c-erb B2 ve UGCG Köpeklerin Meme Karsinomlarında Güvenilir İmmunohistokimyasal Markerlar mıdır?

Öz

Bu çalışmada, köpek meme tümörlerinde c-erb B2, UGCG ekspresyonları ve tümör boyutu arasında ilişki kurmak amaçlandı. Çalışmanın materyali; 2012-2020 yılları arasında Anabilim Dalımıza getirilen 10 dişi köpekten alınan meme kanseri dokusu örneklerinden oluştu. İmmünohistokimyasal yöntem olarak Avidin-Biotin Peroksidaz yöntemi kullanıldı. Tüm köpek meme tümörü vakaları c-erb B2 ve UGCG reaksiyonları yönünden immun pozitifti. c-erb B2 ve UGCG immun pozitif hücre sayısındaki artışın tümör boyutuna paralel olduğu tespit edildi. Neoplastik epitel hücrelerde membranöz ve sitoplazmik c-erb B2 immün pozitif boyamalar tespit edildi. Reaksiyonların şiddeti orta ila şiddetli düzeydeydi. Neoplastik epitel hücrelerinin sitoplazmasında şiddetli koyu kahverenginde sitoplazmik seramid glukoziltransferaz boyaması tespit edildi. C-erb B2 ve UGCG immün pozitif hücre sayısındaki artışın tümör boyutuna paralel olduğu ortaya konuldu. Sonuç olarak, köpek meme tümörlerinde önemli bir tanı parametresi olan tümör boyutu ile doğru orantılı olarak c-erb B2 ve UGCG pozitif hücre sayısındaki bu artış, bize köpek meme tümörlerinin teşhisinde c-erb B2 ve UGCG'nin güvenilir immunohistokimyasal belirteçler olabileceğini düşündürdü. Ek olarak bu belirteçlerin, farklı kanser tanı parametreleri doğrultusunda kapsamlı bir şekilde değerlendirilmesi elzemdir.

Anahtar Kelimeler

• c-erb B2
• köpek
• meme tümörü
• ugcg

Are c-erb B2 and UGCG Reliable Immunohistochemical Markers Of Canine Mammary Carcinomas?

Öz

In this study, we aimed to correlate c-erb B2, UGCG expressions and tumor size in canine mammary tumors. The material of this study consisted of tissue samples of mammary carcinomas taken from ten female dogs brought to our department between 2012-2020. Avidin-Biotin Peroxidase method was used as an immunohistochemical method. All canine mammary tumor cases were immune positive for c-erb B2 and UGCG reactions. We detected membranous and cytoplasmic c-erb B2 immune positive staining in the neoplastic epithelial cells. Reactions were moderate to strong in severity. We observed strong dark brown cytoplasmic ceramide glucosyltransferase staining in the cytoplasm of neoplastic epithelial cells. We found that the increase in the number of c-erb B2 and UGCG immune positive cells paralleled the tumor size. In conclusion, the increase in the number of c-erb B2 and UGCG positive cells in direct proportion to the tumor size, which is an important diagnostic parameter in canine mammary tumors, made us think that c-erb B2 and UGCG could be reliable immunohistochemical markers in the diagnosis of canine mammary tumors. In addition, these markers must be evaluated comprehensively in line with different cancer diagnosis parameters.

Anahtar Kelimeler

• canine
• c-erb B2
• mammary tumor
• ugcg

Kaynakça

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