğu, sperma motilitesi, anormal spermatozoon oranı ile testis, epididimis, seminal bez ve ventral prostat ağırlığı yönünden muayene edildi. Sperma yoğunluğu K ve U1 gruplarına göre U2 grubunda anlamlı düzeyde daha yüksek bulundu (P=0,002). Anormal spermatozoon oranının K grubuna göre U1 ve U2 grubunda anlamlı düzeyde daha düşük olduğu tespit edildi (P=0,003). Seminal bez ağırlığı K grubuna göre U1 ve U2 gruplarında istatistiksel olarak daha yüksek ölçüldü (P=0,016). Sonuç olarak, M. campanuloides ekstresinin oral tüketimi sıçanlarda sperma kalitesinde bir artış sağlamaktadır.

Anahtar Kelimeler

• Antioksidan
• Michauxia campanuloides
• Sıçan
• Sperma parametreleri

The effect of Michauxia campanuloides L’Hér. on Some Semen Parameters in Wistar Albino Rats

Öz

The aim of this study was to investigate the effect of Michauxia campanuloides L’Hér. on some semen parameters in rats. The plant material of the study is Michauxia campanuloides L'Hér. having antioxidant property. The animal material of the study consisted of 18 male Wistar albino rats. The rats were divided into three groups as control (C; n=6), treatment 1 (T1; n=6) and treatment 2 (T2; n=6). Lyophilized aqueous extract of M. campanuloides L’Hér. was administered orally at a dose of 20 mg/kg/day for rats in the T1 group and 40 mg/kg/day for rats in the T2 group for 21 days. At the end of the study, all rats were examined for blood total antioxidant capacity (TAC), semen density, semen motility, abnormal spermatozoon ratio along with testicular, epididymal, seminal gland and ventral prostate weight. Semen density was significantly higher in the T2 group compared to the C and T1 groups (P=0.002). Rates of abnormal spermatozoa were significantly lower in T1 and T2 groups than the C group (P=0.003). The weight of seminal gland was significantly higher in the T1 and T2 groups compared to the C group (P=0.016). In conclusion, oral consumption of M. campanuloides extract provides an increase in semen quality in rats.

Anahtar Kelimeler

• Antioxidant
• Michauxia campanuloides
• Rat
• Semen parameters

Kaynakça

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