Kınalı Keklik (Alectoris Chukar) Dalağının Morfolojik ve Histolojik Yapısının İncelenmesi

Öz

Dalak; ventriculus ve proventriculus’un birleştiği yerin sağında yer alan, bazı kan hücrelerinin depolanması, üretilmesi ve yıkımı gibi birçok görevi bulunan lenfoid bir organdır. Çalışmada kınalı keklik (Alectoris chukar)'lerde dalağın morfolojik ve histolojik özelliklerinin araştırılması amaçlanmıştır. Yapılan bu çalışmada 8 adet Kınalı keklik (Alectoris chukar) kullanıldı. Kekliklerden diseke edilen dalak dokularının morfolojik yönden incelenmek üzere ağırlığı, eni, uzunluğu, yüksekliği ölçülerek %10'luk formaldehit çözeltisinde 72 saat süreyle tespit edildi. Tespit olan bu dokular histolojik incelemeler için dehidratasyon ve şeffaflaştırma işlemlerine tabii tutuldu. Daha sonra parafin bloklara gömülerek 5μm kalınlığında kesitler alındı. Alınan kesitler Crossman’ın modifiye üçlü boyası ile boyandı ve histolojik yapısı incelendi. Morfolojik ölçümlerde ise dalağın boyunun 10.32±0.78 mm, eninin 6.60±0.50 mm, yüksekliğinin 5.55±0.26 mm, ağırlığının 0.26±0.06 gr değerleri arasında olduğu belirlendi. Kınalı keklik dalağının kalın bir kapsül ile sarılmış olduğu gözlenip, içerisinde küçük trabeküllerin bulunduğu belirlendi. Kırmızı pulpa ve beyaz pulpanın paranşim içerisinde dağınık halde olduğu, beyaz pulpa'nın retiküler hücreler, retiküler lifler ve lenfositlerden oluştuğu gözlendi. Histolojik incelemede, kırmızı pulpa'nın çok sayıda kan damarı, lenfosit, makrofaj, venöz sinüsler ile retiküler hücrelerin anastomozlarından oluştuğu belirlendi. Elde edilen veriler doğrultusunda kınalı keklik dalağının morfolojik yönden diğer kuş türleriyle farklılıklar gösterdiği bununla beraber histolojik olarak ise tavuk ve bıldırcın dalağıyla benzer olduğu gözlemlendi.

Anahtar Kelimeler

• Kınalı keklik
• Dalak
• Morfoloji

Investigation of Morphological and Histological Structure of Red-Legged Partridge (Alectoris Chukar) Spleen

Öz

The spleen of birds is located in the right side of the junction between the ventriculus and proventriculus, which is a lymphoid organ and responsible for many functions such as storing, production, and destruction of some blood cells. The aim of this study was to investigate the morphologic and histologic structures of the spleen in red-legged partridge (Alectoris chukar). In the present study, eight red-legged partridges were used. The spleens were removed from red-legged partridges and were measured in point of width, length, and weight for morphologic examination. The spleens were fixed into 10% formaldehyde solution for 72 hours. The fixed tissues were dehydrated and cleaned for histologic examinations. Then, these tissues were embedded in paraffin blocks and sectioned in a thickness of 5μm. Obtained sections were stained with Crossman Modified Triple staining and examined for their histologic structure. In the morphologic analysis, the average length of spleens was found as 10.32 ± 0.78 mm, the average width of spleens was found as 6.60 ± 0.50 mm, the average height of spleens was found as 5.55 ± 0.26 mm, and the average weight of spleens was found as 0.26 ± 0.06 g; in histologic analysis, spleen of red-legged partridge was surrounded with a thick capsule, and a few trabeculae were determined. Red and white pulps were scattered in the parenchyma; white pulps consisted of reticular cells, fibers, and lymphocytes. Red pulps consisted of macrophages, lymphocytes, venues sinuses, and reticular cells. In conclusion, morphologic features of the spleen in red-legged partridge were different from other bird species, but histologic structures were similar to chicken and quail spleens.

Anahtar Kelimeler

• Red-legged partridge
• spleen
• morphology

Kaynakça

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