Çekal Ligasyon ve Delmeye Bağlı Polimikrobiyal Sepsis Sıçan Modelinde 6-Shogaol'ün Böbrek Dokusu Üzerine Renoprotektif Etkileri

Öz

Sepsis, yüksek mortalite ile çoklu organ disfonksiyonuna ilerleyebilen hayatı tehdit eden bir sendromdur. Bağırsak bariyerinde gerçekleşen bir deformasyon veya delinme sepsise bağlı çoklu organ yetmezliğinde önemli bir rol oynar. 6-Shogaol'ün (6-SHO) çekal ligasyon ve delmeye (CLD) bağlı böbrek hasarındaki koruyucu etkileri araştırıldı. Deney hayvanları 4 gruba ayrıldı ve şu şekilde planlandı; sham, CLD, CLD + DMSO ve CLD + 6-SHO. CLD + 6-SHO grubunda, CLD'den önce 6-SHO enjekte edildi (20 mg/kg, intraperitoneal). Toplanan böbrek dokuları homojenize edilerek antioksidan moleküller (total antioksidan kapasite (TAS) ve süperoksit dismütaz (SOD)), oksidan moleküller (total oksidan kapasite (TOS), malondialdehit (MDA) ve myeloperoksidaz (MPO)) ve pro-inflamatuar sitokinlerin (tümör nekroz faktör-α (TNF-α) ve interlökin-1β (IL-1β)) düzeyleri ölçüldü. İstatistiksel analizler ANOVA ile yapıldı. CLD gruplarında antioksidan moleküller; seviyeleri azalırken oksidan molekülerin seviyeleri yükseldi. Benzer şekilde pro-inflamatuar sitokin seviyeleri de CLD gruplarında yüksek bulundu. 6-SHO ile tedavi edilen grupta oksidan moleküllerin ve proinflamatuar sitokin seviyelerinin azaldığı ve antioksidan moleküllerin seviyeleri yüksek bulundu. 6-SHO uygulamasının CLD ile indüklenen polimikrobiyal sepsis modeli tarafından üretilen oksidatif böbrek hasarına karşı etkili olduğunu gösterilmiştir.

Anahtar Kelimeler

• 6-Shogaol
• Böbrek
• Çekal ligasyon ve delinme
• İnflamasyon
• Oksidatif stres

Reno-Protective Effects of 6-Shogaol on Kidney Tissue in Cecal Ligation and Puncture-Induced Polymicrobial Sepsis Rat Model

Öz

Sepsis is a life-threatening syndrome that may lead to multiple organ dysfunction with high mortality. A deformation or puncture in the intestinal barrier plays an important role in sepsis-induced multiple organ failure. The protective effects of 6-Shogaol (6-SHO) on renal injury caused by cecal ligation and puncture (CLP) was investigated. Experimental animals were divided into 4 groups and planned as follows; sham, CLP, CLP+DMSO, and CLP+6-SHO. In CLP + 6-SHO group, 20 mg/kg 6-SHO was administered intraperitoneally before the CLP model. The levels of antioxidant molecules (total antioxidant status (TAS) and superoxide dismutase (SOD)), oxidant molecules (total oxidant status (TOS), malondialdehyde (MDA) and myeloperoxidase (MPO)) and proinflammatory cytokines (tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β)) were measured by homogenizing in collected kidney tissues. Statistical analyzes were performed via ANOVA. In CLP groups, antioxidant molecule levels decreased while oxidant molecule values elevated. Similarly, proinflammatory cytokine levels were found high in CLP groups. In CLP+6-SHO group, oxidant molecules, MPO, and proinflammatory cytokine levels diminished, and antioxidant molecules increased. 6-SHO application has been shown to be effective against oxidative renal injury caused by the CLP-induced polymicrobial sepsis model.

Anahtar Kelimeler

• 6-Shogaol
• Cecal ligation and puncture
• Kidney
• Inflammation
• Oxidative stress

Kaynakça

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